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Cited by 436

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Photon‐Upconverting Nanoparticles for Optical Encoding and Multiplexing of Cells, Biomolecules, and Microspheres

Gorris, Hans H., Wolfbeis, Otto S.·2013·Angewandte Chemie International Edition

Abstract

Photon‐upconverting nanoparticles (UCNPs) are lanthanide‐doped nanocrystals that emit visible light under near‐infrared excitation (anti‐Stokes emission). This unique optical property precludes background fluorescence and light scattering from biological materials. The emission of multiple and narrow emission lines is an additional hallmark of UCNPs that opens up new avenues for optical encoding. Distinct emission signatures can be obtained if the multiple emission of UCNPs is tuned by their dopant composition or by surface modification with dyes. Tuning the intensity of only one of the multiple emission lines and using another one as a constant reference signal enables the design of ratiometric codes that are resistant to fluctuations in absolute signal intensities. Combining several UCNPs each displaying a distinct set of emission lines expands the coding capacity exponentially and lays the foundation for highly multiplexed analyte detection. This Review highlights the potential of UCNPs for labeling and encoding biomolecules, microspheres, and even whole cells.

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Metadata

Authors
Gorris, H. H., & Wolfbeis, O. S.
Venue
Angewandte Chemie International Edition
Publisher
Wiley
Published
2013-02-28
Volume
52
Issue
13
Pages
3584-3600
ISSN
  • 1433-7851 (ISSN-L)
  • 1521-3773
OpenAlex
W2149095519
Cited by
436
References
1
FWCI
27.38
Type
journal article
Language
en
Open access
closed

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Gorris, H. H., & Wolfbeis, O. S. (2013). Photon‐Upconverting Nanoparticles for Optical Encoding and Multiplexing of Cells, Biomolecules, and Microspheres. Angewandte Chemie International Edition. https://doi.org/10.1002/anie.201208196

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