CoChat
Sign Up
PreprintCited by 0Developmental Biology and Gene RegulationWnt/β-catenin signaling in development and cancerGenomics and Chromatin Dynamics

The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.

Dynamic compartmentalization of the pro-invasive transcription factor NHR-67 reveals a role for Groucho in regulating a proliferative-invasive cellular switch in C. elegans

Open access
Medwig-Kinney, Taylor N., Kinney, Brian A., Martinez, Michael A. Q., Yee, Callista, Sirota, Sydney S., Mullarkey, Angelina A., Somineni, Neha, Hippler, Justin, Zhang, Wan, Shen, Kang, Hammell, Christopher M., Pani, Ariel M., Matus, David Q.·2023·eLife
DOI 10.7554/elife.84355.2

Preprint — published as 10.7554/elife.84355.3 · Paper page

Open this paper in CoChat — free account

Abstract

Abstract A growing body of evidence suggests that cell division and basement membrane invasion are mutually exclusive cellular behaviors. How cells switch between proliferative and invasive states is not well understood. Here, we investigated this dichotomy in vivo by examining two cell types in the developing Caenorhabditis elegans somatic gonad that derive from equipotent progenitors, but exhibit distinct cell behaviors: the post-mitotic, invasive anchor cell and the neighboring proliferative, non-invasive ventral uterine (VU) cells. We show that the fates of these cells post-specification are more plastic than previously appreciated and that levels of NHR-67 are important for discriminating between invasive and proliferative behavior. Transcription of nhr-67 is downregulated following post-translational degradation of its direct upstream regulator, HLH-2 (E/Daughterless) in VU cells. In the nuclei of VU cells, residual NHR-67 protein is compartmentalized into discrete punctae that are dynamic over the cell cycle and exhibit liquid-like properties. By screening for proteins that colocalize with NHR-67 punctae, we identified new regulators of uterine cell fate maintenance: homologs of the transcriptional co-repressor Groucho (UNC-37 and LSY-22), as well as the TCF/LEF homolog POP-1. We propose a model in which association of NHR-67 with the Groucho/TCF complex suppresses the default invasive state in non-invasive cells, which complements transcriptional regulation to add robustness to the proliferative-invasive cellular switch in vivo.

