Construction of a Genome-Scale Metabolic Model of Arthrospira platensis NIES-39 and Metabolic Design for Cyanobacterial Bioproduction
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Arthrospira (Spirulina) platensis is a promising feedstock and host strain for bioproduction because of its high accumulation of glycogen and superior characteristics for industrial production. Metabolic simulation using a genome-scale metabolic model and flux balance analysis is a powerful method that can be used to design metabolic engineering strategies for the improvement of target molecule production. In this study, we constructed a genome-scale metabolic model of A. platensis NIES-39 including 746 metabolic reactions and 673 metabolites, and developed novel strategies to improve the production of valuable metabolites, such as glycogen and ethanol. The simulation results obtained using the metabolic model showed high consistency with experimental results for growth rates under several trophic conditions and growth capabilities on various organic substrates. The metabolic model was further applied to design a metabolic network to improve the autotrophic production of glycogen and ethanol. Decreased flux of reactions related to the TCA cycle and phosphoenolpyruvate reaction were found to improve glycogen production. Furthermore, in silico knockout simulation indicated that deletion of genes related to the respiratory chain, such as NAD(P)H dehydrogenase and cytochrome-c oxidase, could enhance ethanol production by using ammonium as a nitrogen source.
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Yoshikawa et al. observed a positive correlation of glycogen content with increasing light intensity, reaching 42% DW at 250 μmol photons m−2 s−1.
“They observed a positive correlation of the glycogen content with the increasing light intensity, reaching 42% DW for 250 μmol photons m 2 s -1 .”
From Arthrospira platensis as a Feasible Feedstock for Bioethanol Production (2021) · cites this paper as [59]
Addition of NaCl in the culture medium could affect the transcriptional regulation of the metabolism of different cyanobacteria via cAMP as a signaling molecule.
“The addition of NaCl in the culture medium could also affect the transcriptional regulation of the metabolism of different cyanobacteria via cAMP as a signaling molecule [59]”
From Arthrospira platensis as a Feasible Feedstock for Bioethanol Production (2021) · cites this paper as [59]
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- Authors
- Yoshikawa, K., Aikawa, S., Kojima, Y., Toya, Y., Furusawa, C., Kondo, A., & Shimizu, H.
- Affiliations
- Japan Science and Technology Agency (JP)
- The University of Osaka (JP)
- Corresponding author
- Shimizu, Hiroshi
- ORCID
- Venue
- PLOS ONE
- Publisher
- Public Library of Science (PLoS)
- Published
- 2015-12-07
- Volume
- 10
- Issue
- 12
- Pages
- e0144430
- ISSN
- 1932-6203 (ISSN-L)
- PMC
- PMC4671677
- OpenAlex
- W2265324020
- Cited by
- 39
- References
- 49
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 10 · 2023: 2 · 2022: 5 · 2021: 4 · 2020: 3 · 2019: 4 · 2018: 3 · 2017: 4 · 2016: 1
- FWCI
- 1.16
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- PLoS ONE (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- RePEc: Research Papers in Economics (submittedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Figshare (submittedVersion, cc-by)
- Funders
- Japan Society for the Promotion of Science
- Japan Science and Technology Agency
- Core Research for Evolutional Science and Technology
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gene Regulatory Network Analysis
- Keywords
- Spirulina platensis, genome-scale metabolic models, glycogen synthesis, ethanol production, metabolic engineering, TCA cycle, autotrophic growth, ammonium nitrogen source, NAD(P)H dehydrogenase, cytochrome c oxidase, flux balance analysis
- MeSH terms
- Ethanol, Biochemical Phenomena, Citric Acid Cycle, Electron Transport Complex IV, Glycogen, Models, Biological, Phosphoenolpyruvate, Genome, Bacterial, Bioreactors, FMN Reductase, Spirulina, Metabolic Networks and Pathways, Gene Knockout Techniques, Metabolic Engineering, Ammonium Compounds
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
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