Morphology of aging lung in F344/N rat: Alveolar size, connective tissue, and smooth muscle cell markers
Open accessAbstract
This study investigated the morphological changes of lungs in F344/N rats (9–36 months old). We initially examined general and quantitative morphological changes, and then we used immunohistochemistry to detect distributional changes in collagen subtypes (types I, III, and IV) and smooth muscle cell (SMC) markers (α‐smooth muscle actin (ASMA), γ‐smooth muscle actin (GSMA), desmin, and vimentin) in the lungs. In 24‐month‐old rats, alveolar ducts and alveolar sacs were enlarged, and alveoli were wider and shallower than in younger animals. In old rats (≥27 months), terminal and respiratory bronchioles and alveolar ducts were dilated and alveoli were more extended than in 24‐month‐old rats. No age‐related distributional changes were observed for collagen types I, III, and IV as revealed by immunohistochemistry, or elastin as revealed by resorsin fuchsin. SMCs in the extra‐ and intrapulmonary bronchi were immunoreactive for ASMA, GSMA, and desmin, but not for vimentin at all ages. In old rats (≥27 months), SMCs were loosely arranged in comparison with younger animals, and stainability for GSMA and desmin was decreased. In the respiratory bronchioles and alveolar ducts, a few cells immunoreactive for ASMA and vimentin were observed in the smooth muscle aggregations of the alveolar orifice in rats younger than 12 months. In older rats (>20 months), cells immunoreactive for ASMA and vimentin were increased in septal tips. In conclusion, extension of distal airways and immunohistochemical changes of SMC markers in F344/N rat lungs were evident by ∼24 months of age, but there was no apparent change in connective tissue morphology. Anat Rec Part A 272A:538–547, 2003. © 2003 Wiley‐Liss, Inc.
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Increased α-smooth muscle actin and vimentin expression in the distal airway suggests that mechanical forces are strengthened in the peripheral airways.
“suggesting that mechanical forces are strengthened in the peripheral airways (100).”
From Aging and Lung Disease (2020) · cites this paper as [100]
α-smooth muscle actin and vimentin expression in the distal airway may be increased in aged lung.
“Interestingly, α-smooth muscle actin and vimentin expression in the distal airway may be increased in aged lung”
From Aging and Lung Disease (2020) · cites this paper as [100]
Experimental aging models have illustrated decreased expression of γ-smooth muscle actin and desmin in aged lung.
“Experimental aging models have illustrated decreased expression of γ-smooth muscle actin and desmin in aged lung”
From Aging and Lung Disease (2020) · cites this paper as [100]
Decreased expression of γ-smooth muscle actin and desmin in aged lung may indicate that contractions of larger intrapulmonary airways decline with aging.
“which may indicate that contractions of larger intrapulmonary airways decline with aging (100).”
From Aging and Lung Disease (2020) · cites this paper as [100]
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- Authors
- Yamamoto, Y., Tanaka, A., Kanamaru, A., Tanaka, S., Tsubone, H., Atoji, Y., & Suzuki, Y.
- Affiliations
- Gifu University (JP)
- The University of Tokyo (JP)
- Corresponding author
- Yamamoto, Yoshio
- Venue
- The Anatomical Record Part A: Discoveries in Molecular, Cellular, and Evolutionary Biology
- Publisher
- Wiley
- Published
- 2003-04-24
- Volume
- 272A
- Issue
- 2
- Pages
- 538-547
- ISSN
- 1552-4884 (ISSN-L)
- 1552-4892
- OpenAlex
- W1968206913
- Cited by
- 41
- References
- 22
- Citations by year
- 2026: 1 · 2024: 2 · 2023: 1 · 2022: 3 · 2021: 3 · 2020: 2 · 2019: 6 · 2018: 2 · 2017: 1 · 2016: 2 · 2015: 1 · 2014: 1 · 2013: 2 · 2012: 3
- FWCI
- 0.81
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- The Anatomical Record Part A Discoveries in Molecular Cellular and Evolutionary Biology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Chronic Obstructive Pulmonary Disease (COPD) Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Aging › Genetics, Aging, and Longevity in Model Organisms
- Keywords
- lung aging, alveolar size, smooth muscle cells, alpha-smooth muscle actin, desmin, vimentin, elastin, connective tissue
- MeSH terms
- Actins, Aging, Animals, Bronchi, Connective Tissue, Desmin, Elastin, Female, Immunohistochemistry, Male, Pulmonary Alveoli, Rats, Inbred F344, Vimentin, Biomarkers, Collagen Type I, Collagen Type III, Collagen Type IV, Myocytes, Smooth Muscle, Rats
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