Overview

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.7554/elife.84355.2

Cites

2 of 2 references verified0 citation errors · 0 not checkable

Statistics

Verification

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Citations

Cites

Flagged first, then by citations

Research works printed but not linked: 117

  1. 2020, Visualizing the metazoan proliferation-quiescence decision in vivo, Elife, 9, 1, 10.7554/eLife.63265 · DOI
  2. 2021, An expanded auxin-inducible degron toolkit for Caenorhabditis elegans, Genetics, 10.1093/genetics/iyab006 · DOI
  3. 2019, HLH-2/E2A Expression Links Stochastic and Deterministic Elements of a Cell Fate Decision during C. elegans Gonadogenesis, Curr Biol, 29, 1, 10.1016/j.cub.2019.07.062 · DOI
  4. 2022, unc-37/Groucho and lsy-22/AES repress Wnt target genes in C. elegans asymmetric cell divisions
  5. 2022, Influences of HLH-2 stability on anchor cell fate specification during Caenorhabditis elegans gonadogenesis, G3|58; Genes|Genomes|Genetics
  6. 2018, A tissue-specific enhancer of the C. elegans nhr-67/tailless gene drives coordinated expression in uterine stem cells and the differentiated anchor cell, Gene Expr Patterns, 30, 71, 10.1016/j.gep.2018.10.003 · DOI
  7. 2018, Transcription Factors Activate Genes through the Phase-Separation Capacity of Their Activation Domains, Cell, 175, 1842, 10.1016/j.cell.2018.10.042 · DOI · Paper page
  8. 2006, A guided tour into subcellular colocalization analysis in light microscopy, J Microsc, 224, 213, 10.1111/j.1365-2818.2006.01706.x · DOI
  9. 2008, A Protein Domain-Based Interactome Network for C. elegans Early Embryogenesis, Cell, 134, 534, 10.1016/j.cell.2008.07.009 · DOI
  10. 2009, Germline P Granules Are Liquid Droplets That Localize by Controlled Dissolution/Condensation, Science (80-), 324, 1729, 10.1126/science.1172046 · DOI
  11. 1974, The genetics of Caenorabditis elegans, Genetics, 77, 71, 10.1016/S0047-2484(78)80101-8 · DOI
  12. 2019, Reprogramming : identifying the mechanisms that safeguard cell identity, Development, 146, 1, 10.1242/dev.182170 · DOI
  13. 2001, A POP-1 repressor complex restricts inappropriate cell type-specific gene transcription during Caenorhabditis elegans embryogenesis, EMBO J, 20, 7197, 10.1093/emboj/20.24.7197 · DOI
  14. 2018, Mediator and RNA polymerase II clusters associate in transcription-dependent condensates, Science (80-), 361, 412, 10.1126/science.aar4199 · DOI · Paper page
  15. 2018, Imaging dynamic and selective low-complexity domain interactions that control gene transcription, Science (80-), 361, 10.1126/science.aar2555 · DOI · Paper page
  16. 1992, A Power Primer, Psychol Bull, 112, 155, 10.1038/141613a0 · DOI
  17. 1987, Expression of a single transfected cDNA converts fibroblasts to myoblasts, Cell, 51, 987, 10.1016/0092-8674(87)90585-X · DOI
  18. 2016, CRISPR-based methods for caenorhabditis elegans genome engineering, Genetics, 202, 885, 10.1534/genetics.115.182162 · DOI
  19. 2015, Streamlined genome engineering with a self-excising drug selection cassette, Genetics, 200, 1035, 10.1534/genetics.115.178335 · DOI
  20. 2010, The Groucho ortholog UNC-37 interacts with the short Groucho-like protein LSY-22 to control developmental decisions in C. elegans, Development, 137, 1799, 10.1242/dev.046219 · DOI
  21. 2013, Improved Mos1-mediated transgenesis in C. elegans, Nat Meth, 9, 117, 10.1038/mp.2011.182.doi · DOI
  22. 2005, The myogenic potency of HLH-1 reveals wide-spread developmental plasticity in early C. elegans embryos, Development, 132, 1795, 10.1242/dev.01774 · DOI
  23. 2020, Melting dsDNA donor molecules greatly improves precision genome editing in caenorhabditis elegans, Genetics, 216, 643, 10.1534/genetics.120.303564 · DOI
  24. 2001, Activation of Hypodermal Differentiation in the Caenorhabditis elegans Embryo by GATA Transcription Factors ELT-1 and ELT-3, Mol Cell Biol, 21, 2533, 10.1128/mcb.21.7.2533-2544.2001 · DOI
  25. 2020, Tissue-Specific Transcription Footprinting Using RNA, Genetics, 216, 931, 10.1534/genetics.120.303774 · DOI
  26. 2008, Opposing Wnt pathways orient cell polarity during organogenesis, Cell, 134, 646, 10.1016/j.cell.2008.06.026.Opposing · DOI
  27. 1983, The lin-12 locus specifies cell fates in Caenorhabditis elegans, Cell, 34, 435, 10.1016/0092-8674(83)90377-X · DOI
  28. 2019, MRG-1 / MRG15 Is a Barrier for Germ Cell to Neuron reprogramming, Genetics, 211, 121, 10.1534/genetics.118.301674 · DOI
  29. 2022, Hallmarks of Cancer: New Dimensions, Cancer Discov, 31, 10.1158/2159-8290.CD-21-1059 · DOI · Paper page
