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Cited by 77Lipid metabolism and biosynthesisLipid metabolism and disordersAquaculture Nutrition and Growth

Lipid droplet biology and evolution illuminated by the characterization of a novel perilipin in teleost fish

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Granneman, James G, Kimler, Vickie A, Zhang, Huamei, Ye, Xiangqun, Luo, Xixia, Postlethwait, John H, Thummel, Ryan·2017·eLife
DOI 10.7554/elife.21771PMC PMC5342826
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Abstract

Perilipin (PLIN) proteins constitute an ancient family important in lipid droplet (LD) formation and triglyceride metabolism. We identified an additional PLIN clade (plin6) that is unique to teleosts and can be traced to the two whole genome duplications that occurred early in vertebrate evolution. Plin6 is highly expressed in skin xanthophores, which mediate red/yellow pigmentation and trafficking, but not in tissues associated with lipid metabolism. Biochemical and immunochemical analyses demonstrate that zebrafish Plin6 protein targets the surface of pigment-containing carotenoid droplets (CD). Protein kinase A (PKA) activation, which mediates CD dispersion in xanthophores, phosphorylates Plin6 on conserved residues. Knockout of plin6 in zebrafish severely impairs the ability of CD to concentrate carotenoids and prevents tight clustering of CD within carotenoid bodies. Ultrastructural and functional analyses indicate that LD and CD are homologous structures, and that Plin6 was functionalized early in vertebrate evolution for concentrating and trafficking pigment.

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Cited by

Sorted by citations

Authors
Granneman, J. G., Kimler, V. A., Zhang, H., Ye, X., Luo, X., Postlethwait, J. H., & Thummel, R.
Affiliations
  • Wayne State University (US)
  • University of Oregon (US)
Corresponding author
  • Granneman, James G
  • Thummel, Ryan
ORCID
Venue
eLife
Publisher
eLife Sciences Publications, Ltd
Published
2017-02-28
Volume
6
ISSN
2050-084X (ISSN-L)
DOI
10.7554/elife.21771
PMC
PMC5342826
OpenAlex
W2592576198
Cited by
77
References
3
Citations by year
2026: 12 · 2025: 8 · 2024: 13 · 2023: 10 · 2022: 3 · 2021: 13 · 2020: 10 · 2019: 4 · 2018: 3 · 2017: 1
FWCI
2.41
Type
journal article
Language
en
Open access
Licence
Locations
  • eLife (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • National Institute of Diabetes and Digestive and Kidney Diseases (RO1DK076629, RO1DK62292)
  • Wayne State University (Grants Plus,Start-up funds)
  • NIH Office of the Director (5R01OD011116)
  • National Eye Institute (R21EY019401, P30EY04068)
  • Research to Prevent Blindness (Unrestricted Grant)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
  • Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Lipid metabolism and disorders
  • Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Aquaculture Nutrition and Growth
Keywords
perilipin, lipid droplets, xanthophores, teleost evolution, whole-genome duplication, protein kinase A phosphorylation, zebrafish, carotenoid content
MeSH terms
Perilipin-1, Animals, Carotenoids, Phosphorylation, Protein Processing, Post-Translational, Zebrafish, Cyclic AMP-Dependent Protein Kinases, Gene Expression Profiling, Gene Knockout Techniques, Lipid Droplets

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