A novel serum microRNA signature to screen esophageal squamous cell carcinoma
Open accessAbstract
Circulating microRNAs (miRNAs) have been used as promising diagnostic biomarkers for esophageal squamous cell carcinoma (ESCC). We performed miRNA expression profiling using quantitative reverse transcription polymerase chain reaction (qRT‐PCR) based Exiqon panels from three ESCC pools and one normal control (NC) pool samples. Using qRT‐PCR, identified serum miRNAs were further confirmed in training (32 ESCC vs. 32 NCs) and testing stages (108 ESCC vs. 96 NCs). Consequently, five serum miRNAs (miR‐20b‐5p, miR‐28‐3p, miR‐192‐5p, miR‐223‐3p, and miR‐296‐5p) were significantly overexpressed in ESCC compared with NCs. The diagnostic value of the 5‐miRNA signature was validated by an external cohort (60 ESCC vs. 60 NCs). The areas under the receiver operating characteristic curve (ROC) of the 5‐miRNA signature were 0.753, 0.763, and 0.966 for the training, testing, and the external validation stages, respectively. The expression levels of the miRNAs were also determined in tissues, arterial serum, and exosomes. MiR‐20b‐5p, miR‐28‐3p, and miR‐192‐5p were significantly upregulated in ESCC tissues, while miR‐296‐5p was overexpressed in ESCC serum exosomes. In conclusion, we identified a 5‐miRNA signature in serum for the detection of ESCC.
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microRNA's presence in exosomes has been reported in different cells and cancer types.
“microRNA's presence in exosomes have been already reported in different cells and cancer types (44–50).”
From Potential Therapeutic Effects of Exosomes Packed With a miR-21-Sponge Construct in a Rat Model of Glioblastoma (2019) · cites this paper as [46]
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Tumor-Suppressive miR-192-5p Has Prognostic Value in Pancreatic Ductal Adenocarcinoma
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“A previous study has found that five serum miRNAs show elevated expressions in esophageal squamous cell carcinoma (ESCC), which may also be employed as diagnostic markers for ESCC.(34) Taken together, these studies reveal the oncogenic features of miR-20b-5p.”
BackgroundInfluentialIdentification of microRNAs as novel biomarkers for esophageal squamous cell carcinoma
Li, C.-Y., Zhang, W., Xiang, J., Wang, X., Li, J., & Wang, J.-L. (2019) · Chinese Medical Journal · doi:10.1097/cm9.0000000000000427 · Cited by 38 · see citing works
The promising role of miR-296 in human cancer
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“For example, the serum exosomes of miR-296-5p were proved to be indicative for ESCC diagnosis and staging, due to its increasing expression in higher-stage cancer cases.(17) These types of serum-based approaches were thought to be more advantageous than the invasive biopsy methods, while the biopsy methods currently s…”
BackgroundInfluentialHigh expression of miR-135b predicts malignant transformation and poor prognosis of gastric cancer
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“miRNA expression alterations between tissue and blood samples have been frequently observed in previous studies.([35-37]) In fact, the expression patterns of circulating miRNAs may greatly differ from their respective parental cells regardless of their origins, and not all circulating miRNAs can be found in the origin…”
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Circulating microRNAs Profile in Patients With Transthyretin Variant Amyloidosis
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“Currently, molecular science technology has been constantly evolving, and microarray and NGS platforms have made it possible to perform comprehensive gene analysis using liquid biopsies [53,59,60,65,66,68,69].”
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miR-28-5p targets MTSS1 to regulate cell proliferation and apoptosis in esophageal cancer
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The importance of hsa-miR-28 in human malignancies
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The Research Advances of Exosomes in Esophageal Cancer
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“Another previous study found that the expres‐ sion levels of miR‐28‐3p were significantly upregulated in esophageal squamous cell carcinoma (ESCC) tissues and it was therefore suggested to be a potential novel serum miRNA marker to screen for ESCC (20).”
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“In one of the studies on miR-28-3p expression, it was found that its levels were increased in both tissue and serum samples in esophageal squamous cell carcinoma [37].”
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“Although the expression of hsa-miR-28-5p is downregulated in B-cell lymphoma, renal cell carcinoma, hepatocellular carcinoma, and colorectal cancer [7, 17–19], the expression level of hsa-miR-28-5p is increased in ovarian, esophageal, and cervical cancer [6, 20, 21].”
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“In addition, the expression level of miR-20b-5p was significantly higher in patients with advanced stage (III and IV) [17].”
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“Furthermore, the chimeric RNA level in saliva exosomes can be used for the first time as a noninvasive biomarker for detecting early and late EC for postoperative monitoring, therapeutic response, and tumor recurrence (41).”
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BackgroundComputational pathology aids derivation of microRNA biomarker signals from Cytosponge samples
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Revisiting Inhibition Effects of miR-28 as a Metastasis Suppressor in Gastrointestinal Cancers
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miRNAs as Epigenetic Cancer Biomarker
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Cancer precision medicine: Focus on gastrointestinal cancer
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- Authors
- Huang, Z., Zhang, L., Zhu, D., Shan, X., Zhou, X., Qi, L., Wu, L., Zhu, J., Cheng, W., Zhang, H., Chen, Y., Zhu, W., Wang, T., & Liu, P.
- Affiliations
- Jiangsu Province Hospital (CN)
- Soochow University (CN)
- Nanjing Jiangning Hospital (CN)
- China Pharmaceutical University (CN)
- Jiangsu Cancer Hospital (CN)
- Department of Gastroenterology The First Affiliated Hospital of Nanjing Medical University 300 Guangzhou Road Nanjing 210029 China
- Corresponding author
- Zhu, Wei
- Wang, Tongshan
- Liu, Ping
- ORCID
- Venue
- Cancer Medicine
- Publisher
- Wiley
- Published
- 2016-12-30
- Volume
- 6
- Issue
- 1
- Pages
- 109-119
- ISSN
- 2045-7634 (ISSN-L)
- PMC
- PMC5269712
- OpenAlex
- W2565838736
- Cited by
- 74
- References
- 38
- Citations by year
- 2025: 1 · 2024: 7 · 2023: 10 · 2022: 10 · 2021: 5 · 2020: 19 · 2019: 7 · 2018: 10 · 2017: 5
- FWCI
- 3.06
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Cancer Medicine (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Funders
- National Natural Science Foundation of China (81672400, 81672788, 81302047, 81370516)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › MicroRNA in disease regulation
- Health Sciences › Medicine › Surgery › Esophageal Cancer Research and Treatment
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
- Keywords
- esophageal squamous cell carcinoma, circulating microRNAs, serum biomarkers, miR-20b-5p, miR-28-3p, miR-192-5p, miR-223-3p, miR-296-5p, exosomes, cancer screening
- MeSH terms
- Esophageal Squamous Cell Carcinoma, Aged, Carcinoma, Squamous Cell, Esophageal Neoplasms, Female, Humans, Male, Middle Aged, Neoplasm Staging, ROC Curve, Biomarkers, Tumor, Up-Regulation, Gene Expression Regulation, Neoplastic, Area Under Curve, Gene Expression Profiling, MicroRNAs, Early Detection of Cancer
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