The DOI matches its Crossref record; 235 of the 235 references it cites were found in a registry or an index.
Bioengineering Approaches for the Advanced Organoid Research
Open accessAbstract
Recent advances in 3D cell culture technology have enabled scientists to generate stem cell derived organoids that recapitulate the structural and functional characteristics of native organs. Current organoid technologies have been striding toward identifying the essential factors for controlling the processes involved in organoid development, including physical cues and biochemical signaling. There is a growing demand for engineering dynamic niches characterized by conditions that resemble in vivo organogenesis to generate reproducible and reliable organoids for various applications. Innovative biomaterial‐based and advanced engineering‐based approaches have been incorporated into conventional organoid culture methods to facilitate the development of organoid research. The recent advances in organoid engineering, including extracellular matrices and genetic modulation, are comprehensively summarized to pinpoint the parameters critical for organ‐specific patterning. Moreover, perspective trends in developing tunable organoids in response to exogenous and endogenous cues are discussed for next‐generation developmental studies, disease modeling, and therapeutics.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The source backs part of the claim, not all of it — or backs it for a different population, condition or comparison.
In the citing paper
“However, organoid cultures exhibit significant heterogeneity, variable complexity in cellular composition, can undergo poorly-controlled morphogenesis in self-assembly process, and often lack stromal, vascular and immunological components4,13.”
From Organoids (2022) · cites this paper as [4]
Experimental — accuracy not yet measuredThe cited source says what the claim attributes to it.
In the citing paper
“However, organoid cultures exhibit significant heterogeneity, variable complexity in cellular composition, can undergo poorly-controlled morphogenesis in self-assembly process, and often lack stromal, vascular and immunological components4,13.”
From Organoids (2022) · cites this paper as [4]
The cited source says what the claim attributes to it.
In the citing paper
“However, organoid cultures exhibit significant heterogeneity, variable complexity in cellular composition, can undergo poorly-controlled morphogenesis in self-assembly process, and often lack stromal, vascular and immunological components4,13.”
From Organoids (2022) · cites this paper as [4]
The cited source says what the claim attributes to it.
In the citing paper
“However, organoid cultures exhibit significant heterogeneity, variable complexity in cellular composition, can undergo poorly-controlled morphogenesis in self-assembly process, and often lack stromal, vascular and immunological components4,13.”
From Organoids (2022) · cites this paper as [4]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Takahashi, K., & Yamanaka, S. (2006) · Cell · doi:10.1016/j.cell.2006.07.024 · Cited by 26,673
Genome engineering using the CRISPR-Cas9 system
Ran, F. A., Hsu, P. D., Wright, J., Agarwala, V., Scott, D. A., & Zhang, F. (2013) · Nature Protocols · doi:10.1038/nprot.2013.143 · Cited by 11,133
Alginate: Properties and biomedical applications
Lee, K. Y., & Mooney, D. J. (2011) · Progress in Polymer Science · doi:10.1016/j.progpolymsci.2011.06.003 · Cited by 8,477
Self-citationMolecular Mechanisms of Stress-Responsive Changes in Collagen and Elastin Networks in Skin
Aziz, J., Shezali, H., Radzi, Z., Yahya, N. A., Abu Kassim, N. H., Czernuszka, J., & Rahman, M. T. (2016) · Skin Pharmacology and Physiology · doi:10.1159/000447017 · Cited by 7,323
Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche
Sato, T., Vries, R. G., Snippert, H. J., van de Wetering, M., Barker, N., Stange, D. E., van Es, J. H., Abo, A., Kujala, P., Peters, P. J., et al. (2009) · Nature · doi:10.1038/nature07935 · Cited by 7,204
Cerebral organoids model human brain development and microcephaly
Lancaster, M. A., Renner, M., Martin, C.-A., Wenzel, D., Bicknell, L. S., Hurles, M. E., Homfray, T., Penninger, J. M., Jackson, A. P., & Knoblich, J. A. (2013) · Nature · doi:10.1038/nature12517 · Cited by 5,372
Rheinwatd, J. G., & Green, H. (1975) · Cell · doi:10.1016/s0092-8674(75)80001-8 · Cited by 4,581
Matrix Crosslinking Forces Tumor Progression by Enhancing Integrin Signaling
Levental, K. R., Yu, H., Kass, L., Lakins, J. N., Egeblad, M., Erler, J. T., Fong, S. F. T., Csiszar, K., Giaccia, A., Weninger, W., et al. (2009) · Cell · doi:10.1016/j.cell.2009.10.027 · Cited by 4,101
Visualization and analysis of gene expression in tissue sections by spatial transcriptomics
Ståhl, P. L., Salmén, F., Vickovic, S., Lundmark, A., Navarro, J. F., Magnusson, J., Giacomello, S., Asp, M., Westholm, J. O., Huss, M., et al. (2016) · Science · doi:10.1126/science.aaf2403 · Cited by 3,834
Sato, T., Stange, D. E., Ferrante, M., Vries, R. G. J., van Es, J. H., van den Brink, S., van Houdt, W. J., Pronk, A., van Gorp, J., Siersema, P. D., et al. (2011) · Gastroenterology · doi:10.1053/j.gastro.2011.07.050 · Cited by 3,753
Research works printed but not linked: 7
- Moscona A., 1952, J. Anat., 86, 287
- Qian X., 2019, Development, 146
- Borenstein J. T., 2004, Architecture and Application of Biomaterials and Biomolecular Materials, 9
- Maile S., 2000, Histol. Histopathol., 15, 403
- Luo X., 2020, Acta Biomater.
- Skardal A., 2016, J. Vis. Exp.
