Preparation of Decellularized Triphasic Hierarchical Bone‐Fibrocartilage‐Tendon Composite Extracellular Matrix for Enthesis Regeneration
Open accessAbstract
Tendon to bone (enthesis) rupture, which may cause disability and persistent pain, shows high rate of re‐rupture after surgical repair. Tendon or enthesis scaffolds have been widely studied, but few of these materials can recapitulate the tissue continuity. Thus, this study is conducted to prepare a triphasic decellularized bone‐fibrocartilage‐tendon (D‐BFT) composite scaffold. The D‐BFT scaffold is developed using a combination of physical, chemical, and enzymatic treatments using liquid nitrogen, Triton‐X 100, sodium‐dodecyl sulfate, and DNase I, which effectively removes the cell components while preserving the biological composite and microstructure. Moreover, the mechanical properties of D‐BFT are highly preserved and similar to those of the human Achilles tendon. Additionally, in vitro, mesenchymal stem cells (MSCs) adhered, proliferated, and infiltrated into the D‐BFT scaffold, and MSC differentiation is confirmed by up‐regulation of osteogenic‐related and tenogenic‐related genes. The repair outcomes are explored by applying the D‐BFT scaffold in the model of femur‐tibia defects in vivo, which shows good repair results. Thus, the D‐BFT scaffold developed in this study is a promising graft for enthesis regeneration.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
DNase is typically applied after chemical detergents to solubilize cell membranes and dissociate their inner structure, enhancing DNase infiltration and efficiency within tissues.
“Deoxyribonuclease (DNase), a common nuclease, is typically applied after chemical detergents to solubilize cell membranes and dissociate their inner structure, enhancing DNase infiltration and efficiency within tissues [[152], [153], [154]].”
From Liver tissue engineering using decellularized scaffolds: Current progress, challenges, and opportunities (2024) · cites this paper as [154]
Cites
Decellularization of tissues and organs
Gilbert, T. W., Sellaro, T. L., & Badylak, S. F. (2006) · Biomaterials · doi:10.1016/j.biomaterials.2006.02.014 · Cited by 2,222 · see citing works
Yang, B., Zhang, Y., Zhou, L., Sun, Z., Zheng, J., Chen, Y., & Dai, Y. (2010) · Tissue Engineering Part C: Methods · doi:10.1089/ten.tec.2009.0311 · Cited by 206 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Hussein, K. H., Ahmadzada, B., Correa, J. C., Sultan, A., Wilken, S., Amiot, B., & Nyberg, S. L. (2024) · Bioactive Materials · doi:10.1016/j.bioactmat.2024.06.001 · Cited by 28 · see citing works
“Deoxyribonuclease (DNase), a common nuclease, is typically applied after chemical detergents to sol-ubilize cell membranes and dissociate their inner structure, enhancing DNase infiltration and efficiency within tissues [152 – 154].”
BackgroundShi, Q., Chen, Y., Li, M., Zhang, T., Ding, S., Xu, Y., Hu, J., Chen, C., & Lu, H. (2020) · Annals of Translational Medicine · doi:10.21037/atm-20-3661 · Cited by 16 · see citing works
- Authors
- Su, M., Zhang, Q., Zhu, Y., Wang, S., Lv, J., Sun, J., Qiu, P., Fan, S., Jin, K., Chen, L., & Lin, X.
- Affiliations
- First Affiliated Hospital of Wenzhou Medical University (CN)
- Sir Run Run Shaw Hospital (CN)
- Wenzhou Medical University (CN)
- Corresponding author
- Jin, Keke
- Chen, Lei
- Lin, Xianfeng
- Venue
- Advanced Healthcare Materials
- Publisher
- Wiley
- Published
- 2019-08-28
- Volume
- 8
- Issue
- 19
- Pages
- e1900831-e1900831
- ISSN
- 2192-2640 (ISSN-L)
- 2192-2659
- OpenAlex
- W2970225951
- Cited by
- 50
- References
- 2
- Citations by year
- 2025: 6 · 2024: 8 · 2023: 10 · 2022: 11 · 2021: 10 · 2020: 5
- FWCI
- 6.21
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Advanced Healthcare Materials (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Basic Research Program of China (2018YFC1105202)
- Topics
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
- Health Sciences › Medicine › Orthopedics and Sports Medicine › Tendon Structure and Treatment
- Keywords
- enthesis regeneration, decellularized extracellular matrix, Achilles tendon, mesenchymal stem cells, osteogenic differentiation, tenogenic differentiation, scaffold mechanical properties
- MeSH terms
- Achilles Tendon, Animals, Bone and Bones, Cell Adhesion, Cell Differentiation, Collagen, Extracellular Matrix, Humans, Mice, Inbred C57BL, Nitrogen, Osteogenesis, Regeneration, Stress, Mechanical, Tissue Engineering, Regenerative Medicine, Cell Proliferation, Mice, Fibrocartilage, Tissue Scaffolds, X-Ray Microtomography, Mesenchymal Stem Cells
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
