Analysis and comparison of oxygen consumption of HepG2 cells in a monolayer and three-dimensional high density cell culture by use of a matrigrid®
Abstract
By the use of a MatriGrid® we have established a three-dimensional high density cell culture. The MatriGrid® is a culture medium permeable, polymeric scaffold with 187 microcavities. In these cavities (300 μm diameter and 207 μm deep) the cells can growth three-dimensionally. For these experiments we measured the oxygen consumption of HepG2 cell cultures in order to optimize cultivation conditions. We measured and compared the oxygen consumption, growth rate and vitality under three different cultivation conditions: monolayer, three-dimensional static and three-dimensional actively perfused. The results show that the cells in a three-dimensional cell culture consume less oxygen as in a monolayer cell culture and that the actively perfused three-dimensional cell culture in the MatriGrid® has a similar growth rate and vitality as the monolayer culture.
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The oxygen consumption of HepG2/C3A cells can be described with Michaelis-Menten kinetics.
“The oxygen consumption of HepG2/C3A cells can be described with the Michaelis-Menten kinetics [41,42]:”
From A liver-on-a-chip platform with bioprinted hepatic spheroids (2016) · cites this paper as [41]
Hep G2 cells' oxygen consumption rate per tissue volume, 4 × 10-17 mol cell-1 s-1 at an estimated compact cell density of 1.1 × 109 cm-3 yielding 4 × 10-8 mol cm-3 s-1, is a good approximation of human liver tissue.
“Hep G2 cells were used in this part of the study, since their oxygen consumption rate per tissue volume, 4 × 10 -17 mol cell -1 s -1 [29] at an estimated compact cell density of 1.1 × 10 9 cm -3 (cell volume of 850 μm 3 [30] ) yielding 4 × 10 -8 mol cm -3 s -1 , is a good approximation of human liver tissue.”
From 3D microperfusion of mesoscale human microphysiological liver models improves functionality and recapitulates hepatic zonation (2023) · cites this paper as [29]
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References 22 · Cited by 25
- Authors
- Weise, F., Fernekorn, U., Hampl, J., Klett, M., & Schober, A.
- Affiliations
- Technische Universität Ilmenau (DE)
- Corresponding author
- Weise, Frank
- Venue
- Biotechnology and Bioengineering
- Publisher
- Wiley
- Published
- 2013-04-22
- Volume
- 110
- Issue
- 9
- Pages
- 2504-2512
- ISSN
- 0006-3592 (ISSN-L)
- 1097-0290
- OpenAlex
- W1676406229
- Cited by
- 25
- References
- 22
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 1 · 2023: 2 · 2022: 2 · 2021: 1 · 2020: 4 · 2019: 2 · 2018: 1 · 2017: 1 · 2016: 3 · 2015: 4 · 2014: 1 · 2013: 1
- FWCI
- 1.65
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biotechnology and Bioengineering (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Physical Sciences › Engineering › Biomedical Engineering › Fluid Dynamics and Mixing
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Keywords
- oxygen consumption, HepG2 cells, 3D cell culture, high cell density culture, monolayer culture, cell growth rate, cell viability
- MeSH terms
- Culture Media, Equipment Design, Humans, Models, Biological, Oxygen, Oxygen Consumption, Cell Culture Techniques, Bioreactors, Hep G2 Cells
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