  30. 2000, The hallmarks of cancer, Cell, 100, 57, 10.1007/s00262-010-0968-0 · DOI
  31. 2020, WormBase: A Modern Model Organism Information Resource, Nucleic Acids Res, 48, D762, 10.1093/nar/gkz920 · DOI
  32. 2021, An engineered, orthogonal auxin analog/AtTIR1(F79G) pairing improves both specificity and efficacy of the auxin degradation system in Caenorhabditis elegans, Genetics iyab, 174, 10.1093/genetics/iyab174 · DOI
  33. 1998, . pha-4, an HNF-3 homolog, specifies pharyngeal organ identity in Caenorhabditis elegans, Genes Dev, 12, 1947, 10.1101/gad.12.13.1947 · DOI
  34. 2021, Improved CRISPR / Cas9 knock-in efficiency via the self-excising cassette (SEC) selection method in C . elegans, Micropublication Biol
  35. 2022, Fixation can change the appearance of phase separation in living cells, Elife, 11, 10.7554/eLife.79903 · DOI
  36. 2008, A Caenorhabditis elegans model for epithelial-neuronal transdifferentiation, Proc Natl Acad Sci, 105, 3790, 10.1073/pnas.0712159105 · DOI
  37. 2015, Cell cycle and p53 gate the direct conversion of human fibroblasts to dopaminergic neurons, Nat Commun, 6, 10.1038/ncomms10100 · DOI
  38. 1994, Control of type-D GABAergic neuron differentiation by C. elegans UNC-30 homeodomain protein, Nature, 372, 780, 10.1038/372780a0 · DOI
  39. 2012, Members of the NODE (Nanog and Oct4-associated deacetylase) complex and SOX-2 promote the initiation of a natural cellular reprogramming event in vivo, Proc Natl Acad Sci U S A, 109, 6596, 10.1073/pnas.1117031109 · DOI
  40. 2003, Post-transcriptional regulation of the E/Daughterless ortholog HLH-2, negative feedback, and birth order bias during the AC/VU decision in C. elegans, Genes Dev, 17, 3100, 10.1101/gad.1160803 · DOI
  41. 2022, Targeted DamID in C . elegans reveals a direct role for LIN-22 and NHR-25 in antagonizing the epidermal stem cell fate, Sci Adv, 8, eabk3141, 10.1126/sciadv.abk3141 · DOI
  42. 2020, Comprehensive Endogenous Tagging of Basement Membrane Components Reveals Dynamic Movement within the Matrix Scaffolding, Dev Cell, 54, 60, 10.1016/j.devcel.2020.05.022 · DOI
  43. 2017, Live-cell confocal microscopy and quantitative 4D image analysis of anchor-cell invasion through the basement membrane in Caenorhabditis elegans, Nat Protoc, 12, 2081, 10.1038/nprot.2017.093 · DOI
  44. 2007, PHA-4/FoxA cooperates with TAM-1/TRIM to regulate cell fate restriction in the C. elegans foregut, Dev Biol, 303, 611, 10.1016/j.ydbio.2006.11.042 · DOI
  45. 1979, The postembryonic cell lineages of the hermaphrodite and male gonads in Caenorhabditis elegans, Dev Biol, 70, 396, 10.1016/0012-1606(79)90035-6 · DOI
  46. 2017, Divide or Conquer: Cell Cycle Regulation of Invasive Behavior, Trends Cell Biol, 10.1016/j.tcb.2016.08.003 · DOI
  47. 2018, FACT Sets a Barrier for Cell Fate Reprogramming in Caenorhabditis elegans and Human Cells, Dev Cell, 46, 611, 10.1016/j.devcel.2018.07.006 · DOI
  48. 2021, Sustained expression of unc-4 homeobox gene and unc-37/groucho in postmitotic neurons specifies the spatial organization of the cholinergic synapses in c. Elegans, Elife, 10, 1, 10.7554/eLife.66011 · DOI
  49. 2017, Liquid droplet formation by HP1α suggests a role for phase separation in heterochromatin, Nature, 547, 236, 10.1038/nature22822 · DOI
  50. 2019, Transcription factor autoregulation is required for acquisition and maintenance of neuronal identity, Development, 146, 10.1242/dev.177378 · DOI
  51. 2004, A Map of the Interactome Network of the Metazoan C. elegans, Science (80-), 303, 540, 10.1126/science.1091403 · DOI
  52. 2020, EVI1 in leukemia and solid tumors, Cancers (Basel, 12, 1, 10.3390/cancers12092667 · DOI
  53. 2021, Enhancer-promoter communication: hubs or loops?, Curr Opin Genet Dev, 67, 5, 10.1016/j.gde.2020.10.001 · DOI
  54. 2021, A 4D single-cell protein atlas of transcription factors delineates spatiotemporal patterning during embryogenesis, Nat Methods, 18, 893, 10.1038/s41592-021-01216-1 · DOI
  55. 2002, Dynamics of a developmental switch: Recursive intracellular and intranuclear redistribution of Caenorhabditis elegans POP-1 parallels Wnt-inhibited transcriptional repression, Dev Biol, 248, 128, 10.1006/dbio.2002.0721 · DOI
  56. 2020, Auxin-mediated Protein Degradation in Caenorhabditis elegans, Bio-Protocol, 10, 1, 10.21769/bioprotoc.3589 · DOI
  57. 2020, Rapid Degradation of Caenorhabditis elegans Proteins at Single-Cell Resolution with a Synthetic Auxin, G3 Genes, Genomes, Genet, 10, 267, 10.1534/g3.119.400781 · DOI
  58. 2010, In vivo identification of regulators of cell invasion across basement membranes, Sci Signal, 3, ra35, 10.1126/scisignal.2000654 · DOI
