The DOI matches its Crossref record.
Neuroblastoma × spinal cord (NSC) hybrid cell lines resemble developing motor neurons
Abstract
We have developed a series of mouse‐mouse neural hybrid cell lines by fusing the aminopterin‐sensitive neuroblastoma N18TG2 with motor neuron‐enriched embryonic day 12–14 spinal cord cells. Of 30 neuroblastoma‐spinal cord (NSC) hybrids displaying a multipolar neuron‐like phenotype, 10 express choline acetyltransferase, and 4 induce twitching in cocultured mouse myotubules. NSC‐19, NSC‐34, and their subclones express additional properties expected of motor neurons, including generation of action potentials, expression of neurofilament triplet proteins, and acetylcholine synthesis, storage, and release. In addition, NSC‐34 cells induce acetylcholine receptor clusters on cocultured myotubes, and undergo a vimentin‐neurofilament switch with maturation in culture, similar to that occurring in neuronal development. NSC cell lines appear to model selected aspects of motor neuron development in an immortalized clonal system. © 1992 Wiley‐Liss, Inc.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cited by
Oosthuyse, B., Moons, L., Storkebaum, E., Beck, H., Nuyens, D., Brusselmans, K., Dorpe, J. V., Hellings, P., Gorselink, M., Heymans, S., et al. (2001) · Nature Genetics · doi:10.1038/88842 · Cited by 1,007
Self-citationRusmini, P., Cortese, K., Crippa, V., Cristofani, R., Cicardi, M. E., Ferrari, V., Vezzoli, G., Tedesco, B., Meroni, M., Messi, E., et al. (2019) · Autophagy · doi:10.1080/15548627.2018.1535292 · Cited by 436
Grad, L. I., Yerbury, J. J., Turner, B. J., Guest, W. C., Pokrishevsky, E., O’Neill, M. A., Yanai, A., Silverman, J. M., Zeineddine, R., Corcoran, L., et al. (2014) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1312245111 · Cited by 414
Self-citationCrippa, V., Sau, D., Rusmini, P., Boncoraglio, A., Onesto, E., Bolzoni, E., Galbiati, M., Fontana, E., Marino, M., Carra, S., et al. (2010) · Human Molecular Genetics · doi:10.1093/hmg/ddq257 · Cited by 330
Intravenous scAAV9 delivery of a codon-optimized SMN1 sequence rescues SMA mice
Dominguez, E., Marais, T., Chatauret, N., Benkhelifa-Ziyyat, S., Duque, S., Ravassard, P., Carcenac, R., Astord, S., de Moura, A. P., Voit, T., et al. (2011) · Human Molecular Genetics · doi:10.1093/hmg/ddq514 · Cited by 298
Decreased levels of proteasome activity and proteasome expression in aging spinal cord
Keller, J. N., Huang, F. F., & Markesbery, W. R. (2000) · Neuroscience · doi:10.1016/s0306-4522(00)00067-1 · Cited by 297
In vivo genome editing improves motor function and extends survival in a mouse model of ALS
Gaj, T., Ojala, D. S., Ekman, F. K., Byrne, L. C., Limsirichai, P., & Schaffer, D. V. (2017) · Science Advances · doi:10.1126/sciadv.aar3952 · Cited by 185
Regulation of Autophagy by Neuropathological Protein TDP-43
Bose, J. K., Huang, C.-C., & Shen, C.-K. J. (2011) · Journal of Biological Chemistry · doi:10.1074/jbc.m111.237115 · Cited by 165
Goode, A., Butler, K., Long, J., Cavey, J., Scott, D., Shaw, B., Sollenberger, J., Gell, C., Johansen, T., Oldham, N. J., et al. (2016) · Autophagy · doi:10.1080/15548627.2016.1170257 · Cited by 153
Pinto, S., Cunha, C., Barbosa, M., Vaz, A. R., & Brites, D. (2017) · Frontiers in Neuroscience · doi:10.3389/fnins.2017.00273 · Cited by 135
Cytoplasmic accumulation and aggregation of TDP-43 upon proteasome inhibition in cultured neurons.
