The DOI matches its Crossref record; 99 of the 99 references it cites were found in a registry or an index.
Single-cell expression profiling reveals dynamic flux of cardiac stromal, vascular and immune cells in health and injury
Open accessAbstract
Besides cardiomyocytes (CM), the heart contains numerous interstitial cell types which play key roles in heart repair, regeneration and disease, including fibroblast, vascular and immune cells. However, a comprehensive understanding of this interactive cell community is lacking. We performed single-cell RNA-sequencing of the total non-CM fraction and enriched (Pdgfra-GFP+) fibroblast lineage cells from murine hearts at days 3 and 7 post-sham or myocardial infarction (MI) surgery. Clustering of >30,000 single cells identified >30 populations representing nine cell lineages, including a previously undescribed fibroblast lineage trajectory present in both sham and MI hearts leading to a uniquely activated cell state defined in part by a strong anti-WNT transcriptome signature. We also uncovered novel myofibroblast subtypes expressing either pro-fibrotic or anti-fibrotic signatures. Our data highlight non-linear dynamics in myeloid and fibroblast lineages after cardiac injury, and provide an entry point for deeper analysis of cardiac homeostasis, inflammation, fibrosis, repair and regeneration.
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Metadata
- Authors
- Farbehi, N., Patrick, R., Dorison, A., Xaymardan, M., Janbandhu, V., Wystub-Lis, K., Ho, J. W., Nordon, R. E., & Harvey, R. P.
- Venue
- eLife
- Publisher
- eLife Sciences Publications, Ltd
- Published
- 2019-03-26
- Volume
- 8
- ISSN
- 2050-084X (ISSN-L)
- OpenAlex
- W2924188812
- Cited by
- 549
- References
- 99
- FWCI
- 21.13
- Type
- journal article
- Language
- en
- Open access
- gold
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- Farbehi, N., Patrick, R., Dorison, A., Xaymardan, M., Janbandhu, V., Wystub-Lis, K., Ho, J. W., Nordon, R. E., & Harvey, R. P. (2019). Single-cell expression profiling reveals dynamic flux of cardiac stromal, vascular and immune cells in health and injury. eLife. https://doi.org/10.7554/elife.43882
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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