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Isothermal Titration Calorimetry
Abstract
In the last two decades, isothermal titration calorimetry (ITC) has become the preferred technique to determine the binding energetics of biological processes, including protein‐ligand binding, protein‐protein binding, DNA‐protein binding, protein‐carbohydrate binding, protein‐lipid binding, and antigen‐antibody binding. In this unit several protocols are presented, ranging from the basic ones that are aimed at characterizing binding of moderate affinity to advanced protocols that are aimed at determining very high or very low affinity binding processes. Also, alternate protocols for special cases (homodimeric proteins and unstable proteins) and additional information accessible by ITC (heat capacity and protonation/deprotonation processes coupled to binding) are presented.
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Citations
Cited by
Entropy-Enthalpy Compensation: Role and Ramifications in Biomolecular Ligand Recognition and Design
Chodera, J. D., & Mobley, D. L. (2013) · Annual Review of Biophysics · doi:10.1146/annurev-biophys-083012-130318 · Cited by 531
Lafitte, D., Lamour, V., Tsvetkov, P. O., Makarov, A. A., Klich, M., Deprez, P., Moras, D., Briand, C., & Gilli, R. (2002) · Biochemistry · doi:10.1021/bi0159837 · Cited by 334
Solís, D., Bovin, N. V., Davis, A. P., Jiménez-Barbero, J., Romero, A., Roy, R., Smetana, K., & Gabius, H.-J. (2015) · Biochimica et Biophysica Acta (BBA) - General Subjects · doi:10.1016/j.bbagen.2014.03.016 · Cited by 222
A multi-step, dynamic allosteric model of testosterone's binding to sex hormone binding globulin
Zakharov, M. N., Bhasin, S., Travison, T. G., Xue, R., Ulloor, J., Vasan, R. S., Carter, E., Wu, F., & Jasuja, R. (2015) · Molecular and Cellular Endocrinology · doi:10.1016/j.mce.2014.09.001 · Cited by 81
The thermodynamics of Pr55Gag-RNA interaction regulate the assembly of HIV
Tanwar, H. S., Khoo, K. K., Garvey, M., Waddington, L., Leis, A., Hijnen, M., Velkov, T., Dumsday, G. J., McKinstry, W. J., & Mak, J. (2017) · PLOS Pathogens · doi:10.1371/journal.ppat.1006221 · Cited by 51
Metadata
- Authors
- Velázquez‐Campoy, A., Ohtaka, H., Nezami, A., Muzammil, S., & Freire, E.
- Venue
- Current Protocols in Cell Biology
- Publisher
- Wiley
- Published
- 2004-06
- Volume
- 23
- Issue
- 1
- Pages
- Unit 17.8-Unit 17.8
- ISSN
- 1934-2500 (ISSN-L)
- 1934-2616
- OpenAlex
- W1979447111
- Cited by
- 236
- References
- 0
- FWCI
- 1.11
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: Isothermal Titration Calorimetry. https://app.cochat.ai/papers/10.1002/0471143030.cb1708s23
- The paper
- Velázquez‐Campoy, A., Ohtaka, H., Nezami, A., Muzammil, S., & Freire, E. (2004). Isothermal Titration Calorimetry. Current Protocols in Cell Biology. https://doi.org/10.1002/0471143030.cb1708s23
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
