The transcriptional elongation rate regulates alternative polyadenylation in yeast
Open accessAbstract
Yeast cells undergoing the diauxic response show a striking upstream shift in poly(A) site utilization, with increased use of ORF-proximal poly(A) sites resulting in shorter 3’ mRNA isoforms for most genes. This altered poly(A) pattern is extremely similar to that observed in cells containing Pol II derivatives with slow elongation rates. Conversely, cells containing derivatives with fast elongation rates show a subtle downstream shift in poly(A) sites. Polyadenylation patterns of many genes are sensitive to both fast and slow elongation rates, and a global shift of poly(A) utilization is strongly linked to increased purine content of sequences flanking poly(A) sites. Pol II processivity is impaired in diauxic cells, but strains with reduced processivity and normal Pol II elongation rates have normal polyadenylation profiles. Thus, Pol II elongation speed is important for poly(A) site selection and for regulating poly(A) patterns in response to environmental conditions.
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On several genes possessing alternative poly(A) sites, a slow RNA Pol II mutant shows a switch to upstream poly(A) sites, while a fast mutant shows a slight downstream switch.
“On several genes possessing alternative poly(A) sites, a slow RNA Pol II mutant shows a switch to the use of upstream poly(A) sites (Pinto et al, 2011; Liu et al, 2017; Geisberg et al, 2020; Yague‐Sanz et al, 2020), while a fast RNA Pol II mutant shows a slight downstream switch in poly(A) site usage (Geisberg et al, 2020).”
From RNA polymerase II speed: a key player in controlling and adapting transcriptome composition (2021) · cites this paper as Geisberg et al, 2020
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References 59 · Cited by 45
- Authors
- Geisberg, J. V., Moqtaderi, Z., & Struhl, K.
- Affiliations
- Harvard University (US)
- Corresponding author
- Struhl, Kevin
- Venue
- eLife
- Publisher
- eLife Sciences Publications, Ltd
- Published
- 2020-08-26
- Volume
- 9
- ISSN
- 2050-084X (ISSN-L)
- PMC
- PMC7532003
- OpenAlex
- W3080460569
- Cited by
- 45
- References
- 59
- Citations by year
- 2026: 6 · 2025: 6 · 2024: 11 · 2023: 9 · 2022: 8 · 2021: 4 · 2020: 1
- FWCI
- 1.78
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- eLife (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- National Institutes of Health (GM 30186, GM 131801)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
- Keywords
- alternative polyadenylation, RNA polymerase II processivity, poly(A) site selection, diauxic shift, yeast, mRNA isoforms, purine content
- MeSH terms
- Poly A, RNA Polymerase II, Saccharomyces cerevisiae, Polyadenylation, Saccharomyces cerevisiae Proteins, Transcription Elongation, Genetic
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