The DOI matches its Crossref record.
Techniques for Mammalian Cell Tissue Culture
Abstract
This appendix opens with detailed discussions on the latest principles of sterile technique and preparation of culture media. Step‐by‐step protocols describe trypsinizing and subculturing monolayer cultures, passaging suspension cultures, freezing and thawing cells, counting cells using a hemacytometer, and preparing cells for transport.
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“In order to detect the mycoplasma contamination after harvesting, each cell line was cultured in an antibiotics-free medium for a week (at least 4-5 days) without exchanging the medium (Phelan 2006(Phelan , 2007)).”
From Real-time PCR assay is superior to other methods for the detection of mycoplasma contamination in the cell lines of the National Cell Bank of Iran (2016) · cites this paper as Phelan 2006
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Research works printed but not linked: 10
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Molla Kazemiha, V., Bonakdar, S., Amanzadeh, A., Azari, S., Memarnejadian, A., Shahbazi, S., Shokrgozar, M. A., & Mahdian, R. (2016) · Cytotechnology · doi:10.1007/s10616-015-9862-0 · Cited by 20
Koidl, S., & Timmers, H. T. M. (2021) · Current Protocols · doi:10.1002/cpz1.266 · Cited by 21
“…or equivalent Additional reagents and equipment for cell culture and cell counting using a hemocytometer and trypan blue (Current Protocols article: Phelan, 2006) NOTE: Make sure all buffers and materials are at room temperature, except the 100 mM CaCl2 solution and digitonin buffer, which should…”
MethodUsing Human Primary Foreskin Fibroblasts to Study Cellular Damage and Mitochondrial Dysfunction
Nadalutti, C. A., & Wilson, S. H. (2020) · Current Protocols in Toxicology · doi:10.1002/cptx.99 · Cited by 18
“Count cells using a hemocytometer and trypan blue exclusion (see Current Protocols article: Phelan, 2007).”
MethodInfluentialMontel-Hagen, A., Tsai, S. C., Seet, C., & Crooks, G. (2022) · Current Protocols · doi:10.1002/cpz1.403 · Cited by 13
Preparation of Mammalian Nascent RNA for Long Read Sequencing
Reimer, K. A., & Neugebauer, K. M. (2020) · Current Protocols in Molecular Biology · doi:10.1002/cpmb.128 · Cited by 12
“Count actively growing MEL cells using a hemocytometer and trypan blue (see Cur- rent Protocols article: Phelan, 2006).”
MethodGel‐Based and Gel‐Free Phosphoproteomics to Measure and Characterize Mitochondrial Phosphoproteins
Thongboonkerd, V., & Chaiyarit, S. (2022) · Current Protocols · doi:10.1002/cpz1.390 · Cited by 10
“Count viable cells with trypan blue solution, a hemacytometer, and a microscope (see Current Protocols article; Phelan, 2007) and seed 2 × 106 cells in a new 75- cm2 culture flask containing 15 ml fresh complete MEM.”
MethodXeno‐Free Reprogramming of Peripheral Blood Mononuclear Erythroblasts on Laminin‐521
Skorik, C., Mullin, N. K., Shi, M. A., Zhang, Y., Hunter, P., Tang, Y., Hilton, B. M., & Schlaeger, T. M. (2020) · Current Protocols in Stem Cell Biology · doi:10.1002/cpsc.103 · Cited by 8
Assessment of DNA Susceptibility to Denaturation as a Marker of Chromatin Structure
Darżynkiewicz, Z., Halicka, D. H., Zhao, H. L., & Li, J. (2019) · Current Protocols in Cytometry · doi:10.1002/cpcy.65 · Cited by 1
“For adherent cells: Collect cells from flasks or Petri dishes by trypsinization (Phelan, 2006), and pool the trypsinized (detached) cells with cells floating in the medium (which will consist mostly of detached mitotic and dead cells) by centrifuging 5 min at 300 × g, room temperature.”
BackgroundMethod
Metadata
- Authors
- Phelan, M. C.
- Affiliations
- Molecular Pathology Laboratory Network (United States) (US)
- Corresponding author
- Phelan, Mary C.
- Venue
- Current Protocols in Molecular Biology
- Publisher
- Wiley
- Published
- 2006-04
- Volume
- 74
- Issue
- 1
- Pages
- A.3C.1-A.3C.18
- ISSN
- 1934-3639 (ISSN-L)
- 1934-3647
- OpenAlex
- W2167554133
- Cited by
- 89
- References
- 0
- Citations by year
- 2023: 1 · 2022: 1 · 2021: 6 · 2020: 6 · 2019: 1 · 2017: 6 · 2016: 8 · 2015: 7 · 2014: 2 · 2013: 4 · 2012: 5
- FWCI
- 3.94
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Current Protocols in Molecular Biology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Viral Infectious Diseases and Gene Expression in Insects
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant tissue culture and regeneration
- Keywords
- mammalian cell culture, surgical asepsis, culture media preparation, trypsinization, subculturing, monolayer culture, cell freezing and thawing, cell counting, hemacytometer, cell transport
- MeSH terms
- Culture Media, Freezing, Indicators and Reagents, Infertility, Trypsin, Cell Culture Techniques
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