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Cited by 31Neurobiology and Insect Physiology ResearchOlfactory and Sensory Function StudiesEvolution and Genetic Dynamics

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Molecular reconstruction of recurrent evolutionary switching in olfactory receptor specificity

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Prieto-Godino, Lucia L, Schmidt, Hayden R, Benton, Richard·2021·eLife
DOI 10.7554/elife.69732PMC PMC8575457

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Abstract

Olfactory receptor repertoires exhibit remarkable functional diversity, but how these proteins have evolved is poorly understood. Through analysis of extant and ancestrally reconstructed drosophilid olfactory receptors from the Ionotropic receptor (Ir) family, we investigated evolution of two organic acid-sensing receptors, Ir75a and Ir75b. Despite their low amino acid identity, we identify a common ‘hotspot’ in their ligand-binding pocket that has a major effect on changing the specificity of both Irs, as well as at least two distinct functional transitions in Ir75a during evolution. Moreover, we show that odor specificity is refined by changes in additional, receptor-specific sites, including those outside the ligand-binding pocket. Our work reveals how a core, common determinant of ligand-tuning acts within epistatic and allosteric networks of substitutions to lead to functional evolution of olfactory receptors.

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Metadata

Authors
Prieto-Godino, L. L., Schmidt, H. R., & Benton, R.
Affiliations
  • The Francis Crick Institute (GB)
  • University of Lausanne (CH)
Corresponding author
  • Prieto-Godino, Lucia L
  • Benton, Richard
ORCID
Venue
eLife
Publisher
eLife Sciences Publications, Ltd
Published
2021-10-22
Volume
10
ISSN
2050-084X (ISSN-L)
DOI
10.7554/elife.69732
PMC
PMC8575457
OpenAlex
W3206610810
Cited by
31
References
1
Citations by year
2026: 4 · 2025: 9 · 2024: 5 · 2023: 7 · 2022: 5 · 2021: 1
FWCI
2.07
Type
journal article
Language
en
Open access
Licence
Locations
  • eLife (publishedVersion, cc-by)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • SERVAL (Université de Lausanne) (publishedVersion, cc-by)
  • IRIS (submittedVersion, cc-by)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • Figshare (submittedVersion, cc-by)
  • The Francis Crick Institute (cc-by)
Funders
  • EMBO (ALTF 940-2019)
  • Helen Hay Whitney Foundation
  • European Research Council (802531, 615094, 833548)
  • Cancer Research UK (FC001594)
  • Medical Research Council (FC001594)
  • Wellcome Trust (FC001594)
  • Swiss National Science Foundation (20NA21_143082)
  • Human Frontier Science Program (RGY0073/2011)
Topics
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurobiology and Insect Physiology Research
  • Life Sciences › Neuroscience › Sensory Systems › Olfactory and Sensory Function Studies
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Evolution and Genetic Dynamics
Keywords
olfactory receptor evolution, ligand-gated ion channels, binding pocket, ancestral sequence reconstruction, epistasis, allosteric networks, Drosophila, functional transitions
MeSH terms
Animals, Drosophila melanogaster, Odorants, Olfactory Receptor Neurons, Receptors, Odorant, Evolution, Molecular

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