The zebrafish mutant dreammist implicates sodium homeostasis in sleep regulation
Open accessPublished version of a preprint
Abstract
Sleep is a nearly universal feature of animal behaviour, yet many of the molecular, genetic, and neuronal substrates that orchestrate sleep/wake transitions lie undiscovered. Employing a viral insertion sleep screen in larval zebrafish, we identified a novel gene, dreammist ( dmist ), whose loss results in behavioural hyperactivity and reduced sleep at night. The neuronally expressed dmist gene is conserved across vertebrates and encodes a small single-pass transmembrane protein that is structurally similar to the Na + ,K + -ATPase regulator, FXYD1/Phospholemman. Disruption of either fxyd1 or atp1a3a , a Na + ,K + -ATPase alpha-3 subunit associated with several heritable movement disorders in humans, led to decreased night-time sleep. Since atpa1a3a and dmist mutants have elevated intracellular Na + levels and non-additive effects on sleep amount at night, we propose that Dmist-dependent enhancement of Na + pump function modulates neuronal excitability to maintain normal sleep behaviour.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
Highly accurate protein structure prediction with AlphaFold
Jumper, J., Evans, R., Pritzel, A., Green, T., Figurnov, M., Ronneberger, O., Tunyasuvunakool, K., Bates, R., Žídek, A., Potapenko, A., et al. (2021) · Nature · doi:10.1038/s41586-021-03819-2 · Cited by 43,764 · see citing works
Zhang, Y., Chen, K., Sloan, S. A., Bennett, M. L., Scholze, A. R., O'Keeffe, S., Phatnani, H. P., Guarnieri, P., Caneda, C., Ruderisch, N., et al. (2014) · The Journal of Neuroscience · doi:10.1523/jneurosci.1860-14.2014 · Cited by 4,875 · see citing works
Genetic studies of accelerometer-based sleep measures yield new insights into human sleep behaviour
Jones, S. E., van Hees, V. T., Mazzotti, D. R., Marques-Vidal, P., Sabia, S., van der Spek, A., Dashti, H. S., Engmann, J., Kocevska, D., Tyrrell, J., et al. (2019) · Nature Communications · doi:10.1038/s41467-019-09576-1 · Cited by 300 · see citing works
A Zebrafish Genetic Screen Identifies Neuromedin U as a Regulator of Sleep/Wake States
Chiu, C. N., Rihel, J., Lee, D. A., Singh, C., Mosser, E. A., Chen, S., Sapin, V., Pham, U., Engle, J., Niles, B. J., et al. (2016) · Neuron · doi:10.1016/j.neuron.2016.01.007 · Cited by 101 · see citing works
Self-citation
Flagged first, then by citations
- Authors
- Barlow, I. L., Mackay, E., Wheater, E., Goel, A., Lim, S., Zimmerman, S., Woods, I., Prober, D. A., & Rihel, J.
- Affiliations
- University College London (GB)
- Harvard University (US)
- Ithaca College (US)
- California Institute of Technology (US)
- Corresponding author
- Rihel, Jason
- ORCID
- Venue
- eLife
- Publisher
- eLife Sciences Publications, Ltd
- Published
- 2023-08-07
- Volume
- 12
- ISSN
- 2050-084X (ISSN-L)
- PMC
- PMC10406431
- OpenAlex
- W4375949141
- Cited by
- 5
- References
- 4
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 2
- FWCI
- 0.75
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- eLife (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- Wellcome Trust (217150/Z/19/Z)
- European Research Council (282027)
- National Institutes of Health (R35 NS122172)
- Topics
- Life Sciences › Neuroscience › Cognitive Neuroscience › Sleep and Wakefulness Research
- Life Sciences › Neuroscience › Endocrine and Autonomic Systems › Circadian rhythm and melatonin
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Zebrafish Biomedical Research Applications
- Keywords
- sleep regulation, sodium homeostasis, FXYD1, Na+/K+-ATPase, intracellular sodium, neuronal excitability, sleep-wake transition, hyperactivity, zebrafish larvae, transmembrane proteins
- MeSH terms
- Sodium-Potassium-Exchanging ATPase, Animals, Homeostasis, Humans, Phosphoproteins, Sleep, Sodium, Zebrafish
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
