Preparation of Basement Membrane Components from EHS Tumors
Abstract
This unit describes methods for passaging and harvesting the basement membrane matrix‐producing EHS tumor and for the subsequent isolation of a crude mixture of basement membrane components termed Matrigel, which promotes the differentiation of a variety of epithelial, endothelial, and neuronal cells. Procedures for the isolation of the adhesive glycoprotein laminin‐1 and of type IV collagen are also included. Support protocols cover the maintenance and harvesting of EHS tumors in mice and maintenance of mice on a lathrogenic diet.
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Production of Matrigel requires approximately 16 mice per 100 mL.
“with approximately 16 mice required per 100 mL Matrigel (Kibbey, 1994; Kleinman, 2001; Berg and Kurreck, 2021).”
From Challenges and solutions in transitioning to animal-free standards: a comprehensive analysis of components in human cell-based developmental neurotoxicity assays (2026) · cites this paper as Kleinman, 2001
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Willemse, J., van Tienderen, G., van Hengel, E., Schurink, I., van der Ven, D., Kan, Y., de Ruiter, P., Rosmark, O., Westergren-Thorsson G, G., Schneeberger, K., et al. (2022) · Biomaterials · doi:10.1016/j.biomaterials.2022.121473 · Cited by 106 · see citing works
A heparin mimetic isolated from a marine shrimp suppresses neovascularization
DREYFUSS, J. L., REGATIERI, C. V., LIMA, M. A., PAREDES‐GAMERO, E. J., BRITO, A. S., CHAVANTE, S. F., BELFORT JR, R., FARAH, M. E., & NADER, H. B. (2010) · Journal of Thrombosis and Haemostasis · doi:10.1111/j.1538-7836.2010.03916.x · Cited by 38 · see citing works
Spänle, J. V., Haiber, L. M., & Seeger, B. (2026) · Frontiers in Toxicology · doi:10.3389/ftox.2026.1800157 · Cited by 1 · see citing works
- Authors
- Kleinman, H. K.
- Affiliations
- National Institute of Dental Research/NIH Bethesda Maryland
- Venue
- Current Protocols in Cell Biology
- Publisher
- Wiley
- Published
- 1998-10
- Volume
- 00
- Issue
- 1
- ISSN
- 1934-2500
- 1934-2616
- Cited by
- 24
- References
- 0
- Language
- en
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