Kleinstiver, Benjamin P.

High-fidelity CRISPR-Cas9 nucleases with no detectable genome-wide off-target effects

Kleinstiver, B. P., Pattanayak, V., Prew, M. S., Tsai, S. Q., Nguyen, N. T., Zheng, Z., & Joung, J. K. (2016) · Nature

CRISPR and Genetic EngineeringRNA regulation and disease

Cited by 2,673

Engineered CRISPR-Cas9 nucleases with altered PAM specificities

Kleinstiver, B. P., Prew, M. S., Tsai, S. Q., Topkar, V. V., Nguyen, N. T., Zheng, Z., Gonzales, A. P. W., Li, Z., Peterson, R. T., Yeh, J.-R. J., et al. (2015) · Nature

CRISPR and Genetic EngineeringRNA regulation and disease

Cited by 1,624

Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants

Walton, R. T., Christie, K. A., Whittaker, M. N., & Kleinstiver, B. P. (2020) · Science

CRISPR and Genetic EngineeringRNA regulation and disease

Cited by 1,233

Enhanced proofreading governs CRISPR-Cas9 targeting accuracy

Chen, J. S., Dagdas, Y. S., Kleinstiver, B. P., Welch, M. M., Sousa, A. A., Harrington, L. B., Sternberg, S. H., Joung, J. K., Yildiz, A., & Doudna, J. A. (2017) · Nature

CRISPR and Genetic EngineeringRNA regulation and disease

Cited by 1,168

Genome-wide specificities of CRISPR-Cas Cpf1 nucleases in human cells

Kleinstiver, B. P., Tsai, S. Q., Prew, M. S., Nguyen, N. T., Welch, M. M., Lopez, J. M., McCaw, Z. R., Aryee, M. J., & Joung, J. K. (2016) · Nature Biotechnology

CRISPR and Genetic EngineeringRNA regulation and disease

Cited by 670

Broadening the targeting range of Staphylococcus aureus CRISPR-Cas9 by modifying PAM recognition

Kleinstiver, B. P., Prew, M. S., Tsai, S. Q., Nguyen, N. T., Topkar, V. V., Zheng, Z., & Joung, J. K. (2015) · Nature Biotechnology

CRISPR and Genetic EngineeringRNA regulation and disease

Cited by 611

High levels of AAV vector integration into CRISPR-induced DNA breaks

Hanlon, K. S., Kleinstiver, B. P., Garcia, S. P., Zaborowski, M. P., Volak, A., Spirig, S. E., Muller, A., Sousa, A. A., Tsai, S. Q., Bengtsson, N. E., et al. (2019) · Nature Communications

Virus-based gene therapy researchCRISPR and Genetic Engineering

Cited by 431

Prediction of off-target activities for the end-to-end design of CRISPR guide RNAs

Listgarten, J., Weinstein, M., Kleinstiver, B. P., Sousa, A. A., Joung, J. K., Crawford, J., Gao, K., Hoang, L., Elibol, M., Doench, J. G., et al. (2018) · Nature Biomedical Engineering

CRISPR and Genetic EngineeringRNA regulation and disease

Cited by 336

Astrocytic interleukin-3 programs microglia and limits Alzheimer’s disease

McAlpine, C. S., Park, J., Griciuc, A., Kim, E., Choi, S. H., Iwamoto, Y., Kiss, M. G., Christie, K. A., Vinegoni, C., Poller, W. C., et al. (2021) · Nature

Neuroinflammation and Neurodegeneration MechanismsAlzheimer's disease research and treatments

Cited by 322

PAM-flexible genome editing with an engineered chimeric Cas9

Zhao, L., Koseki, S. R. T., Silverstein, R. A., Amrani, N., Peng, C., Kramme, C., Savic, N., Pacesa, M., Rodríguez, T. C., Stan, T., et al. (2023) · Nature Communications

CRISPR and Genetic EngineeringRNA regulation and disease

Cited by 59