Degradation of poly(L‐lactide) by a mesophilic bacterium
Abstract
Abstract A Gram negative, rod‐shaped mesophilic bacterium active for poly(L‐lactide) (PLA) degradation was isolated through the enrichment culture and clear‐zone method. The isolated strain was identified to be Bordetella petrii PLA‐3 on the basis of 16S rDNA gene sequence analysis. B. petrii PLA‐3 was active not only for the degradation of low‐molecular‐weight PLA but also for the degradation of high‐molecular‐weight PLA. The strain seemed to attack the crystalline part of PLA as well as the amorphous region. The PLA film incubated in compost inoculated with the isolated strain lost its weight more notably and exhibited a lower molecular weight than that incubated in the sterilized compost without living microorganisms. Moreover, the profile of the cumulative amount of CO2 after 20 days of burial in the sterilized compost and subsequent inoculation of the isolated strain into compost was nearly the same as that of CO2 evolved from PLA buried in compost with the isolated strain at the very beginning when the time was shifted by 20 days. This indicated that not only the abiotic hydrolysis but also the microbial enzymes of the strain contributed to the initial chain cleavage of PLA molecules and resolved the doubt that PLA molecules should be initially cleaved into very low‐molecular‐weight substances by abiotic hydrolysis to be subsequently absorbed into and biodegraded by microorganisms. © 2010 Wiley Periodicals, Inc. J Appl Polym Sci, 2010
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At higher crystallinity degrees, biodegradation takes more time because chains in crystalline regions are highly ordered, making them more compact and less mobile, which makes it more difficult for biodegrading agents to access polymeric chains.
“At higher crystallinity degrees, the biodegradation takes more time because the chains in the crystalline regions are highly ordered, making them more compact and less mobile, which makes it more difficult for biodegrading agents to access the polymeric chains.”
Lower crystallinity results in a faster biodegradation rate.
“studies of PLAs with different crystallinities confirmed that lower crystallinity resulted in a faster biodegradation rate [44].”
Biodegradation rates decrease as molecular weight increases.
“The authors reported that biodegradation rates decreased as molecular weight increased.”
Cites
Thompson, J. D., Higgins, D. G., & Gibson, T. J. (1994) · Nucleic Acids Research · doi:10.1093/nar/22.22.4673 · Cited by 64,617 · see citing works
The neighbor-joining method: a new method for reconstructing phylogenetic trees.
Saitou, N., & Nei, M. (1987) · Molecular Biology and Evolution · doi:10.1093/oxfordjournals.molbev.a040454 · Cited by 59,962 · see citing works
16S ribosomal DNA amplification for phylogenetic study
Weisburg, W. G., Barns, S. M., Pelletier, D. A., & Lane, D. J. (1991) · Journal of Bacteriology · doi:10.1128/jb.173.2.697-703.1991 · Cited by 11,970 · see citing works
MEGA3: Integrated software for Molecular Evolutionary Genetics Analysis and sequence alignment
Kumar, S. (2004) · Briefings in Bioinformatics · doi:10.1093/bib/5.2.150 · Cited by 11,642 · see citing works
Fischer, E. W., Sterzel, H. J., & Wegner, G. (1973) · Kolloid-Zeitschrift und Zeitschrift für Polymere · doi:10.1007/bf01498927 · Cited by 1,704 · see citing works
Polylactide Degradation by an Amycolatopsis sp
Pranamuda, H., Tokiwa, Y., & Tanaka, H. (1997) · Applied and Environmental Microbiology · doi:10.1128/aem.63.4.1637-1640.1997 · Cited by 288 · see citing works
Dependence of biodegradability of plastics in compost on the shape of specimens
Yang, H.-S., Yoon, J., & Kim, M.-N. (2004) · Polymer Degradation and Stability · doi:10.1016/j.polymdegradstab.2004.07.016 · Cited by 154 · see citing works
Self-citationNakamura, K., Tomita, N., Abe, N., & Kamio, Y. (2001) · Applied and Environmental Microbiology · doi:10.1128/aem.67.1.345-353.2001 · Cited by 150 · see citing works
Hoshino, A., & Isono, Y. (2002) · Biodegradation · doi:10.1023/a:1020450326301 · Cited by 146 · see citing works
Torres, A., Li, S., Roussos, S., & Vert, M. (1996) · Applied and Environmental Microbiology · doi:10.1128/aem.62.7.2393-2397.1996 · Cited by 136 · see citing works
