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Chemical Properties and DPPH Radical Scavenging Ability of Sword Bean (Canavalia gladiata) Extract
Open accessAbstract
본 연구에서 추출 용매의 선택은 작두콩과 대두, 서리태의 추출 수율과 실험의 용이성 등을 고려하여 메탄올을 사용하여 실험을 진행하였다. 작두콩의 화학적 특성을 알아보기 위해 일반성분 중 수분, 회분, 조단백질, 조지방 실험을 하였으며, 이 중 조지방에서 서리태, 대두와는 다른 작두콩만의 특징을 찾을 수 있었다. 작두콩의 조지방 함량은 1.2%로 서리태와 대두의 조지방 함량 16.1, 16.5%와 비교해 볼 때 매우 낮음을 알 수 있었다. 이 낮은 조지방 함량으로 인해 비극성 유기용매 헥산이나 에틸아세테이트 등에서 추출 수율이 서리태나 대두에 비해 낮게 나타났다. 구성아미노산은 함량과 구성비율을 실험하였다. 그 결과 Histidine의 구성비율이 서리태(2.9%)나 대두(3.0%)와 비교해 볼때 9.2%로 상대적으로 높은 구성비율을 나타냈다. 콩에서 대표적인 항산화활성 물질로 알려진 이소플라본은 작두콩에서 검출되지 않았다. 작두콩과 대두, 서리태의 비타민 실험결과는 모두 비타민 C의 함량이 비타민 A의 함량보다 높게 검출되었다. 무기질 함량 실험에서는 작두콩과 대두, 서리태에서 10가지 무기질 중 칼륨, 칼슘, 마그네슘의 함량이 높게 나타났다. 총 폴리페놀은 대두 1,165.7 mg/100 g, 서리태 1,298.6 mg/100 g, 작두콩 1,152.0 mg/100 g으로 서리태 보다는 적지만 대두와는 유의적 차이를 보이지 않았다. 총 플라보노이드는 대두 71.8 mg/100 g, 서리태 97.5 mg/100 g보다 작두콩에서 유의적으로 높게(493.2 mg/100 g) 나타났다. DPPH 라디칼 소거활성 결과를 토대로 $SC_{50}$ 값을 구한 결과 ${\alpha}$ -tocopherol(8.3 ${\mu}g/mL$ )과 대등한 13.1 ${\mu}g/mL$ 로 항산화활성이 높게 나타났으며, 농도 의존적으로 활성이 증가하고 있음을 알수 있었다. 이상의 결과 같은 콩과의 식물이지만 대두나 서리태와는 다른 화학적 특성을 가지고 있고 높은 항산화활성을 갖는 작두콩의 식품이나 건강기능식품으로서의 활용가치를 확인할 수 있었고, 항산화활성 물질의 분리 등 더 많은 연구가 필요할 것으로 생각된다. We investigated the chemical properties and antioxidant activities of sword bean (SWB) and compared it to soybean (SB) and black soybean (seoritae, BSB). The value of vitamin C, vitamin A, crude fat, and crude protein in SWB was 25.5, 0.37 mg/kg, 1.2, and 25.6%, respectively. The crude fat content (1.2%) in SWB was very low in comparison with those of SB (16.5%) and BSB (16.1%). In 16 free amino acids investigated, the histidine content (9.2%) was high in SWB, followed by SB (3.0%) and BSB (2.9%). Total flavonoid content of SWB (493.2 mg/100 g) was significantly higher than those of SB (71.8 mg/100 g) and BSB (97.5 mg/100 g). Total polyphenol content of SWB (1,152.0 mg/100 g) was not significantly different from that of SB (1,165.7 mg/100 g) but lower than that of BSB (1,298.6 mg/100 g). DPPH radical scavenging activity ( $SC_{50}$ , 50% scavenging concentration) of SWB was 13.1 ${\mu}g/mL$ , whereas that of positive control ( ${\alpha}$ -tocopherol) was 8.3 ${\mu}g/mL$ .
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“Additionally, the study lacks information on potential confounding variables such as participants' skincare routine or sun exposure history.”
From Cosmeceuticals in photoaging: A review. (2024) · cites this paper as [53]
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“The authors conducted a randomized controlled trial on 60 participants and assessed skin elasticity, hydration, and wrinkles before and after the intervention.”
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Antioxidant Activities of Black and Yellow Soybeans against Low Density Lipoprotein Oxidation
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CHARACTERISTICS OF YOGURT PREPARED WITH JINPUM BEAN AND SWORD BEAN (CANAVALIN GLADIATA)
Joo, S.-H., Choi, K.-S., & Kim, K. S. (2001) · Cited by 15
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Cosmeceuticals in photoaging: A review.
Chan, L. K. W., Lee, K. W. A., Lee, C. H., Lam, K. W. P., Lee, K. F. V., Wu, R., Wan, J., Shivananjappa, S., Sky, W. T. H., Choi, H., et al. (2024) · Skin Research and Technology · doi:10.1111/srt.13730 · Cited by 23
Metadata
- Authors
- Kim, J.-P., Yang, Y.-S., Kim, J.-H., Lee, H.-H., Kim, E.-S., Moon, Y.-W., Kim, J.-Y., & Chung, J.-K.
- Affiliations
- Chonnam National University (KR)
- Corresponding author
- Chung, Jae-Keun
- Venue
- Korean Journal of Food Science and Technology
- Published
- 2012-08-31
- Volume
- 44
- Issue
- 4
- Pages
- 441-446
- ISSN
- 0367-6293 (ISSN-L)
- OpenAlex
- W2081749174
- Cited by
- 21
- References
- 16
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 1 · 2022: 3 · 2021: 2 · 2020: 3 · 2019: 1 · 2017: 1 · 2016: 2 · 2015: 2 · 2013: 3
- FWCI
- 1.11
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Korean Journal of Food Science and Technology (publishedVersion)
- Topics
- Health Sciences › Medicine › Biochemistry (medical) › Phytochemicals and Antioxidant Activities
- Life Sciences › Agricultural and Biological Sciences › Food Science › Food Quality and Safety Studies
- Life Sciences › Agricultural and Biological Sciences › Food Science › Food Nutrient Composition and Product Development
- Keywords
- Canavalia gladiata, sword bean, DPPH radical scavenging, antioxidant activity, crude protein, crude fat, amino acid composition, histidine, vitamin C, total polyphenols, total flavonoid content, mineral content, extraction yield, soybean, mungbean, legume extracts, methanol extraction, hexane extraction, ethyl acetate
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