van Eersel, J., Ke, Y. D., Gladbach, A., Bi, M., Götz, J., Kril, J. J., & Ittner, L. M. (2011) · PLoS ONE · doi:10.1371/journal.pone.0022850 · Cited by 130
Development and characterisation of a glutamate-sensitive motor neurone cell line
Eggett, C. J., Crosier, S., Manning, P., Cookson, M. R., Menzies, F. M., McNeil, C. J., & Shaw, P. J. (2000) · Journal of Neurochemistry · doi:10.1046/j.1471-4159.2000.0741895.x · Cited by 111
Differentiated NSC-34 motoneuron-like cells as experimental model for cholinergic neurodegeneration
Maier, O., Böhm, J., Dahm, M., Brück, S., Beyer, C., & Johann, S. (2013) · Neurochemistry International · doi:10.1016/j.neuint.2013.03.008 · Cited by 108
Sayyad, Z., Sirohi, K., Radha, V., & Swarup, G. (2017) · Scientific Reports · doi:10.1038/s41598-017-17241-0 · Cited by 96
Crippa, V., D’Agostino, V. G., Cristofani, R., Rusmini, P., Cicardi, M. E., Messi, E., Loffredo, R., Pancher, M., Piccolella, M., Galbiati, M., et al. (2016) · Scientific Reports · doi:10.1038/srep22827 · Cited by 101
Pathogenic role of BECN1/Beclin 1 in the development of amyotrophic lateral sclerosis
Nassif, M., Valenzuela, V., Rojas-Rivera, D., Vidal, R., Matus, S., Castillo, K., Fuentealba, Y., Kroemer, G., Levine, B., & Hetz, C. (2014) · Autophagy · doi:10.4161/auto.28784 · Cited by 100
5α-Reductase isozymes in the central nervous system
Poletti, A., Coscarella, A., Negri-Cesi, P., Colciago, A., Celotti, F., & Martini, L. (1998) · Steroids · doi:10.1016/s0039-128x(98)00018-x · Cited by 100
Montagner, S., Leoni, C., Emming, S., Della Chiara, G., Balestrieri, C., Barozzi, I., Piccolo, V., Togher, S., Ko, M., Rao, A., et al. (2016) · Cell Reports · doi:10.1016/j.celrep.2016.04.044 · Cited by 93
Lee, A. W. S., Hengstler, H., Schwald, K., Berriel-Diaz, M., Loreth, D., Kirsch, M., Kretz, O., Haas, C. A., de Angelis, M. H., Herzig, S., et al. (2012) · PLoS ONE · doi:10.1371/journal.pone.0041537 · Cited by 84
Zhou, Y., Tang, J., Lan, J., Zhang, Y., Wang, H., Chen, Q., Kang, Y., Sun, Y., Feng, X., Wu, L., et al. (2023) · Acta Pharmaceutica Sinica B · doi:10.1016/j.apsb.2022.07.019 · Cited by 80
Superoxide dismutase 1 encoding mutations linked to ALS adopts a spectrum of misfolded states
Prudencio, M., & Borchelt, D. R. (2011) · Molecular Neurodegeneration · doi:10.1186/1750-1326-6-77 · Cited by 63
Characterization and use of the NSC-34 cell line for study of neurotrophin receptor trafficking
Matusica, D., Fenech, M. P., Rogers, M., & Rush, R. A. (2008) · Journal of Neuroscience Research · doi:10.1002/jnr.21507 · Cited by 67
Motor neuron-derived microRNAs cause astrocyte dysfunction in amyotrophic lateral sclerosis
Hoye, M. L., Regan, M. R., Jensen, L. A., Lake, A. M., Reddy, L. V., Vidensky, S., Richard, J.-P., Maragakis, N. J., Rothstein, J. D., Dougherty, J. D., et al. (2018) · Brain · doi:10.1093/brain/awy182 · Cited by 63
Tinnes, S., Schäfer, M. K. E., Flubacher, A., Münzner, G., Frotscher, M., & Haas, C. A. (2011) · The FASEB Journal · doi:10.1096/fj.10-168294 · Cited by 58
Li, C., Chen, Y., Chen, X., Wei, Q., Cao, B., & Shang, H. (2017) · Frontiers in Molecular Neuroscience · doi:10.3389/fnmol.2017.00160 · Cited by 45
“However, mTORC1 signaling coordinately activates anabolic processes, such as protein synthesis, while inhibiting the cellular catabolism of autophagy (Chan, 2009).”
BackgroundNemo-like kinase is a novel regulator of spinal and bulbar muscular atrophy
Todd, T. W., Kokubu, H., Miranda, H. C., Cortes, C. J., La Spada, A. R., & Lim, J. (2015) · eLife · doi:10.7554/elife.08493 · Cited by 21
Metadata
- Authors
- Cashman, N. R., Durham, H. D., Blusztajn, J. K., Oda, K., Tabira, T., Shaw, I. T., Dahrouge, S., & Antel, J. P.
- Venue
- Developmental Dynamics
- Publisher
- Wiley
- Published
- 1992-07
- Volume
- 194
- Issue
- 3
- Pages
- 209-221
- ISSN
- 1058-8388 (ISSN-L)
- 1097-0177
- OpenAlex
- W2010233410
- Cited by
- 722
- References
- 0
- FWCI
- 1.82
- Type
- journal article
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