Teeraphatpornchai, T., Nakajima‐Kambe, T., Akutsu, Y., Nakayama, M., Nomura, N., Nakahara, T., & Uchiyama, H. (2003) · Biotechnology Letters · doi:10.1023/a:1021713711160 · Cited by 133 · see citing works
Degradation of poly(L-lactide) by strains belonging to genus Amycolatopsis
Pranamuda, H., & Tokiwa, Y. (1999) · Biotechnology Letters · doi:10.1023/a:1005547326434 · Cited by 113 · see citing works
Akutsu, Y., Teeraphatpornchai, T., Teamtisong, K., Nomura, N., Uchiyama, H., Nakahara, T., & Nakajima‐Kambe, T. (2003) · Applied and Environmental Microbiology · doi:10.1128/aem.69.5.2498-2504.2003 · Cited by 110 · see citing works
Poly( L ‐lactide)‐Degrading Activity in Various Actinomycetes
Jarerat, A., Pranamuda, H., & Tokiwa, Y. (2002) · Macromolecular Bioscience · doi:10.1002/mabi.200290001 · Cited by 107 · see citing works
Isolation of thermophiles degrading poly(l-lactic acid)
Tomita, K., Kuroki, Y., & Nagai, K. (1999) · Journal of Bioscience and Bioengineering · doi:10.1016/s1389-1723(99)80148-0 · Cited by 99 · see citing works
Uchida, H., Nakajima‐Kambe, T., Akutsu, Y., Nomura, N., Tokiwa, Y., & Nakahara, T. (2000) · FEMS Microbiology Letters · doi:10.1111/j.1574-6968.2000.tb09201.x · Cited by 97 · see citing works
Sakai, K., Kawano, H., Iwami, A., Nakamura, M., & Moriguchi, M. (2001) · Journal of Bioscience and Bioengineering · doi:10.1016/s1389-1723(01)80266-8 · Cited by 97 · see citing works
Wang, F., Grundmann, S., Schmid, M., Dörfler, U., Roherer, S., Munch, J. C., Hartmann, A., Jiang, X., & Schroll, R. (2007) · Chemosphere · doi:10.1016/j.chemosphere.2006.11.019 · Cited by 70 · see citing works
Degradation of poly(l-lactic acid) by a newly isolated thermophile
Tomita, K., Nakajima, T., Kikuchi, Y., & Miwa, N. (2004) · Polymer Degradation and Stability · doi:10.1016/j.polymdegradstab.2003.12.006 · Cited by 58 · see citing works
Effects of storage of a mature compost on its potential for biodegradation of plastics
Yang, H.-S., Yoon, J., & Kim, M.-N. (2004) · Polymer Degradation and Stability · doi:10.1016/j.polymdegradstab.2004.01.014 · Cited by 54 · see citing works
Self-citationMicrobial degradation of poly-(l-lactic acid) oligomers
Karjomaa, S., Suortti, T., Lempiäinen, R., Selin, J., & Itävaara, M. (1998) · Polymer Degradation and Stability · doi:10.1016/s0141-3910(97)00204-8 · Cited by 50 · see citing works
Poly( L ‐lactide)‐degrading activity of a newly isolated bacterium
Kim, M.-N., Kim, W. G., Weon, H., & Lee, S. H. (2008) · Journal of Applied Polymer Science · doi:10.1002/app.26658 · Cited by 26 · see citing works
Self-citationBianchi, F., Careri, M., Mustat, L., Malcevschi, A., & Musci, M. (2005) · Annali di Chimica · doi:10.1002/adic.200590061 · Cited by 19 · see citing works
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Lu, J., Zhang, L., Wang, J., Li, G., Qiu, Y., Ren, L., & Wang, P. (2024) · Environmental Reviews · doi:10.1139/er-2024-0058 · Cited by 20 · see citing works
Khoshtinat, S. (2023) · Corrosion and Materials Degradation · doi:10.3390/cmd4040029 · Cited by 20 · see citing works
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- Authors
- Kim, M. N., & Park, S. T.
- Affiliations
- Sangmyung University (KR)
- Corresponding author
- Kim, Mal Nam
- Venue
- Journal of Applied Polymer Science
- Publisher
- Wiley
- Published
- 2010-03-30
- Volume
- 117
- Issue
- 1
- Pages
- 67-74
- ISSN
- 0021-8995 (ISSN-L)
- 1097-4628
- OpenAlex
- W2072238447
- Cited by
- 51
- References
- 23
- Citations by year
- 2026: 3 · 2025: 1 · 2024: 7 · 2023: 7 · 2022: 4 · 2021: 6 · 2020: 4 · 2019: 4 · 2018: 2 · 2017: 2 · 2016: 5 · 2015: 3 · 2014: 2 · 2012: 1
- FWCI
- 0.13
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Applied Polymer Science (publishedVersion)
- Topics
- Physical Sciences › Materials Science › Biomaterials › biodegradable polymer synthesis and properties
- Physical Sciences › Environmental Science › Pollution › Microbial bioremediation and biosurfactants
- Physical Sciences › Environmental Science › Pollution › Microplastics and Plastic Pollution
- Keywords
- poly(L-lactic acid), biodegradation, Bordetella petrii, mesophilic bacteria, biodegradable plastics, compost, abiotic hydrolysis, chain cleavage, 16S rRNA identification, bioplastic waste
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