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Cited by 1,657Advanced Fluorescence Microscopy TechniquesCell Image Analysis TechniquesForce Microscopy Techniques and Applications

Super-resolution fluorescence microscopy

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Huang, Bo, Bates, Mark, Zhuang, Xiaowei·2009·Annual Review of Biochemistry
DOI 10.1146/annurev.biochem.77.061906.092014PMC PMC2835776
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Abstract

Achieving a spatial resolution that is not limited by the diffraction of light, recent developments of super-resolution fluorescence microscopy techniques allow the observation of many biological structures not resolvable in conventional fluorescence microscopy. New advances in these techniques now give them the ability to image three-dimensional (3D) structures, measure interactions by multicolor colocalization, and record dynamic processes in living cells at the nanometer scale. It is anticipated that super-resolution fluorescence microscopy will become a widely used tool for cell and tissue imaging to provide previously unobserved details of biological structures and processes.

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Crossref · 10.1146/annurev.biochem.77.061906.092014

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  • SMLM uses switchable fluorophores or special imaging conditions to ensure that only a small, random subset of fluorophores in the sample is emitting at any given time.

    “Another set of powerful techniques to achieve resolutions beyond the diffraction barrier uses switchable fluorophores or special imaging conditions to ensure that only a small, random subset of fluorophores in the sample is emitting at any given time (Huang et al., 2009).”

    From Barriers in the brain: resolving dendritic spine morphology and compartmentalization (2014) · cites this paper as Huang et al., 2009

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Authors
Huang, B., Bates, M., & Zhuang, X.
Affiliations
  • Howard Hughes Medical Institute (US)
  • Harvard University (US)
ORCID
Venue
Annual Review of Biochemistry
Publisher
Annual Reviews
Published
2009-06-01
Volume
78
Issue
1
Pages
993-1016
ISSN
  • 0066-4154 (ISSN-L)
  • 1545-4509
DOI
10.1146/annurev.biochem.77.061906.092014
PMC
PMC2835776
OpenAlex
W2168816122
Cited by
1,657
References
0
Citations by year
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FWCI
10.48
Type
journal article
Language
en
Open access
Locations
  • Annual Review of Biochemistry (publishedVersion)
  • PubMed (publishedVersion)
  • PubMed Central (acceptedVersion)
Funders
  • Howard Hughes Medical Institute
  • National Institute of General Medical Sciences (R01GM068518)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Advanced Fluorescence Microscopy Techniques
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Cell Image Analysis Techniques
  • Physical Sciences › Physics and Astronomy › Atomic and Molecular Physics, and Optics › Force Microscopy Techniques and Applications
Keywords
super-resolution microscopy, fluorescence microscopy, diffraction limit, 3D imaging, live-cell imaging, nanoscale imaging, cell imaging, tissue imaging, biological structures
MeSH terms
Cytological Techniques, Humans, Microscopy, Fluorescence, Imaging, Three-Dimensional

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