  59. 2015, Invasive Cell Fate Requires G1 Cell-Cycle Arrest and Histone Deacetylase-Mediated Changes in Gene Expression, Dev Cell, 35, 162, 10.1016/j.devcel.2015.10.002 · DOI
  60. 1993, Two Types of Sites Required for Meiotic Chromosome Pairing in Caenorhabditis elegans, Genetics, 134, 749, 10.1093/genetics/134.3.749 · DOI
  61. 2021, Deletion of a putative HDA-1 binding site in the hlh-2 promoter eliminates expression in C. elegans dorsal uterine cells, Micropublication Biol, 1
  62. 2022, An in vivo toolkit to visualize endogenous LAG-2/Delta and LIN-12/Notch signaling in C. elegans, microPublication Biol
  63. 2020, A developmental gene regulatory network for C. elegans anchor cell invasion, Development, 147, dev185850, 10.1101/691337 · DOI
  64. 2017, Breaking down barriers: the evolution of cell invasion, Curr Opin Genet Dev, 47, 33, 10.1016/j.gde.2017.08.003 · DOI
  65. 2005, The Fos family of transcription factors and their role in tumourigenesis, Eur J Cancer, 41, 2449, 10.1016/j.ejca.2005.08.008 · DOI · Paper page
  66. 2021, A Proliferative to Invasive Switch is Mediated by srGAP1 Downregulation Through the Activation of TGFβ2 Signaling, SSRN Electron J, 40, 111358, 10.1016/j.celrep.2022.111358 · DOI
  67. 2021, TLX, an Orphan Nuclear Receptor with Emerging Roles in Physiology and Disease, Endocrinol (United States, 162, 1, 10.1210/endocr/bqab184 · DOI
  68. 2022, A new toolkit to visualize and perturb endogenous LIN-12/Notch signaling, Micropublication Biol
  69. 2017, Coordinated control of terminal differentiation and restriction of cellular plasticity, Elife, 6, 1, 10.7554/elife.24100 · DOI
  70. 2012, Removal of Polycomb Repressive Complex 2 Makes C. elegans Germ Cells Susceptible to Direct Conversion into Specific Somatic Cell Types, Cell Rep, 2, 1178, 10.1016/j.celrep.2012.09.020 · DOI
  71. 2006, Precise positions of Phoebe determined with CCD image-overlapping calibration, Mon Not R Astron Soc, 366, 208, 10.1186/1471-2105-7-208 · DOI
  72. 1997, The Groucho-like transcription factor UNC-37 functions with the neural specificity gene unc-4 to govern motor neuron identity in C. elegans, Development, 124, 1699, 10.1242/dev.124.9.1699 · DOI
  73. 2007, Reciprocal asymmetry of SYS-1/β-catenin and POP-1/TCF controls asymmetric divisions in Caenorhabditis elegans, Proc Natl Acad Sci U S A, 104, 3231, 10.1073/pnas.0611507104 · DOI
  74. 2009, A New Look at TCF and β-Catenin through the Lens of a Divergent C. elegans Wnt Pathway, Dev Cell, 17, 27, 10.1016/j.devcel.2009.07.002 · DOI
  75. 2012, Basic Caenorhabditis elegans Methods: Synchronization and Observation, J Vis Exp, 1, 10.3791/4019 · DOI
  76. 2019, A gel phase promotes condensation of liquid P granules in Caenorhabditis elegans embryos, Nat Struct Mol Biol, 26
  77. 2001, The Caenorhabditis elegans gene lin-26 can trigger epithelial differentiation without conferring tissue specificity, Dev Biol, 235, 410, 10.1006/dbio.2001.0294 · DOI
  78. 2019, Restriction of Cellular Plasticity of Differentiated Cells Mediated by Chromatin Modifiers, Transcription Factors and Protein Kinases. G3|58; Genes|Genomes|Genetics, 9, 2287, 10.1534/g3.119.400328 · DOI
  79. 2005, A compendium of Caenorhabditis elegans regulatory transcription factors: A resource for mapping transcription regulatory networks, Genome Biol, 6, 10.1186/gb-2005-6-13-r110 · DOI
  80. 2018, High-efficiency yeast transformation, Cold Spring Harb Protoc, 2018, 563, 10.1101/pdb.prot094995 · DOI
  81. 2011, Direct in vivo cellular reprogramming involves transition through discrete, non-pluripotent steps, J Cell Sci, 124, e1, 10.1242/jcs.090043 · DOI
  82. 2016, Transorganogenesis and transdifferentiation in C. elegans are dependent on differentiated cell identity, Dev Biol, 420, 136, 10.1016/j.ydbio.2016.09.020 · DOI
  83. 2013, Transdifferentiation and remodeling of post-embryonic C. elegans cells by a single transcription factor, Development, 140, 4844, 10.1242/dev.103010 · DOI
  84. 2022, A natural transdifferentiation event involving mitosis is empowered by integrating signaling inputs with conserved plasticity factors, Cell Rep, 40, 111365, 10.1016/j.celrep.2022.111365 · DOI
  85. 2019, Developmental Plasticity and Cellular Reprogramming, Genetics, 213, 723, 10.1534/genetics.119.302333 · DOI
  86. 2004, Toward improving Caenorhabditis elegans phenome mapping with an ORFeome-based RNAi library, Genome Res, 14, 2162, 10.1101/gr.2505604 · DOI
  87. 2018, Coactivator condensation at super-enhancers links phase separation and gene control, Science (80-), 361, 10.1126/science.aar3958 · DOI · Paper page
