Identification of Novel Genes Coding for Small Expressed RNAs
Open accessAbstract
In Caenorhabditis elegans, lin-4 and let-7 encode 22- and 21-nucleotide (nt) RNAs, respectively, which function as key regulators of developmental timing. Because the appearance of these short RNAs is regulated during development, they are also referred to as small temporal RNAs (stRNAs). We show that many 21- and 22-nt expressed RNAs, termed microRNAs, exist in invertebrates and vertebrates and that some of these novel RNAs, similar to let-7 stRNA, are highly conserved. This suggests that sequence-specific, posttranscriptional regulatory mechanisms mediated by small RNAs are more general than previously appreciated.
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The panel of organs tested did not include all the organs and tissues that miRNAs were cloned from, and thus some organ-specific or organ-enriched miRNAs may be expressed in additional organs or tissues.
“The panel of organs that we tested in our study did not include all the organs and tissues that miRNAs were cloned from [1-3,9,10], and thus some organ-specific or organ-enriched miRNAs may be expressed in additional organs or tissues.”
This study confirms the expression of previously cloned miRNAs.
“This study confirms the expression of previously cloned miRNAs [1-3,9,10]”
miR-9 is also down-regulated during development of the fly, the organism from which it was initially cloned.
“Interestingly, miR-9 is also down-regulated during development of the fly, the organism from which it was initially cloned (Lagos-Quintana et al. 2001).”
From A microRNA array reveals extensive regulation of microRNAs during brain development (2003) · cites this paper as Lagos-Quintana et al. 2001
Some miRNAs are organized in the genome as clusters sometimes separated by intervals as short as a few nucleotides.
“Some miRNAs are organized in the genome as clusters sometimes separated by intervals as short as a few nucleotides (Lagos-Quintana et al. 2001;Lau et al. 2001).”
From A microRNA array reveals extensive regulation of microRNAs during brain development (2003) · cites this paper as Lagos-Quintana et al. 2001
An 87-bp eutherian-specific CNE corresponds to hsa-mir-28, which was previously detected by northern blot in human and mouse but not in non-mammalian species.
“An example is an 87-bp eutherian-specific CNE corresponding to hsa-mir-28; it has previously been detected by northern blot analysis in human and mouse, but not in any non-mammalian species 79 .”
From Genome of the marsupial Monodelphis domestica reveals innovation in non-coding sequences (2007) · cites this paper as [79]
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References 28 · Cited by 4,956
- Authors
- Lagos-Quintana, M., Rauhut, R., Lendeckel, W., & Tuschl, T.
- Affiliations
- Department of Cellular Biochemistry, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, D-37077 Göttingen, Germany.
- Max Planck Institute for Biophysical Chemistry (DE)
- Corresponding author
- Tuschl, Thomas
- ORCID
- Tuschl, Thomas
- Venue
- Science
- Published
- 2001-10-26
- Volume
- 294
- Issue
- 5543
- Pages
- 853-858
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2151922790
- Cited by
- 4,956
- References
- 28
- Citations by year
- 2026: 37 · 2025: 94 · 2024: 127 · 2023: 132 · 2022: 144 · 2021: 199 · 2020: 201 · 2019: 203 · 2018: 217 · 2017: 255 · 2016: 220 · 2015: 287 · 2014: 313 · 2013: 323 · 2012: 301
- FWCI
- 41.92
- Type
- journal article
- Language
- en
- Open access
- green
- hdl.handle.net
- Licence
- other-oa
- Locations
- Science (publishedVersion)
- PubMed (publishedVersion)
- Max Planck Institute for Plasma Physics (submittedVersion)
- MPG.PuRe (Max Planck Society) (submittedVersion, other-oa)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › MicroRNA in disease regulation
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
- Keywords
- microRNA, Caenorhabditis elegans, lin-4, let-7 microRNA, developmental timing, post-transcriptional regulation
- MeSH terms
- Animals, Cell Line, Cloning, Molecular, Drosophila melanogaster, Gene Expression Regulation, Multigene Family, HeLa Cells, Humans, Nucleic Acid Conformation, Organ Specificity, RNA, RNA Precursors, Blotting, Northern, Conserved Sequence, Gene Expression Regulation, Developmental, Evolution, Molecular, Computational Biology, Base Pairing, Gene Expression Profiling, RNA, Untranslated
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