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Single‐cell microinjection technology in cell biology
Abstract
Single‐cell microinjection has been successfully used to deliver exogenous proteins, cDNA constructs, peptides, drugs and particles into transfection‐challenged cells. With precisely controlled delivery dosage and timing, microinjection has been used in many studies of primary cultured cells, transgenic animal production, in vitro fertilization and RNA inference. This review discusses the advantages and limits of microinjection as a mechanical delivery method and its applications to attached and suspended cells. BioEssays 30:606–610, 2008. © 2008 Wiley Periodicals, Inc.
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“However, microinjection is an intrusive method that can damage the cell and requires the individual injection of each cell, thus constituting a laborious and time-consuming task [140]”
From CRISPR-Cas: Converting A Bacterial Defence Mechanism into A State-of-the-Art Genetic Manipulation Tool (2019) · cites this paper as [140]
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Zhang, Y., McLaughlin, R., Goodyer, C., & LeBlanc, A. (2002) · The Journal of Cell Biology · doi:10.1083/jcb.200110119 · Cited by 418
Self-citationGuo, H., Albrecht, S., Bourdeau, M., Petzke, T., Bergeron, C., & LeBlanc, A. C. (2004) · The American Journal of Pathology · doi:10.1016/s0002-9440(10)63317-2 · Cited by 287
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Cited by
Sorted by citations
Loureiro, A., & da Silva, G. J. (2019) · Antibiotics · doi:10.3390/antibiotics8010018 · Cited by 129
Metadata
- Authors
- Zhang, Y., & Yu, L.
- Venue
- BioEssays
- Publisher
- Wiley
- Published
- 2008-05-13
- Volume
- 30
- Issue
- 6
- Pages
- 606-610
- ISSN
- 0265-9247
- 1521-1878
- Cited by
- 134
- References
- 2
- Language
- en
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