Identification of a metabolic network structure representative of arthrospira (spirulina) platensis metabolism
Abstract
A comprehensive network structure for the autotrophic growth of Arthrospira platensis is proposed. The metabolic network was built up with 121 reactions and 134 metabolites including biomass synthesis, production of a growth-associated exopolysaccharide, and energy aspects. The model supports the existence of a metabolic shunt of PEP to pyruvate through PEP carboxylase, NAD(+)-dependent malate dehydrogenase and malic enzyme to convert NADH,H(+) into NADPH,H(+). A limit in Arthrospira growth metabolism due to NADH,H(+) balancing is evidenced, explaining why the maximal light-dependent mass yield of the growth-associated exopolysaccharide was 0.51 kg EPS kg(-1) biomass, consistent with experimental results.
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The exopolysaccharide mass fraction predicted by the LTIP approach agrees with experimental observations and metabolic analysis of Limnospira indica PCC8005 growth under different incident light fluxes.
“The mass fraction of exopolysaccharide that is predicted using the LTIP approach (Figure 5b) is consistent with experimental observations and metabolic analysis of Limnospira indica PCC 8005 growth for different light incident fluxes conditions (Cogne et al. 2003 a ).”
From Limnospira indica PCC8005 growth in photobioreactor: model and simulation of the ISS and ground experiments (2020) · cites this paper as Cogne et al. 2003 a
Oxygen production measured from gas pressure was shown to be consistent with the stoichiometry of growth.
“Cogne et al (2003 c ) have shown that the oxygen production measured by this way is consistent with the stoichiometry of the growth.”
From Limnospira indica PCC8005 growth in photobioreactor: model and simulation of the ISS and ground experiments (2020) · cites this paper as Cogne et al (2003 c )
This confirms the predictability of the model, which was already applied for various ground photobioreactors.
“This confirms the predictability of the model, which was already applied for various ground photobioreactors (Cogne et al., 2003 b , Cornet et al., 2009, Ifrim et al., 2014, Dauchet et al., 2016).”
From Limnospira indica PCC8005 growth in photobioreactor: model and simulation of the ISS and ground experiments (2020) · cites this paper as Cogne et al., 2003 b
Cites
PHOTOTROPHIC PROKARYOTES: THE CYANOBACTERIA
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“All the enzymes that mediate the reactions shown are synthesized under both light and dark growth conditions (Stanier and Cohen-Bazire, 1977).”
BackgroundSpirulina Platensis Arthrospira
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Self-citationNew applications of synthetic biology tools for cyanobacterial metabolic engineering
Santos-Merino, M., Singh, A. K., & Ducat, D. C. (2019) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2019.00033 · Cited by 222 · see citing works
A review on photobioreactor design and modelling for microalgae production
Legrand, J., Artu, A., & Pruvost, J. (2021) · Reaction Chemistry & Engineering · doi:10.1039/d0re00450b · Cited by 125 · see citing works
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- Authors
- Cogne, G., Gros, J. ‐., & Dussap, C. ‐.
- Affiliations
- Université Blaise Pascal (FR)
- Corresponding author
- Cogne, Guillaume
- Venue
- Biotechnology and Bioengineering
- Publisher
- Wiley
- Published
- 2003-10-07
- Volume
- 84
- Issue
- 6
- Pages
- 667-676
- ISSN
- 0006-3592 (ISSN-L)
- 1097-0290
- OpenAlex
- W2020105605
- Cited by
- 71
- References
- 3
- Citations by year
- 2026: 2 · 2025: 1 · 2024: 4 · 2023: 1 · 2022: 5 · 2021: 9 · 2020: 3 · 2019: 3 · 2018: 4 · 2017: 4 · 2016: 4 · 2015: 4 · 2014: 2 · 2013: 2 · 2012: 5
- FWCI
- 0.23
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biotechnology and Bioengineering (publishedVersion)
- PubMed (publishedVersion)
- HAL (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gene Regulatory Network Analysis
- Keywords
- Spirulina platensis, Spirulina, metabolic network model, autotrophic growth, exopolysaccharide production, phosphoenolpyruvate carboxylase, malic enzyme, malate dehydrogenase, biomass synthesis
- MeSH terms
- Cyanobacteria, Cell Division, Computer Simulation, Energy Metabolism, Models, Biological, Multienzyme Complexes, Polysaccharides, Signal Transduction, Photobiology
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