One-step generation of triple knockout CHO cell lines using CRISPR/Cas9 and fluorescent enrichment
Grav, Lise Marie, Lee, Jae Seong, Gerling, Signe, Kallehauge, Thomas Beuchert, Hansen, Anders Holmgaard, Kol, Stefan, Lee, Gyun Min, Pedersen, Lasse Ebdrup +1 more·2015·Biotechnology Journal
Cited by (131)
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- Rapid Antibody Glycoengineering in CHO Cells Via RNA Interference and CGE-LIF N-GlycomicsPavlos Kotidis, Masue M. Marbiah, Roberto Donini, Itzcóatl A. Gómez, Ioscani Jiménez del Val, Stuart M. Haslam, Karen Marie Polizzi, Cleo Kontoravdi · 2021 · Methods in molecular biology
- Integration of high‐throughput analytics and cell imaging enables direct early productivity and product quality assessment during Chinese Hamster ovary cell line development for a complex multi‐subunit vaccine antigenXiangming Li, Yujian Zhang, Jing Li, Zongming Fu, Ou Ma, Jishna Ganguly, Nilesh Vaidya, Richard Sisson, Jennifer Naginskaya, Avinash Chinthala et al. · 2019 · Biotechnology Progress
- Glycoengineered Recombinant Alpha1‐Antitrypsin Results in Comparable In Vitro and In Vivo Activities to Human Plasma‐Derived ProteinFrances Rocamora, Chen‐Lin Hsieh, Sanne Schoffelen, Johnny Arnsdorf, Eric A. Toth, Abdul S. Yunus, Thomas E. Cleveland, Sara Petersen Bjørn, Mina Ying Min Wu, Noel Gerard McElvaney et al. · 2026 · Biotechnology and Bioengineering
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- Synthetic biology approaches: the next tools for improved protein production from CHO cellsClaire E. McGraw, Danqia Peng, Nicholas R. Sandoval · 2020 · Current Opinion in Chemical Engineering
- Recombinant Protein Drugs: A 2025 UpdateHèctor López‐Laguna, Eloi Parladé, Marianna Teixeira de Pinho Favaro, Carlos Palacin, Eric Voltà‐Durán, Neus Ferrer‐Miralles, José Luís Corchero, Rosalía Rodríguez‐Rodríguez, Esther Vázquez, Ugutz Unzueta et al. · 2026 · BioDrugs
- Application of CRISPR/Cas9 Genome Editing to Improve Recombinant Protein Production in CHO CellsLise Marie Grav, Johan Rojek, Karen Julie la Cour Karottki, Jae Seong Lee, Helene Faustrup Kildegaard · 2024 · Methods in molecular biology
- TGF-β-mediated Endothelial to Mesenchymal Transition (EndMT) and the Functional Assessment of EndMT Effectors using CRISPR/Cas9 Gene EditingJin Ma, Gerard van der Zon, Gonzalo Sánchez‐Duffhues, Peter ten Dijke · 2021 · Journal of Visualized Experiments
- CRISPR Toolbox for Mammalian Cell EngineeringDaria Sergeeva, Karen Julie la Cour Karottki, Jae Seong Lee, Helene Faustrup Kildegaard · 2019 · Wiley eBooks
- Identification of novel miRNA targets in CHO cell lines and characterization of their impact on protein N-glycosylationAnkita Singh, Yuzhou Fan, Selgin D. Cakal, Thomas Amann, Anders Holmgaard Hansen, Nicole Borth, Gyun Min Lee, Helene Faustrup Kildegaard, Mikael Rørdam Andersen · 2022
- Reporter Alleles in hiPSCs: Visual Cues on Development and DiseaseGustavo Caldeira Cotta, Rachel Castro Teixeira dos Santos, Guilherme Mattos Jardim Costa, Samyra Maria dos Santos Nassif Lacerda · 2024 · International Journal of Molecular Sciences
- Antiapoptosis Engineering for Improved Protein Production from CHO CellsEric Hyungseok Baek, Soo Min Noh, Su Hyun Kim, Gyun Min Lee · 2024 · Methods in molecular biology
- Enhancing Product and Bioprocess Attributes Using Genome‐Scale Models of CHO MetabolismShangzhong Li, Anne Richelle, Nathan E. Lewis · 2019 · Wiley eBooks
- In Vitro Ovarian Model SystemsPj Devine, S. K. Petrillo, Rita G Cortvrindt, L. Rasmussen, Emma Paunil, Zelieann Rivera Craig · 2016 · Elsevier eBooks
- Geração de novas variantes de fator IX recombinante humano com aumento da sua atividade biológicaAline de Sousa Bomfim · 2018
- Genome-editing techniques to increase the therapeutic efficacy of monoclonal antibodiesVictoria Valerievna Firstova, I. G. Shemyakin, Ivan Alekseevich Dyatlov · 2020 · Annals of the Russian academy of medical sciences
- Glycoengineering of Chinese Hamster Ovary cells for improving biotherapeutics’ efficacies.Cheng‐Yu Chung · 2016
- Genome Editing of EukaryaJonathan Asmund Arnesen, Jakob Blæsbjerg Nielsen, Helene Faustrup Kildegaard, Irina Borodina · 2021 · Metabolic Engineering
- El trabajo de la autonomía de 3 a 6 añosDolors Blanch, Leonor March · 2013 · Aula de infantil
- Monoclonal Antibody Glycoengineering for Biopharmaceutical Quality AssuranceItzcóatl A. Gómez, Ioscani Jiménez del Val · 2021 · Cell engineering
- CRISPR-Cas13d for CHO Cell Engineering and Antibody ProductionMei‐Wei Lin, Chih‐Che Shen, Yen-Ju Lin, Min‐Yuan Chou, Nam Ngoc Pham, Yi‐Hao Chang, Chin-Wei Chang, Jih Ru Hwu, Yu‐Chen Hu · 2022
- Correlating amino acid profiles with improved expression capability of superior CHO based expression platformsVijeta Sharma, Richa Guleria, Krishna Jyoti Mukherjee · 2026 · Indian Journal of Biochemistry and Biophysics
- Fucosylated IgG Contributes to Adipose Tissue Dysfunction During AgingJingyu Wang, Wei Su, Haotian Wang, Licui Liu, Jin‐Long Li, Youxin Wang · 2025 · Engineering
- STABILON enhances recombinant protein expression by mitigating proteolytic degradation in CHO cellsJiaxin Li, Ya-ru Zhao, Qiu-li Sun, Zimeng Han, Yanfang Wang, Xiao han Guo, Tianyun Wang, Tianyun Wang · 2025 · Biochemical Engineering Journal
- Generation of Long-Lived CHO Cells Suitable for Production of Afucosylated Antibodies and Fc-Fusion ProteinsDenis Kolesov, Nadezhda A. Orlova, Ivan Ivanovich Vorobiev · 2025 · Doklady Biological Sciences
- Enhancing TGF-β1 production in CHO cells via MS-275 treatment, Bax/Bak knockout, and PACEsol overexpressionKyungsoo Kim, Kyoung Eun Baek, Gyun Min Lee · 2026 · Biotechnology and Bioprocess Engineering
- The historical evolution of CHO cell line development for biologics production: 60 years and still counting.Juergen Fieder, Stefan Schneider, Simon Fischer · 2026 · Critical Reviews in Biotechnology