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Cited by 7Enzyme Production and CharacterizationInfectious Diseases and MycologyAdvanced Scientific Research Methods

The DOI matches its Crossref record; 42 of the 42 references it cites were found in a registry or an index.

Screening and isolation of substitute-rennet producing thermophilic phycomycetes, by modified Warcup method and improved selective medium

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F, Khademi·2013·IOSR Journal of Agriculture and Veterinary Science
DOI 10.9790/2380-0223037
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Abstract

Fungal milk-clotting enzymes are as valuable as bovine Chymosin in the cheese industry.Members of the Mucoraceae family, have important biotechnological potential as some species are enzyme producers at industrial scale.In this study, 31 isolates of thermophilic Phycomycetes of different taxa were selected from different sample sites and were studied for their aspartic protease producing capabilities.For primary isolation, modified Warcup method using improved Phycomycetes selective solid media along with high temperature selective for thermophilic Mucorals were applied.The purpose of this study was to collect the local biotechnologically important strains of thermophilic phycomycetes capable of producing rennin-like proteases.Based on a high relative enzyme activity (REA), the majority (83.7%) of the 31 species of isolated thermophilic Phycomycetes were selected for next isolation steps.By trial production of acid proteases using shake flask fermentation, Milk clotting activity (MCA), and Protease activity (PA) of each fermentation broth of primary isolates were measured.Later, based on their high MCA/PA ratio, three isolates were chosen for macroscopic and microscopic examinations of each isolate determining their taxonomy identification and classification.The results showed that two of final isolates (HPA-01 and ZDO-16) belong to Rhizomucor family (R. nainitalensis and R. pusillus) and the third thermophilic Zygomycetes (ZDP-03) is a Rhizopus (R. rhizopodiformis).Of the 3 isolates, Rhizomucor nainitalensis was chosen for further studies and could be introduced as a commercially important isolate in future.

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Research works printed but not linked: 6

  1. Neelakantan S, Mohanty A, Kaushik JK. (1999): Production and use of microbial enzymes for dairy processing. Curr Sci, 77(1), 143-148.
  2. Alves MH, de Campos-Takaki GM, Okada K, Ferreira-Pessoa IH, Milanez AI. (2005): Detection of extracellular protease in Mucor species. Rev Iberoam Micol, 22(2), 114-117.
  3. Salar RK, Aneja K. (2007): Thermophilic fungi: taxonomy and biogeography. Journal of Agricultural Technology, 3(1), 77-107.
  4. Maheshwari R, Bharadwaj G, Bhat MK. (2000): Thermophilic fungi: their physiology and enzymes. Microbiol Mol Biol Rev, 64(3), 461-488.
  5. Ritter R. (1955): Physiological studies on Zygomycetes. Arch Mikrobiol, 22(3), 248-284.
  6. Shkurenko VO. (1967): Occurrence of thermophilic fungi of the order Mucorales in soil. Mikrobiol Zh, 29(1), 10-12.

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Metadata

Authors
F, K.
Venue
IOSR Journal of Agriculture and Veterinary Science
Publisher
IOSR Journals
Published
2013
Volume
2
Issue
2
Pages
30-37
ISSN
  • 2319-2372 (ISSN-L)
  • 2319-2380
DOI
10.9790/2380-0223037
OpenAlex
W2119226824
Cited by
7
References
42
Citations by year
2025: 2 · 2020: 1 · 2015: 2 · 2013: 2
FWCI
1.17
Type
journal article
Language
en
Open access
Locations
IOSR Journal of Agriculture and Veterinary Science (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Enzyme Production and Characterization
  • Health Sciences › Medicine › Infectious Diseases › Infectious Diseases and Mycology
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Advanced Scientific Research Methods
Keywords
Mucoraceae, aspartic protease, milk-clotting activity, Rhizomucor pusillus, selective culture media, shake flask fermentation, cheese industry

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