The DOI matches its Crossref record; 4 of the 4 references it cites were found in a registry or an index.
Advances and Challenges in Small‐Molecule DNA Aptamer Isolation, Characterization, and Sensor Development
Open accessAbstract
Aptamers are short oligonucleotides isolated in vitro from randomized libraries that can bind to specific molecules with high affinity, and offer a number of advantages relative to antibodies as biorecognition elements in biosensors. However, it remains difficult and labor‐intensive to develop aptamer‐based sensors for small‐molecule detection. Here, we review the challenges and advances in the isolation and characterization of small‐molecule‐binding DNA aptamers and their use in sensors. First, we discuss in vitro methodologies for the isolation of aptamers, and provide guidance on selecting the appropriate strategy for generating aptamers with optimal binding properties for a given application. We next examine techniques for characterizing aptamer–target binding and structure. Afterwards, we discuss various small‐molecule sensing platforms based on original or engineered aptamers, and their detection applications. Finally, we conclude with a general workflow to develop aptamer‐based small‐molecule sensors for real‐world applications.
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Cites
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Aptamer-Based Multiplexed Proteomic Technology for Biomarker Discovery
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Cited by
McConnell, E. M., Cozma, I., Mou, Q., Brennan, J. D., Lu, Y., & Li, Y. (2021) · Chemical Society Reviews · doi:10.1039/d1cs00240f · Cited by 477
Biomolecular sensors for advanced physiological monitoring
Flynn, C. D., Chang, D., Mahmud, A., Yousefi, H., Das, J., Riordan, K. T., Sargent, E. H., & Kelley, S. O. (2023) · Nature Reviews Bioengineering · doi:10.1038/s44222-023-00067-z · Cited by 226
Son, N., & Hong, S. (2025) · Materials Today · doi:10.1016/j.mattod.2025.06.021 · Cited by 9
Metadata
- Authors
- Yu, H., Alkhamis, O., Canoura, J., Liu, Y., & Xiao, Y.
- Venue
- Angewandte Chemie International Edition
- Publisher
- Wiley
- Published
- 2021-02-09
- Volume
- 60
- Issue
- 31
- Pages
- 16800-16823
- ISSN
- 1433-7851 (ISSN-L)
- 1521-3773
- OpenAlex
- W3126685531
- Cited by
- 492
- References
- 4
- FWCI
- 18.52
- Type
- journal article
- Language
- en
- Open access
- green
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- Yu, H., Alkhamis, O., Canoura, J., Liu, Y., & Xiao, Y. (2021). Advances and Challenges in Small‐Molecule DNA Aptamer Isolation, Characterization, and Sensor Development. Angewandte Chemie International Edition. https://doi.org/10.1002/anie.202008663
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