- Lee H. J., 2021, ACS Biomater. Sci. Eng.
Cited by
Sorted by citations
Items 201–218 of 218
Gupta, V., Hill, M. L., Nortz, S. P., Choudhary, Y., Rana, A., & Dick, J. E. (2026) · Angewandte Chemie · doi:10.1002/ange.8508105
Chen, J., Xu, J., Aikebaier, S., Liang, Y., Zhou, X., Zhu, X. M., & Zhou, X. (2026) · Advanced Healthcare Materials · doi:10.1002/adhm.71370
Cardiovascular Organoids With Adjustable Endothelial Composition via SOX17‐Engineered hPSCs
Liang, P.-Y., Jin, G., Petrucci, N. R., Lian, X. L., & Bao, X. (2026) · Biotechnology and Bioengineering · doi:10.1002/bit.70275
Chen, R., Yin, B., Zhang, Y., Peng, J., Guo, X., & Wu, W. (2026) · iScience · doi:10.1016/j.isci.2026.116487
Self-citationAksentijević, M., Mikerova, M. S., Reshetnikov, V., & Turnić, T. N. (2026) · Medicinski casopis · doi:10.5937/mckg60-63075
Li, J., Jin, R., Zhang, X., CHEN, Y., Ai, H., Mei, X., Sun, Y., Zheng, L., Wang, G., Bao, Y., et al. (2026) · MethodsX · doi:10.1016/j.mex.2026.104016
Dielectric levitation optical tweezers for powerful mesoscale biomanipulation
Liu, H., Fu, R., Nan, F., Guo, Z., Zhao, M., Zhang, Y., Guo, S., Li, H., Chen, K., Chu, B., et al. (2026) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2533103123
Self-citationIn Situ Construction of Fascial Analogues Induced by Low-Growth-Factor Matrigel
Yue, J., Guo, Y., Huang, H., Zhang, Z., Mei, L., & Chen, Q. (2026) · Animals · doi:10.3390/ani16152287
The migratory locust (Locusta migratoria): A large-sized genome model for modern biology
Cao, L., Chen, Q., Zhang, C., He, J., Ding, D., & Kang, L. (2026) · Model Organisms Research · doi:10.1016/j.mores.2026.100010
Organoids in endometrial diseases: from bench to bedside
Yang, D., Zhang, J., Lv, F., Ma, S., Cui, J., & Liu, D. (2026) · Biofabrication · doi:10.1088/1758-5090/ae93ff
Bian, S., Liu, S., Chi, H., Chen, L., Tan, S., Jiang, N., Vankelecom, H. E. J., Zhang, X., & Li, C. (2026) · Soft Science · doi:10.20517/ss.2026.117
Yuan, Y., Luo, X., Tang, L., He, T., Zou, X., Feng, L., Su, B., Wu, J., Li, J., & Xiao, D. (2026) · Cell and Tissue Banking · doi:10.1007/s10561-026-10226-2
Biomaterials in organoid research: current state and future directions
Klasen, L., Nasehi, R., Selzener, L., & Laporte, L. D. (2026) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2026.1895558
Mendoza, R., Cruz, I., & Lopez, C. (2026) · Interdisciplinary Research in Medical Sciences Specialty · doi:10.51847/yxm9sqygaq
DNA hydrogels in bone tissue engineering: where molecular ingenuity meets skeletal renaissance
Wang, J., Li, M., Zhao, P., & Li, J. (2026) · Journal of Materials Chemistry B · doi:10.1039/d6tb01765g · Cited by 1
Organoids: Current Applications and Future Directions
Leng, Y., Wang, Y., Cao, C., Deng, F., Lian, W., Chen, X., Lin, M., Zeng, Z., Mo, D., Li, S., et al. (2026) · MedComm · doi:10.1002/mco2.70965
Wu, Y., Hao, S., Gu, Y., Li, H., Chen, Z., Yu, Y., Yao, K., & 傅秋黎 (2026) · Stem Cell Reports · doi:10.1016/j.stemcr.2026.103122
Tang, X., Xu, H., Liu, X., Yang, Y., & 李章华 (2026) · Advanced Healthcare Materials · doi:10.1002/adhm.71911
Metadata
- Authors
- Yi, S. A., Zhang, Y., Rathnam, C., Pongkulapa, T., & Lee, K.
- Affiliations
- Rutgers, The State University of New Jersey (US)
- Corresponding author
- Lee, Ki‐Bum
- Venue
- Advanced Materials
- Publisher
- Wiley
- Published
- 2021-09-24
- Volume
- 33
- Issue
- 45
- Pages
- e2007949-e2007949
- ISSN
- 0935-9648 (ISSN-L)
- 1521-4095
- PMC
- PMC8682947
- OpenAlex
- W3199075493
- Cited by
- 218
- References
- 235
- Citations by year
- 2026: 50 · 2025: 76 · 2024: 53 · 2023: 26 · 2022: 12 · 2021: 1
- FWCI
- 1.95
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Advanced Materials (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institute on Deafness and Other Communication Disorders (R01DC016612)
- National Institute of Arthritis and Musculoskeletal and Skin Diseases (R21AR071101)
- National Institute of Biomedical Imaging and Bioengineering (T32EB005583)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
- Keywords
- organoids, organoid engineering, 3D cell culture, stem cell-derived organoids, extracellular matrix, gene modulation, organogenesis, disease modeling, biomaterials
- MeSH terms
- Cell Culture Techniques, Three Dimensional, Biocompatible Materials, Biomedical Engineering, Extracellular Matrix, Genetic Engineering, Humans, Neoplasms, Organoids, Stem Cells, Hydrogels
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
