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Cited by 0Bacteriophages and microbial interactionsLegionella and Acanthamoeba researchPlant and Fungal Interactions Research

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Genomic and structural insights into Jyvaskylavirus, the first giant virus isolated from Finland

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Almeida, Gabriel Magno de Freitas, Arriaga, Iker, de Azevedo, Bruna Luiza, Leppänen, Miika, Abrahão, Jonatas S, Andreani, Julien, Zabeo, Davide, Ravantti, Janne J, Abrescia, Nicola GA, Sundberg, Lotta-Riina·2025·eLife
DOI 10.7554/elife.103492.3

Preprint — published as 10.7554/elife.103492 · Paper page

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Abstract

Giant viruses of protists are a diverse and likely ubiquitous group of organisms. Here, we describe Jyvaskylavirus, the first giant virus isolated from Finland. This clade B marseillevirus was found in Acanthamoeba castellanii from a composting soil sample in Jyväskylä, Central Finland. Its genome shares similarities with other marseilleviruses. Helium ion microscopy and electron microscopy of infected cells unraveled stages of the Jyvaskylavirus life cycle. We reconstructed the Jyvaskylavirus particle to 6.3 Å resolution using cryo-electron microscopy. The ~2500 Å diameter virion displays structural similarities to other Marseilleviridae giant viruses. The capsid comprises of 9240 copies of the major capsid protein, encoded by open reading frame (ORF) 184, which possesses a double jellyroll fold arranged in trimers forming pseudo-hexameric capsomers. Below the capsid shell, the internal membrane vesicle encloses the genome. Through cross-structural and -sequence comparisons with other Marseilleviridae using AI-based software in model building and prediction, we elucidated ORF142 as the penton protein, which plugs the 12 vertices of the capsid. Five additional ORFs were identified, with models predicted and fitted into densities that either cap the capsomers externally or stabilize them internally. The isolation of Jyvaskylavirus suggests that these viruses may be widespread in the boreal environment and provide structural insights extendable to other marseilleviruses.

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Metadata

Authors
Almeida, G. M. D. F., Arriaga, I., de Azevedo, B. L., Leppänen, M., Abrahão, J. S., Andreani, J., Zabeo, D., Ravantti, J. J., Abrescia, N. G., & Sundberg, L.-R.
Affiliations
  • UiT The Arctic University of Norway (NO)
  • CIC bioGUNE (ES)
  • Universidade Federal de Minas Gerais (BR)
  • Universidade Federal de Minas Gerais, Institute of Biological Sciences, Department of Microbiology
  • Aix Marseille Univ, MEPHI
  • University of Helsinki (FI)
  • University of Jyväskylä (FI)
Corresponding author
Almeida, Gabriel Magno de Freitas
ORCID
Venue
eLife
Publisher
eLife Sciences Publications, Ltd
Published
2025-03-25
Volume
13
ISSN
2050-084X (ISSN-L)
DOI
10.7554/elife.103492.3
OpenAlex
W4403484630
Cited by
0
References
3
FWCI
0.00
Type
journal article
Language
en
Open access
Licence
Locations
  • eLife (publishedVersion, cc-by)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by)
  • Jyväskylä University Digital Archive (University of Jyväskylä) (submittedVersion, cc-by)
Funders
  • Suomen Akatemia (346772)
  • Spanish Ministry of Science and Innovation (PID2021-126130OB-I00)
  • Conselho Nacional de Desenvolvimento Científico e Tecnológico
  • Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
  • Fundação de Amparo e Desenvolvimento da Pesquisa
  • Tromsø Forskningsstiftelse (ID:18_CANS_AS 311192/A65276)
Topics
  • Life Sciences › Immunology and Microbiology › Virology › Bacteriophages and microbial interactions
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Endocrinology › Legionella and Acanthamoeba research
  • Life Sciences › Immunology and Microbiology › Virology › Plant and Fungal Interactions Research
Keywords
Marseilleviridae, giant viruses, Acanthamoeba castellanii, viral genome, viral capsid structure, viral life cycle, boreal soils, Finland

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