The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
Super‐Resolution Imaging of the Golgi in Live Cells with a Bioorthogonal Ceramide Probe
Open accessAbstract
We report a lipid‐based strategy to visualize Golgi structure and dynamics at super‐resolution in live cells. The method is based on two novel reagents: a trans‐cyclooctene‐containing ceramide lipid (Cer‐TCO) and a highly reactive, tetrazine‐tagged near‐IR dye (SiR‐Tz). These reagents assemble via an extremely rapid “tetrazine‐click” reaction into Cer‐SiR, a highly photostable “vital dye” that enables prolonged live‐cell imaging of the Golgi apparatus by 3D confocal and STED microscopy. Cer‐SiR is nontoxic at concentrations as high as 2 μ M and does not perturb the mobility of Golgi‐resident enzymes or the traffic of cargo from the endoplasmic reticulum through the Golgi and to the plasma membrane.
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NIH Image to ImageJ: 25 years of image analysis
Schneider, C. A., Rasband, W. S., & Eliceiri, K. W. (2012) · Nature Methods · doi:10.1038/nmeth.2089 · Cited by 64,638
Hell, S. W. (2007) · Science · doi:10.1126/science.1137395 · Cited by 2,967
Super-resolution fluorescence microscopy
Huang, B., Bates, M., & Zhuang, X. (2009) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.77.061906.092014 · Cited by 1,657
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Fluorescence nanoscopy in cell biology
Sahl, S. J., Hell, S. W., & Jakobs, S. (2017) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm.2017.71 · Cited by 990
Bioorthogonal Chemistry and Its Applications
Bird, R. E., Lemmel, S. A., Yu, X., & Zhou, Q. A. (2021) · Bioconjugate Chemistry · doi:10.1021/acs.bioconjchem.1c00461 · Cited by 409
Switchable Fluorophores for Single-Molecule Localization Microscopy
Li, H., & Vaughan, J. C. (2018) · Chemical Reviews · doi:10.1021/acs.chemrev.7b00767 · Cited by 319
SiR–Hoechst is a far-red DNA stain for live-cell nanoscopy
Lukinavičius, G., Blaukopf, C., Pershagen, E., Schena, A., Reymond, L., Derivery, E., Gonzalez-Gaitan, M., D’Este, E., Hell, S. W., Wolfram Gerlich, D., et al. (2015) · Nature Communications · doi:10.1038/ncomms9497 · Cited by 294
Metadata
- Authors
- Erdmann, R. S., Takakura, H., Thompson, A. D., Rivera‐Molina, F., Allgeyer, E. S., Bewersdorf, J., Toomre, D., & Schepartz, A.
- Venue
- Angewandte Chemie International Edition
- Publisher
- Wiley
- Published
- 2014-07-31
- Volume
- 53
- Issue
- 38
- Pages
- 10242-10246
- ISSN
- 1433-7851 (ISSN-L)
- 1521-3773
- OpenAlex
- W2096156293
- Cited by
- 164
- References
- 3
- FWCI
- 8.13
- Type
- journal article
- Language
- en
- Open access
- green
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- Erdmann, R. S., Takakura, H., Thompson, A. D., Rivera‐Molina, F., Allgeyer, E. S., Bewersdorf, J., Toomre, D., & Schepartz, A. (2014). Super‐Resolution Imaging of the Golgi in Live Cells with a Bioorthogonal Ceramide Probe. Angewandte Chemie International Edition. https://doi.org/10.1002/anie.201403349
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