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Cited by 81RNA and protein synthesis mechanismsFungal and yeast genetics researchMicrotubule and mitosis dynamics

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Yeast polypeptide chain release factors eRF1 and eRF3 are involved in cytoskeleton organization and cell cycle regulation

Valouev, Igor A., Kushnirov, Vitaly V., Ter‐Avanesyan, Michael D.·2002·Cell Motility
DOI 10.1002/cm.10040
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Abstract

Termination of translation in eukaryotes is controlled by two interacting polypeptide chain release factors, eRF1 and eRF3. eRF1 recognizes nonsense codons UAA, UAG, and UGA, while eRF3 stimulates polypeptide release from the ribosome in a GTP- and eRF1-dependent manner. In the yeast Saccharomyces cerevisiae, eRF1 and eRF3 are encoded by the SUP45 and SUP35 genes, respectively. Here we show that in yeast shortage of any one of the release factors was accompanied by a reduction in the levels of the other release factor and resulted in a substantial increase of nonsense codon readthrough. Besides, repression of the genes encoding these factors caused different effects on cell morphology. Repression of the SUP35 gene caused accumulation of cells of increased size with large buds. This was accompanied by the disappearance of actin cytoskeletal structures, impairment of the mitotic spindle structure, and defects in nuclei division and segregation in mitosis. The evolutionary conserved C-terminal domain of eRF3 similar to the elongation factor EF-1alpha was responsible for these effects. Repression of the SUP45 gene caused accumulation of unbudded cells with 2C and higher DNA content, indicating that DNA replication is uncoupled from budding. The data obtained suggest that eRF1 and eRF3 play additional, nontranslational roles in the yeast cell.

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Metadata

Authors
Valouev, I. A., Kushnirov, V. V., & Ter‐Avanesyan, M. D.
Affiliations
Institute of Experimental Cardiology (RU)
Corresponding author
Ter‐Avanesyan, Michael D.
ORCID
Venue
Cell Motility
Publisher
Wiley
Published
2002-06-19
Volume
52
Issue
3
Pages
161-173
ISSN
0886-1544 (ISSN-L)
DOI
10.1002/cm.10040
OpenAlex
W2015153105
Cited by
81
References
0
Citations by year
2026: 1 · 2025: 2 · 2024: 5 · 2023: 1 · 2022: 1 · 2021: 1 · 2020: 3 · 2019: 4 · 2018: 2 · 2017: 2 · 2016: 2 · 2015: 3 · 2014: 5 · 2013: 2 · 2012: 3
FWCI
2.09
Type
journal article
Language
en
Open access
closed
Licence
onlinelibrary.wiley.com/termsAndConditions#vor (vor)
Locations
  • Cell Motility and the Cytoskeleton (publishedVersion)
  • PubMed (publishedVersion)
Funders
Howard Hughes Medical Institute
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
Keywords
eRF1, eRF3, translation termination, cytoskeletal organization, actin cytoskeleton, mitotic spindle, cell cycle regulation, nuclear division, DNA replication, DNA content, cell morphology, Saccharomyces cerevisiae, Sup35, SUP45
MeSH terms
Actins, Cell Cycle, Cytoskeleton, