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Current Protocols in Microbiology
Open accessAbstract
In less than a year, SARS-CoV-2 managed to displace Mycobacterium tuberculosis (Mtb) as the leading cause of death worldwide due to a single infectious agent.Both pathogens affect the respiratory tract, mainly the lungs.However, the impact that a possible Mtb + SARS-CoV-2 co-infection can have on the host response is still unknown.Herein we depict a rigorous system to evaluate the complex interaction between two simultaneous infections in vitro in a lung epithelial cell line (A549).Overall, the process includes eight steps: (1) Mtb culture, (2) cell maintenance, (3) preparation of viral stocks, (4) determination of infectious titers, (5) Mtb and SARS-CoV-2 co-infection, (6) determination of intracellular bacterial load, (7) SARS-CoV-2 viability test, and (8) optional UV-C validation assay for infectivity reduction.This comprehensive protocol will allow experimentalists to study the pathogenesis of co-infection in vitro and facilitate collaborative work in the literature.
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Cited by
Rubinstein, M. R., Wang, X., Liu, W., Hao, Y., Cai, G., & Han, Y. W. (2013) · Cell Host & Microbe · doi:10.1016/j.chom.2013.07.012 · Cited by 2,476
Droplet microfluidics for high-throughput biological assays
Guo, M. T., Rotem, A., Heyman, J. A., & Weitz, D. A. (2012) · Lab on a Chip · doi:10.1039/c2lc21147e · Cited by 1,040
Drug Tolerance in Replicating Mycobacteria Mediated by a Macrophage-Induced Efflux Mechanism
Adams, K. N., Takaki, K., Connolly, L. E., Wiedenhoft, H., Winglee, K., Humbert, O., Edelstein, P. H., Cosma, C. L., & Ramakrishnan, L. (2011) · Cell · doi:10.1016/j.cell.2011.02.022 · Cited by 530
miRNAs control gene expression in the single-cell alga Chlamydomonas reinhardtii
Molnár, A., Schwach, F., Studholme, D. J., Thuenemann, E. C., & Baulcombe, D. C. (2007) · Nature · doi:10.1038/nature05903 · Cited by 501
Pamp, S. J., Gjermansen, M., Johansen, H. K., & Tolker‐Nielsen, T. (2008) · Molecular Microbiology · doi:10.1111/j.1365-2958.2008.06152.x · Cited by 486
Metadata
- Venue
- John Wiley & Sons, Inc. eBooks
- Publisher
- John Wiley & Sons, Inc.
- Published
- 2005-07-15
- OpenAlex
- W2520102457
- Cited by
- 405
- References
- 0
- Type
- reference entry
- Language
- en
- Open access
- gold
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