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Cited by 54Nanoplatforms for cancer theranosticsNanoparticle-Based Drug DeliveryGlioma Diagnosis and Treatment

Cell Membrane‐Coated Magnetic Nanocubes with a Homotypic Targeting Ability Increase Intracellular Temperature due to ROS Scavenging and Act as a Versatile Theranostic System for Glioblastoma Multiforme

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Tapeinos, Christos, Tomatis, Francesca, Battaglini, Matteo, Larrañaga, Aitor, Marino, Attilio, Telleria, Iker Aguirrezabal, Angelakeris, Makis, Debellis, Doriana +7 more·2019·Advanced Healthcare Materials
DOI 10.1002/adhm.201900612PMC PMC6754263
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Abstract

In this study, hybrid nanocubes composed of magnetite (Fe 3 O 4) and manganese dioxide (MnO 2), coated with U‐251 MG cell‐derived membranes (CM‐NCubes) are synthesized. The CM‐NCubes demonstrate a concentration‐dependent oxygen generation (up to 15%), and, for the first time in the literature, an intracellular increase of temperature (6 °C) due to the exothermic scavenging reaction of hydrogen peroxide (H 2 O 2) is showed. Internalization studies demonstrate that the CM‐NCubes are internalized much faster and at a higher extent by the homotypic U‐251 MG cell line compared to other cerebral cell lines. The ability of the CM‐NCubes to cross an in vitro model of the blood‐brain barrier is also assessed. The CM‐NCubes show the ability to respond to a static magnet and to accumulate in cells even under flowing conditions. Moreover, it is demonstrated that 500 µg mL−1 of sorafenib‐loaded or unloaded CM‐NCubes are able to induce cell death by apoptosis in U‐251 MG spheroids that are used as a tumor model, after their exposure to an alternating magnetic field (AMF). Finally, it is shown that the combination of sorafenib and AMF induces a higher enzymatic activity of caspase 3 and caspase 9, probably due to an increment in reactive oxygen species by means of hyperthermia.

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  • Brain tumor cell membrane tends to have homotypic targeting, long-time circulation, and BBB crossing abilities.

    “Brain tumor cell membrane, originated from brain tumor cells, tend to have homotypic targeting, long-time circulation, and BBB crossing abilities.”

    From The blood–brain barrier: Structure, regulation and drug delivery (2023) · cites this paper as [199]

    The Introduction (page unavailable) says cell-membrane coatings can inhibit macrophage uptake and often enable homotypic targeting. The Blood-brain barrier crossing section (page unavailable) reports passage by membrane-coated nanocubes in an in vitro BBB model. The paper does not establish long circulation times or BBB-crossing ability for brain-tumor cell membranes generally. (LLM, conf=0.87)

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Authors
Tapeinos, C., Tomatis, F., Battaglini, M., Larrañaga, A., Marino, A., Telleria, I. A., Angelakeris, M., Debellis, D., Drago, F., Brero, F., Arosio, P., Lascialfari, A., Petretto, A., Sinibaldi, E., & Ciofani, G.
Affiliations
  • Italian Institute of Technology (IT)
  • Politecnico di Torino (IT)
  • Scuola Superiore Sant'Anna (IT)
  • University of the Basque Country (ES)
  • Department of Chemical and Environmental Engineering Engineering School of the University of the Basque Country (UPV/EHU) 48013 Bilbao Spain
  • Aristotle University of Thessaloniki (GR)
  • University of Pavia (IT)
  • University of Milan (IT)
  • Istituto Giannina Gaslini (IT)
Corresponding author
  • Tapeinos, Christos
  • Ciofani, Gianni
ORCID
Venue
Advanced Healthcare Materials
Publisher
Wiley
Published
2019-08-07
Volume
8
Issue
18
Pages
e1900612-e1900612
ISSN
  • 2192-2640 (ISSN-L)
  • 2192-2659
DOI
10.1002/adhm.201900612
PMC
PMC6754263
OpenAlex
W2966870853
Cited by
54
References
4
Citations by year
2026: 5 · 2025: 5 · 2024: 6 · 2023: 9 · 2022: 8 · 2021: 10 · 2020: 8 · 2019: 2 · 2018: 1
FWCI
2.47
Type
journal article
Language
en
Open access
Licence
Locations
  • Advanced Healthcare Materials (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • Research Explorer (The University of Manchester) (publishedVersion, other-oa)
  • Europe PMC (PubMed Central) (acceptedVersion)
  • PubMed Central (acceptedVersion)
  • PORTO@Iris (submittedVersion, other-oa)
  • Communities in ADDI (University of the Basque Country) (submittedVersion, cc-by)
  • Archivio Istituzionale della Ricerca (Universita Degli Studi Di Milano) (submittedVersion, other-oa)
  • Hispana (submittedVersion, cc-by)
  • CINECA IRIS Institutional Research Information System (Sant'Anna School of Advanced Studies) (submittedVersion)
Funders
European Research Council (709613)
Topics
  • Physical Sciences › Engineering › Biomedical Engineering › Nanoplatforms for cancer theranostics
  • Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
  • Health Sciences › Medicine › Genetics › Glioma Diagnosis and Treatment
Keywords
magnetite, manganese dioxide, cell membrane coating, glioblastoma, hyperthermia, reactive oxygen species scavenging, hydrogen peroxide, alternating magnetic field, theranostics, blood-brain barrier penetration, U251 cells, apoptosis, caspase-3, caspase-9, cellular internalization
MeSH terms
Protein Corona, Dynamic Light Scattering, Theranostic Nanomedicine, Blood-Brain Barrier, Cell Membrane, Endocytosis, Fluorescence, Glioblastoma, Humans, Hyperthermia, Induced, Oxygen, Temperature, Apoptosis, Reactive Oxygen Species, Cell Line, Tumor, Magnetite Nanoparticles, Drug Liberation

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