  88. 2015, Influences of LIN-12/Notch and POP-1/TCF on the Robustness of Ventral Uterine Cell Fate Specification in Caenorhabditis elegans Gonadogenesis, G3|58; Genes|Genomes|Genetics, 5, 2775, 10.1534/g3.115.022608 · DOI
  89. 2015, Dimerization-driven degradation of C. elegans and human E proteins, Genes Dev, 29, 1356, 10.1101/gad.261917.115 · DOI
  90. 2017, A bHLH Code for Sexually Dimorphic Form and Function of the C. elegans Somatic Gonad, Curr Biol, 27, 1853, 10.1016/j.cub.2017.05.059 · DOI
  91. 2012, Fiji: an open-source platform for biological-image analysis, Nat Methods, 9, 676, 10.1038/nmeth.2019 · DOI · Paper page
  92. 1989, Cell autonomy of lin-12 function in a cell fate decision in C. elegans, Cell, 57, 1237, 10.1016/0092-8674(89)90060-3 · DOI
  93. 1990, Cell-cell interactions prevent a potential inductive interaction between soma and germline in C. elegans, Cell, 61, 939, 10.1016/0092-8674(90)90060-R · DOI
  94. 2003, Anchor cell invasion into the vulval epithelium in C. elegans, Dev Cell, 5, 21, 10.1016/S1534-5807(03)00168-0 · DOI
  95. 2005, C. elegans TCF protein, POP-1, converts from repressor to activator as a result of Wnt-induced lowering of nuclear levels, Dev Biol, 285, 584, 10.1016/j.ydbio.2005.07.008 · DOI
  96. 2002, POP-1 controls axis formation during early gonadogenesis in C. elegans, Development, 453, 443
  97. 2004, The sys-1 and sys-3 Genes Cooperate with Wnt Signaling to Establish the Proximal-Distal Axis of the Caenorhabditis elegans Gonad, Genetics, 166, 171, 10.1534/genetics.166.1.171 · DOI
  98. 2009, Empirically controlled mapping of the Caenorhabditis elegans protein-protein interactome network, Nat Methods, 6, 47, 10.1038/nmeth.1279 · DOI
  99. 2022, The SWI/SNF chromatin remodeling assemblies BAF and PBAF differentially regulate cell cycle exit and cellular invasion in vivo, PLOS Genetics, 10.1371/journal.pgen.1009981 · DOI
  100. 2013, The proliferation-quiescence decision is controlled by a bifurcation in CDK2 activity at mitotic exit, Cell, 155, 369, 10.1016/j.cell.2013.08.062 · DOI
  101. 2017, Phase separation drives heterochromatin domain formation, Nature, 547, 241, 10.1038/nature22989 · DOI
  102. 2018, Highly efficient RNAi and Cas9-based auto-cloning systems for C. elegans research, Nucleic Acids Res, 46, e105, 10.1093/nar/gky516 · DOI
  103. 1977, Post-embryonic cell lineages of the nematode, Caenorhabditis elegans, Dev Biol, 56, 110, 10.1016/0012-1606(77)90158-0 · DOI
  104. 2021, Properties of repression condensates in living Ciona embryos, Nat Commun, 12, 1, 10.1038/s41467-021-21606-5 · DOI
  105. 2011, Direct Conversion of C. elegans Germ Cells into Specific Neuron Types, Science (80-), 331, 304, 10.1126/science.1199082 · DOI
  106. 2019, C . elegans Runx / CBFβ suppresses POP-1 (TCF) to convert asymmetric to proliferative division of stem cell-like seam cells, Development, 146, 1, 10.1242/dev.180034 · DOI
  107. 2022, AlphaFold Protein Structure Database: massively expanding the structural coverage of protein-sequence space with high-accuracy models, Nucleic Acids Res, 50, D439, 10.1093/nar/gkab1061 · DOI · Paper page
  108. 2011, The tailless ortholog nhr-67 functions in the development of the C. elegans ventral uterus, Dev Biol, 356, 516, 10.1016/j.ydbio.2011.06.007 · DOI
  109. 2015, E Proteins and ID Proteins: Helix-Loop-Helix Partners in Development and Disease, Dev Cell, 35, 269, 10.1016/j.devcel.2015.10.019 · DOI
  110. 2017, A toolkit for GFP-mediated tissue-specific protein degradation in C. elegans, Development, 144, 2694, 10.1242/dev.150094 · DOI · Paper page
  111. 2016, ggplot2: Elegant Graphics for Data Analysis
  112. 1994, Reciprocal changes in expression of the receptor lin-12 and its ligand lag-2 prior to commitment in a C. elegans cell fate decision, Cell, 79, 1187, 10.1016/0092-8674(94)90010-8 · DOI
  113. 2015, Quantitative Differences in Nuclear β-catenin and TCF Pattern Embryonic Cells in C. elegans, PLoS Genet, 11, 10.1371/journal.pgen.1005585 · DOI
  114. 2015, The auxin-inducible degradation (AID) system enables versatile conditional protein depletion in C. elegans, Development, 142, 4374, 10.1242/dev.129635 · DOI
  115. 2006, Genome-Wide Prediction of C. elegans Genetic Interactions, Science (80-), 311, 1481, 10.1126/science.1123287 · DOI
  116. 1998, Reprogramming of early embryonic blastomeres into endodermal progenitors by a Caenorhabditis elegans GATA factor, Genes Dev, 12, 3809, 10.1101/gad.12.24.3809 · DOI
  117. 2014, Sequential histone-modifying activities determine the robustness of transdifferentiation, Science (80-), 345, 826, 10.1126/science.1255885 · DOI

