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Photon‐Upconverting Nanoparticles for Optical Encoding and Multiplexing of Cells, Biomolecules, and Microspheres
Abstract
Photon‐upconverting nanoparticles (UCNPs) are lanthanide‐doped nanocrystals that emit visible light under near‐infrared excitation (anti‐Stokes emission). This unique optical property precludes background fluorescence and light scattering from biological materials. The emission of multiple and narrow emission lines is an additional hallmark of UCNPs that opens up new avenues for optical encoding. Distinct emission signatures can be obtained if the multiple emission of UCNPs is tuned by their dopant composition or by surface modification with dyes. Tuning the intensity of only one of the multiple emission lines and using another one as a constant reference signal enables the design of ratiometric codes that are resistant to fluctuations in absolute signal intensities. Combining several UCNPs each displaying a distinct set of emission lines expands the coding capacity exponentially and lays the foundation for highly multiplexed analyte detection. This Review highlights the potential of UCNPs for labeling and encoding biomolecules, microspheres, and even whole cells.
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Longmire, M., Choyke, P. L., & Kobayashi, H. (2008) · Nanomedicine · doi:10.2217/17435889.3.5.703 · Cited by 2,149
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Wolfbeis, O. S. (2015) · Chemical Society Reviews · doi:10.1039/c4cs00392f · Cited by 1,631
Self-citationZheng, W., Huang, P., Tu, D., Ma, E., Zhu, H., & Chen, X. (2015) · Chemical Society Reviews · doi:10.1039/c4cs00178h · Cited by 894
Sun, L.-D., Wang, Y.-F., & Yan, C.-H. (2014) · Accounts of Chemical Research · doi:10.1021/ar400218t · Cited by 363
Rare-earths doped-nanoparticles prepared by pulsed laser ablation in liquids
Du, H., Castaing, V., Guo, D., & Viana, B. (2020) · Ceramics International · doi:10.1016/j.ceramint.2020.04.291 · Cited by 35
Metadata
- Authors
- Gorris, H. H., & Wolfbeis, O. S.
- Venue
- Angewandte Chemie International Edition
- Publisher
- Wiley
- Published
- 2013-02-28
- Volume
- 52
- Issue
- 13
- Pages
- 3584-3600
- ISSN
- 1433-7851 (ISSN-L)
- 1521-3773
- OpenAlex
- W2149095519
- Cited by
- 436
- References
- 1
- FWCI
- 27.38
- Type
- journal article
- Language
- en
- Open access
- closed
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- Gorris, H. H., & Wolfbeis, O. S. (2013). Photon‐Upconverting Nanoparticles for Optical Encoding and Multiplexing of Cells, Biomolecules, and Microspheres. Angewandte Chemie International Edition. https://doi.org/10.1002/anie.201208196
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