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Cited by 26Alkaline Phosphatase Research StudiesOsteoarthritis Treatment and MechanismsBone and Dental Protein Studies

Mineralized spherules in the cells and matrix of calcifying cartilage from developing bone

Kashiwa, Herbert K., Komorous, James·1971·The Anatomical Record
DOI 10.1002/ar.1091700109
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Abstract

Fresh tibias and occipital bones from newborn mice and rats, as well as from 18‐day‐old mouse and chick embryos, were stained with 10% GBHA and 5% AgNO 3 solutions to localize calcium and phosphate or carbonates in sphenules within the cytoplasm of chondrocytes and in the matrix of the calcifying cartilages. The mineralized spherules in the matrix adjacent to the hypertrophied chondrocytes were approximately 0.5 to 1 μ in diameter. This was comparable in size to the spherules found within the cytoplasm of the chondrocytes. The spherules within the core of the spicules distal to the hypertrophied chondrocytes were about 2–3 μ in diameter. The larger spherules were shown to contain an organic core which stained metachromatically with toluidine blue and was also stained with aldehyde fuchsin. The spherules were not visible after PAS reactions due to the intense staining. The labile GBHA and silver acetate positive spherules localized in the chondrocytes may be the source of the extracellular mineralized spherules.

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  • Intra- and extracellular apatitic granules were observed in calcifying cartilage, baleen, atremate brachiopods, Lingula adamsi and Glottidia pyramidata, and the protozoan Spirostomum ambiguum.

    “Intra- and extracellular apatitic granules, which have also been described by alternate names such as “spherules,” “spherulites,” “microspheres,” and “calcospherites,” were observed in calcifying cartilage [44–48, 118, 134–137], baleen [138], invertebrates such as the atremate brachiopods [45, 139], Lingula adamsi and Glottidia pyramidata [46], and the protozoa Spirostomum ambiguum [47, 140].”

    From A Review of Phosphate Mineral Nucleation in Biology and Geobiology (2013) · cites this paper as [135]

  • Kashiwa and Komorous demonstrated intra- and extracellular calcium- and P-rich spherules within fresh calcifying cartilage samples from regions preceding endochondral calcification.

    “Kashiwa and Komorous [135] demonstrated intra- and extracellular calcium- and P-rich spherules within fresh calcifying cartilage samples from regions preceding endochondral calcification.”

    From A Review of Phosphate Mineral Nucleation in Biology and Geobiology (2013) · cites this paper as [135]

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Authors
Kashiwa, H. K., & Komorous, J.
Affiliations
University of Washington (US)
Venue
The Anatomical Record
Publisher
Wiley
Published
1971-05
Volume
170
Issue
1
Pages
119-127
ISSN
  • 0003-276X (ISSN-L)
  • 1097-0185
DOI
10.1002/ar.1091700109
OpenAlex
W1986782194
Cited by
26
References
22
Citations by year
2025: 1 · 2020: 1 · 2014: 1 · 2013: 1
FWCI
1.22
Type
journal article
Language
en
Open access
closed
Licence
onlinelibrary.wiley.com/termsAndConditions#vor (vor, 12325 d)
Locations
  • The Anatomical Record (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Alkaline Phosphatase Research Studies
  • Health Sciences › Medicine › Rheumatology › Osteoarthritis Treatment and Mechanisms
  • Health Sciences › Medicine › Rheumatology › Bone and Dental Protein Studies
Keywords
chondrocytes, skeletal development, extracellular matrix mineralization, silver nitrate staining, spicules, embryonic bone, neonatal mice, chick embryo
MeSH terms
Animals, Animals, Newborn, Bone Development, Calcification, Physiologic, Calcium, Carbonates, Cartilage, Inclusion Bodies, Chick Embryo, Cytoplasmic Granules, Epiphyses, Histocytochemistry, Occipital Bone, Phosphates, Silver Nitrate, Staining and Labeling, Tibia, Mice, Rats

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