Hegde, Mudra

Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9

Doench, J. G., Fusi, N., Sullender, M., Hegde, M., Vaimberg, E. W., Donovan, K. F., Smith, I., Tothova, Z., Wilen, C., Orchard, R., et al. (2016) · Nature Biotechnology

CRISPR and Genetic EngineeringRNA Interference and Gene Delivery

Cited by 4,637

Rational design of highly active sgRNAs for CRISPR-Cas9-mediated gene inactivation

Doench, J. G., Hartenian, E., Graham, D. B., Tothova, Z., Hegde, M., Smith, I., Sullender, M., Ebert, B. L., Xavier, R. J., & Root, D. E. (2014) · Nature Biotechnology

CRISPR and Genetic EngineeringRNA regulation and disease

Cited by 1,617

Optimized libraries for CRISPR-Cas9 genetic screens with multiple modalities

Sanson, K. R., Hanna, R. E., Hegde, M., Donovan, K. F., Strand, C., Sullender, M. E., Vaimberg, E. W., Goodale, A., Root, D. E., Piccioni, F., et al. (2018) · Nature Communications

CRISPR and Genetic EngineeringChromatin Remodeling and Cancer

Cited by 1,043

Massively parallel assessment of human variants with base editor screens

Hanna, R. E., Hegde, M., Fagre, C. R., DeWeirdt, P. C., Sangree, A. K., Szegletes, Z., Griffith, A., Feeley, M. N., Sanson, K. R., Baidi, Y., et al. (2021) · Cell

CRISPR and Genetic EngineeringRNA regulation and disease

Cited by 316

Massively parallel assessment of human variants with base editor screens

Hanna, R. E., Hegde, M., Fagre, C. R., DeWeirdt, P. C., Sangree, A. K., Szegletes, Z., Griffith, A., Feeley, M. N., Sanson, K. R., Baidi, Y., et al. (2020) · Cell

CRISPR and Genetic EngineeringRNA regulation and disease

Cited by 314

Orthologous CRISPR–Cas9 enzymes for combinatorial genetic screens

Najm, F. J., Strand, C., Donovan, K. F., Hegde, M., Sanson, K. R., Vaimberg, E. W., Sullender, M. E., Hartenian, E., Kalani, Z., Fusi, N., et al. (2018) · Nature Biotechnology

CRISPR and Genetic EngineeringRNA Interference and Gene Delivery

Cited by 279

Uncoupling of sgRNAs from their associated barcodes during PCR amplification of combinatorial CRISPR screens

Hegde, M., Strand, C., Hanna, R. E., & Doench, J. G. (2018) · PLOS ONE

CRISPR and Genetic EngineeringRNA Interference and Gene Delivery

Cited by 66