The DOI matches its Crossref record; 46 of the 46 references it cites were found in a registry or an index.
CRISPR/Cas13a Powered Portable Electrochemiluminescence Chip for Ultrasensitive and Specific MiRNA Detection
Open accessAbstract
MicroRNAs (miRNAs) have been widely investigated as potential biomarkers for early clinical diagnosis of cancer. Developing an miRNA detection platform with high specificity, sensitivity, and exploitability is always necessary. Electrochemiluminescence (ECL) is an electrogenerated chemiluminescence technology that greatly decreases background noise and improves detection sensitivity. The development of a paper‐based ECL biosensor further makes ECL suitable for point‐of‐care detection. Recently, clustered regularly interspaced short palindromic repeats (CRISPR)/Cas13a as high‐fidelity, efficient, and programmable CRISPR RNA (crRNA) guided RNase has brought a next‐generation biosensing technology. However, existing CRISPR/Cas13a based detection often faces a trade‐off between sensitivity and specificity. In this research, a CRISPR/Cas13a powered portable ECL chip (PECL‐CRISPR) is constructed. Wherein target miRNA activates Cas13a to cleave a well‐designed preprimer, and triggers the subsequent exponential amplification and ECL detection. Under optimized conditions, a limit‐of‐detection of 1 × 10−15 m for miR‐17 is achieved. Through rationally designing the crRNA, the platform can provide single nucleotide resolution to dramatically distinguish miRNA target from its highly homologous family members. Moreover, the introduction of “light‐switch” molecule [Ru(phen)2 dppz]2+ allows the platform to avoid tedious electrode modification and washing processes, thereby simplifying the experimental procedure and lower testing cost. Analysis results of miRNA from tumor cells also demonstrate the PECL‐CRISPR platform holds a promising potential for molecular diagnosis.
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Cites
A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity
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CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity
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Nucleic acid detection with CRISPR-Cas13a/C2c2
Gootenberg, J. S., Abudayyeh, O. O., Lee, J. W., Essletzbichler, P., Dy, A. J., Joung, J., Verdine, V., Donghia, N., Daringer, N. M., Freije, C. A., et al. (2017) · Science · doi:10.1126/science.aam9321 · Cited by 3,844
Bartel, D. P. (2018) · Cell · doi:10.1016/j.cell.2018.03.006 · Cited by 3,751
Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6
Gootenberg, J. S., Abudayyeh, O. O., Kellner, M. J., Joung, J., Collins, J. J., & Zhang, F. (2018) · Science · doi:10.1126/science.aaq0179 · Cited by 2,638
Electrochemiluminescence (ECL)
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An updated evolutionary classification of CRISPR–Cas systems
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C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector
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Cited by
Kaminski, M. M., Abudayyeh, O. O., Gootenberg, J. S., Zhang, F., & Collins, J. J. (2021) · Nature Biomedical Engineering · doi:10.1038/s41551-021-00760-7 · Cited by 1,259
Amplification-free CRISPR/Cas detection technology: challenges, strategies, and perspectives
Li, H., Xie, Y., Chen, F., Bai, H., Xiu, L., Zhou, X., Guo, X., Hu, Q., & Yin, K. (2023) · Chemical Society Reviews · doi:10.1039/d2cs00594h · Cited by 346
Accelerated RNA detection using tandem CRISPR nucleases
Liu, T. Y., Knott, G. J., Smock, D. C. J., Desmarais, J. J., Son, S., Bhuiya, A., Jakhanwal, S., Prywes, N., Agrawal, S., Díaz de León Derby, M., et al. (2021) · Nature Chemical Biology · doi:10.1038/s41589-021-00842-2 · Cited by 268
Repurposing Type I-A CRISPR-Cas3 for a robust diagnosis of human papillomavirus (HPV)
Hu, T., Ji, Q., Ke, X., Zhou, H., Zhang, S., Ma, S., Yu, C., Ju, W., Lu, M., Lin, Y., et al. (2024) · Communications Biology · doi:10.1038/s42003-024-06537-3 · Cited by 24
Metadata
- Authors
- Zhou, T., Huang, R., Huang, M., Shen, J., Shan, Y., & Xing, D.
- Venue
- Advanced Science
- Publisher
- Wiley
- Published
- 2020-05-27
- Volume
- 7
- Issue
- 13
- Pages
- 1903661-1903661
- ISSN
- 2198-3844 (ISSN-L)
- OpenAlex
- W3028883492
- Cited by
- 292
- References
- 46
- FWCI
- 11.82
- Type
- journal article
- Language
- en
- Open access
- gold
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- Zhou, T., Huang, R., Huang, M., Shen, J., Shan, Y., & Xing, D. (2020). CRISPR/Cas13a Powered Portable Electrochemiluminescence Chip for Ultrasensitive and Specific MiRNA Detection. Advanced Science. https://doi.org/10.1002/advs.201903661
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