Nakano, Hideo

N-terminal SKIK peptide tag markedly improves expression of difficult-to-express proteins in Escherichia coli and Saccharomyces cerevisiae

Ojima-Kato, T., Nagai, S., & Nakano, H. (2017) · Journal of Bioscience and Bioengineering

Protein purification and stabilityFungal and yeast genetics research

Cited by 65

Ecobody technology: Rapid monoclonal antibody screening method from single B cells using cell-free protein synthesis for antigen-binding fragment formation

Ojima-Kato, T., Nagai, S., & Nakano, H. (2017) · Scientific Reports

Monoclonal and Polyclonal Antibodies ResearchProtein purification and stability

Cited by 44

‘Zipbody’ leucine zipper-fused Fab in E. coli in vitro and in vivo expression systems

Ojima-Kato, T., Fukui, K., Yamamoto, H., Hashimura, D., Miyake, S., Hirakawa, Y., Yamasaki, T., Kojima, T., & Nakano, H. (2016) · Protein Engineering Design and Selection

Monoclonal and Polyclonal Antibodies ResearchProtein purification and stability

Cited by 35

In vitro generation of rabbit anti-Listeria monocytogenes monoclonal antibody using single cell based RT-PCR linked cell-free expression systems

Ojima-Kato, T., Hashimura, D., Kojima, T., Minabe, S., & Nakano, H. (2015) · Journal of Immunological Methods

Monoclonal and Polyclonal Antibodies ResearchMolecular Biology Techniques and Applications

Cited by 31

A novel strategy for generation of monoclonal antibodies from single B cells using rt-PCR technique and in vitro expression

Jiang, X. P., Suzuki, H., Hanai, Y., Wada, F., Hitomi, K., Yamane, T., & Nakano, H. (2006) · Biotechnology Progress

Monoclonal and Polyclonal Antibodies ResearchProtein purification and stability

Cited by 25

Rapid Generation of Monoclonal Antibodies from Single B Cells by Ecobody Technology

Ojima-Kato, T., Morishita, S., Uchida, Y., Nagai, S., Kojima, T., & Nakano, H. (2018) · Antibodies

Monoclonal and Polyclonal Antibodies ResearchProtein purification and stability

Cited by 23

SKIK-zipbody-alkaline phosphatase, a novel antibody fusion protein expressed in Escherichia coli cytoplasm

Ritthisan, P., Ojima-Kato, T., Damnjanović, J., Kojima, T., & Nakano, H. (2018) · Journal of Bioscience and Bioengineering

Monoclonal and Polyclonal Antibodies ResearchProtein purification and stability

Cited by 13

In vitro generation of anti-hepatitis B monoclonal antibodies from a single plasma cell using single-cell RT-PCR and cell-free protein synthesis

Sabrina, Y., Ali, M., & Nakano, H. (2010) · Journal of Bioscience and Bioengineering

Monoclonal and Polyclonal Antibodies ResearchProtein purification and stability

Cited by 10

Development of a dual monoclonal antibody sandwich enzyme-linked immunosorbent assay for the detection of swine influenza virus using rabbit monoclonal antibody by Ecobody technology

Sila-on, D., Chertchinnapa, P., Shinkai, Y., Kojima, T., & Nakano, H. (2020) · Journal of Bioscience and Bioengineering

Influenza Virus Research StudiesMonoclonal and Polyclonal Antibodies Research

Cited by 7