One-step generation of triple knockout CHO cell lines using CRISPR/Cas9 and fluorescent enrichment

Grav, Lise Marie, Lee, Jae Seong, Gerling, Signe, Kallehauge, Thomas Beuchert, Hansen, Anders Holmgaard, Kol, Stefan, Lee, Gyun Min, Pedersen, Lasse Ebdrup +1 more·2015·Biotechnology Journal

Cited by (131)

Items 101–131 of 131

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  4. Towards a cleaner CHO chassis: systematic knockout of host cell proteins for efficient biopharmaceutical manufacturing
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  5. Rapid Antibody Glycoengineering in CHO Cells Via RNA Interference and CGE-LIF N-Glycomics
    Pavlos Kotidis, Masue M. Marbiah, Roberto Donini, Itzcóatl A. Gómez, Ioscani Jiménez del Val, Stuart M. Haslam, Karen Marie Polizzi, Cleo Kontoravdi · 2021 · Methods in molecular biology
  6. Integration of high‐throughput analytics and cell imaging enables direct early productivity and product quality assessment during Chinese Hamster ovary cell line development for a complex multi‐subunit vaccine antigen
    Xiangming Li, Yujian Zhang, Jing Li, Zongming Fu, Ou Ma, Jishna Ganguly, Nilesh Vaidya, Richard Sisson, Jennifer Naginskaya, Avinash Chinthala et al. · 2019 · Biotechnology Progress
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    Frances Rocamora, Chen‐Lin Hsieh, Sanne Schoffelen, Johnny Arnsdorf, Eric A. Toth, Abdul S. Yunus, Thomas E. Cleveland, Sara Petersen Bjørn, Mina Ying Min Wu, Noel Gerard McElvaney et al. · 2026 · Biotechnology and Bioengineering
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    Ching‐Hui Huang, Szu‐Ying Chiu, Yu‐Chi Chou, Kou‐Juey Wu · 2024 · Biotechnology Journal
  9. Synthetic biology approaches: the next tools for improved protein production from CHO cells
    Claire E. McGraw, Danqia Peng, Nicholas R. Sandoval · 2020 · Current Opinion in Chemical Engineering
  10. Recombinant Protein Drugs: A 2025 Update
    Hèctor López‐Laguna, Eloi Parladé, Marianna Teixeira de Pinho Favaro, Carlos Palacin, Eric Voltà‐Durán, Neus Ferrer‐Miralles, José Luís Corchero, Rosalía Rodríguez‐Rodríguez, Esther Vázquez, Ugutz Unzueta et al. · 2026 · BioDrugs
  11. Application of CRISPR/Cas9 Genome Editing to Improve Recombinant Protein Production in CHO Cells
    Lise Marie Grav, Johan Rojek, Karen Julie la Cour Karottki, Jae Seong Lee, Helene Faustrup Kildegaard · 2024 · Methods in molecular biology
  12. TGF-β-mediated Endothelial to Mesenchymal Transition (EndMT) and the Functional Assessment of EndMT Effectors using CRISPR/Cas9 Gene Editing
    Jin Ma, Gerard van der Zon, Gonzalo Sánchez‐Duffhues, Peter ten Dijke · 2021 · Journal of Visualized Experiments
  13. CRISPR Toolbox for Mammalian Cell Engineering
    Daria Sergeeva, Karen Julie la Cour Karottki, Jae Seong Lee, Helene Faustrup Kildegaard · 2019 · Wiley eBooks
  14. Identification of novel miRNA targets in CHO cell lines and characterization of their impact on protein N-glycosylation
    Ankita Singh, Yuzhou Fan, Selgin D. Cakal, Thomas Amann, Anders Holmgaard Hansen, Nicole Borth, Gyun Min Lee, Helene Faustrup Kildegaard, Mikael Rørdam Andersen · 2022
  15. Reporter Alleles in hiPSCs: Visual Cues on Development and Disease
    Gustavo Caldeira Cotta, Rachel Castro Teixeira dos Santos, Guilherme Mattos Jardim Costa, Samyra Maria dos Santos Nassif Lacerda · 2024 · International Journal of Molecular Sciences
  16. Antiapoptosis Engineering for Improved Protein Production from CHO Cells
    Eric Hyungseok Baek, Soo Min Noh, Su Hyun Kim, Gyun Min Lee · 2024 · Methods in molecular biology
  17. Enhancing Product and Bioprocess Attributes Using Genome‐Scale Models of CHO Metabolism
    Shangzhong Li, Anne Richelle, Nathan E. Lewis · 2019 · Wiley eBooks
  18. In Vitro Ovarian Model Systems
    Pj Devine, S. K. Petrillo, Rita G Cortvrindt, L. Rasmussen, Emma Paunil, Zelieann Rivera Craig · 2016 · Elsevier eBooks
  19. Geração de novas variantes de fator IX recombinante humano com aumento da sua atividade biológica
    Aline de Sousa Bomfim · 2018
  20. Genome-editing techniques to increase the therapeutic efficacy of monoclonal antibodies
    Victoria Valerievna Firstova, I. G. Shemyakin, Ivan Alekseevich Dyatlov · 2020 · Annals of the Russian academy of medical sciences
  21. Glycoengineering of Chinese Hamster Ovary cells for improving biotherapeutics’ efficacies.
    Cheng‐Yu Chung · 2016
  22. Genome Editing of Eukarya
    Jonathan Asmund Arnesen, Jakob Blæsbjerg Nielsen, Helene Faustrup Kildegaard, Irina Borodina · 2021 · Metabolic Engineering
  23. El trabajo de la autonomía de 3 a 6 años
    Dolors Blanch, Leonor March · 2013 · Aula de infantil
  24. Monoclonal Antibody Glycoengineering for Biopharmaceutical Quality Assurance
    Itzcóatl A. Gómez, Ioscani Jiménez del Val · 2021 · Cell engineering
  25. CRISPR-Cas13d for CHO Cell Engineering and Antibody Production
    Mei‐Wei Lin, Chih‐Che Shen, Yen-Ju Lin, Min‐Yuan Chou, Nam Ngoc Pham, Yi‐Hao Chang, Chin-Wei Chang, Jih Ru Hwu, Yu‐Chen Hu · 2022
  26. Correlating amino acid profiles with improved expression capability of superior CHO based expression platforms
    Vijeta Sharma, Richa Guleria, Krishna Jyoti Mukherjee · 2026 · Indian Journal of Biochemistry and Biophysics
  27. Fucosylated IgG Contributes to Adipose Tissue Dysfunction During Aging
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  28. STABILON enhances recombinant protein expression by mitigating proteolytic degradation in CHO cells
    Jiaxin Li, Ya-ru Zhao, Qiu-li Sun, Zimeng Han, Yanfang Wang, Xiao han Guo, Tianyun Wang, Tianyun Wang · 2025 · Biochemical Engineering Journal
  29. Generation of Long-Lived CHO Cells Suitable for Production of Afucosylated Antibodies and Fc-Fusion Proteins
    Denis Kolesov, Nadezhda A. Orlova, Ivan Ivanovich Vorobiev · 2025 · Doklady Biological Sciences
  30. Enhancing TGF-β1 production in CHO cells via MS-275 treatment, Bax/Bak knockout, and PACEsol overexpression
    Kyungsoo Kim, Kyoung Eun Baek, Gyun Min Lee · 2026 · Biotechnology and Bioprocess Engineering
  31. The historical evolution of CHO cell line development for biologics production: 60 years and still counting.
    Juergen Fieder, Stefan Schneider, Simon Fischer · 2026 · Critical Reviews in Biotechnology