The DOI matches its Crossref record; 8 of the 8 references it cites were found in a registry or an index.
Engineering Chinese hamster ovary cells to maximize effector function of produced antibodies using FUT8 siRNA
Abstract
We explored the possibility of converting established antibody‐producing cells to cells producing high antibody‐dependent cellular cytotoxicity (ADCC) antibodies. The conversion was made by constitutive expression of small interfering RNA (siRNA) against α1,6 fucosyltransferase (FUT8). We found two effective siRNAs, which reduce FUT8 mRNA expression to 20% when introduced into Chinese hamster ovary (CHO)/DG44 cells. Selection for Lens culinaris agglutinin (LCA)‐resistant clones after introduction of the FUT8 siRNA expression plasmids yields clones producing highly defucosylated (≈60%) antibody with over 100‐fold higher ADCC compared to antibody produced by the parental cells (≈10% defucosylated). Moreover, the selected clones remain stable, producing defucosylated antibody even in serum‐free fed‐batch culture. Our results demonstrate that constitutive FUT8 siRNA expression can control the oligosaccharide structure of recombinant antibody produced by CHO cells to yield antibodies with dramatically enhanced ADCC. © 2004 Wiley Periodicals, Inc.
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Cites
Identification of Novel Genes Coding for Small Expressed RNAs
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Inhibitory Fc receptors modulate in vivo cytoxicity against tumor targets
Clynes, R. A., Towers, T. L., Presta, L. G., & Ravetch, J. V. (2000) · Nature Medicine · doi:10.1038/74704 · Cited by 2,768 · cited 2×
“Since antibody effector function strictly depends on the Fc region carbohydrate structure (Clynes et al., 2000; Lewis et al., 1993), we apFigure 4.”
BackgroundExpression profiling reveals off-target gene regulation by RNAi
Jackson, A. L., Bartz, S. R., Schelter, J., Kobayashi, S. V., Burchard, J., Mao, M., Li, B., Cavet, G., & Linsley, P. S. (2003) · Nature Biotechnology · doi:10.1038/nbt831 · Cited by 2,370
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Shinkawa, T., Nakamura, K., Yamane, N., Shoji-Hosaka, E., Kanda, Y., Sakurada, M., Uchida, K., Anazawa, H., Satoh, M., Yamasaki, M., et al. (2003) · Journal of Biological Chemistry · doi:10.1074/jbc.m210665200 · Cited by 1,413
Self-citationUmaña, P., Jean–Mairet, J., Moudry, R., Amstutz, H., & Bailey, J. E. (1999) · Nature Biotechnology · doi:10.1038/6179 · Cited by 764
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The biological activity of human monoclonal IgG anti-D is reduced by β-galactosidase treatment
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Li, F., Vijayasankaran, N., Shen, A. (., Kiss, R., & Amanullah, A. (2010) · mAbs · doi:10.4161/mabs.2.5.12720 · Cited by 739
Liu, L. (2015) · Journal of Pharmaceutical Sciences · doi:10.1002/jps.24444 · Cited by 428
Control of Recombinant Monoclonal Antibody Effector Functions by Fc N‐Glycan Remodeling in Vitro
Hodoniczky, J., Zheng, Y. Z., & James, D. C. (2005) · Biotechnology Progress · doi:10.1021/bp050228w · Cited by 393
Kanda, Y., Yamada, T., Mori, K., Okazaki, A., Inoue, M., Kitajima-Miyama, K., Kuni-Kamochi, R., Nakano, R., Yano, K., Kakita, S., et al. (2007) · Glycobiology · doi:10.1093/glycob/cwl057 · Cited by 372
Self-citationPereira, N. A., Chan, K. F., Lin, P. C., & Song, Z. (2018) · mAbs · doi:10.1080/19420862.2018.1466767 · Cited by 334
Metadata
- Authors
- Mori, K., Kuni‐Kamochi, R., Yamane‐Ohnuki, N., Wakitani, M., Yamano, K., Imai, H., Kanda, Y., Niwa, R., Iida, S., Uchida, K., Shitara, K., & Satoh, M.
- Venue
- Biotechnology and Bioengineering
- Publisher
- Wiley
- Published
- 2004-10-28
- Volume
- 88
- Issue
- 7
- Pages
- 901-908
- ISSN
- 0006-3592 (ISSN-L)
- 1097-0290
- OpenAlex
- W2129658913
- Cited by
- 174
- References
- 8
- FWCI
- 3.39
- Type
- journal article
- Language
- en
- Open access
- closed
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- Mori, K., Kuni‐Kamochi, R., Yamane‐Ohnuki, N., Wakitani, M., Yamano, K., Imai, H., Kanda, Y., Niwa, R., Iida, S., Uchida, K., Shitara, K., & Satoh, M. (2004). Engineering Chinese hamster ovary cells to maximize effector function of produced antibodies using FUT8 siRNA. Biotechnology and Bioengineering. https://doi.org/10.1002/bit.20326
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