Metadata

Authors
Medwig-Kinney, T. N., Kinney, B. A., Martinez, M. A. Q., Yee, C., Sirota, S. S., Mullarkey, A. A., Somineni, N., Hippler, J., Zhang, W., Shen, K., Hammell, C. M., Pani, A. M., & Matus, D. Q.
Affiliations
  • Stony Brook University (US)
  • Cold Spring Harbor Laboratory (US)
  • Howard Hughes Medical Institute (US)
  • University of Virginia (US)
ORCID
Venue
eLife
Publisher
eLife Sciences Publications, Ltd
Published
2023-09-26
ISSN
2050-084X (ISSN-L)
DOI
10.7554/elife.84355.2
OpenAlex
W4387048210
Cited by
0
References
2
Type
posted content
Language
en
Open access
Licence
Locations
eLife (acceptedVersion, cc-by)
Funders
  • National Institutes of Health (R01GM121597, F31HD100091, F30CA257383, R01GM117406, R35GM142880)
  • Damon Runyon Cancer Research Foundation (DRR-47-17)
  • Stony Brook University (Presidential Critical Research Funds)
  • Human Frontier Science Program (LTF000127/2016-L)
  • Howard Hughes Medical Institute (Investigator)
  • National Science Foundation (2217560)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Wnt/β-catenin signaling in development and cancer
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
Keywords
Groucho, POP-1, anchor cell, basement membrane invasion, corepressors, nuclear compartmentalization, cell fate maintenance, Caenorhabditis elegans

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review