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A dual enzyme system composed of a polyester hydrolase and a carboxylesterase enhances the biocatalytic degradation of polyethylene terephthalate films
Abstract
TfCut2 from Thermobifida fusca KW3 and the metagenome‐derived LC‐cutinase are bacterial polyester hydrolases capable of efficiently degrading polyethylene terephthalate (PET) films. Since the enzymatic PET hydrolysis is inhibited by the degradation intermediate mono‐(2‐hydroxyethyl) terephthalate (MHET), a dual enzyme system consisting of a polyester hydrolase and the immobilized carboxylesterase TfCa from Thermobifida fusca KW3 was employed for the hydrolysis of PET films at 60°C. HPLC analysis of the reaction products obtained after 24 h of hydrolysis showed an increased amount of soluble products with a lower proportion of MHET in the presence of the immobilized TfCa. The results indicated a continuous hydrolysis of the inhibitory MHET by the immobilized TfCa and demonstrated its advantage as a second biocatalyst in combination with a polyester hydrolase for an efficient degradation oft PET films. The dual enzyme system with LC‐cutinase produced a 2.4‐fold higher amount of degradation products compared to TfCut2 after a reaction time of 24 h confirming the superior activity of his polyester hydrolase against PET films.
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Cited by
Structural insight into molecular mechanism of poly(ethylene terephthalate) degradation
Joo, S., Cho, I. J., Seo, H., Son, H. F., Sagong, H.-Y., Shin, T. J., Choi, S. Y., Lee, S. Y., & Kim, K.-J. (2018) · Nature Communications · doi:10.1038/s41467-018-02881-1 · Cited by 802
Enzyme discovery and engineering for sustainable plastic recycling
Zhu, B., Wang, D., & Wei, N. (2022) · Trends in Biotechnology · doi:10.1016/j.tibtech.2021.02.008 · Cited by 366
Occurrence, toxicity and remediation of polyethylene terephthalate plastics. A review
Dhaka, V., Singh, S., Anil, A. G., Sunil Kumar Naik, T. S., Garg, S., Samuel, J., Kumar, M., Ramamurthy, P. C., & Singh, J. (2022) · Environmental Chemistry Letters · doi:10.1007/s10311-021-01384-8 · Cited by 331
Mechanism-Based Design of Efficient PET Hydrolases
Wei, R., von Haugwitz, G., Pfaff, L., Mican, J., Badenhorst, C. P. S., Liu, W., Weber, G., Austin, H. P., Bednar, D., Damborsky, J., et al. (2022) · ACS Catalysis · doi:10.1021/acscatal.1c05856 · Cited by 284
Self-citationMicrobial colonization and degradation of marine microplastics in the plastisphere: A review
Zhai, X., Zhang, X.-H., & Yu, M. (2023) · Frontiers in Microbiology · doi:10.3389/fmicb.2023.1127308 · Cited by 281
Metadata
- Authors
- Barth, M., Honak, A., Oeser, T., Wei, R., Belisário‐Ferrari, M. R., Then, J., Schmidt, J., & Zimmermann, W.
- Venue
- Biotechnology Journal
- Publisher
- Wiley
- Published
- 2016-06-21
- Volume
- 11
- Issue
- 8
- Pages
- 1082-1087
- ISSN
- 1860-6768 (ISSN-L)
- 1860-7314
- OpenAlex
- W2400947447
- Cited by
- 225
- References
- 0
- FWCI
- 4.51
- Type
- journal article
- Language
- en
- Open access
- closed
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- Barth, M., Honak, A., Oeser, T., Wei, R., Belisário‐Ferrari, M. R., Then, J., Schmidt, J., & Zimmermann, W. (2016). A dual enzyme system composed of a polyester hydrolase and a carboxylesterase enhances the biocatalytic degradation of polyethylene terephthalate films. Biotechnology Journal. https://doi.org/10.1002/biot.201600008
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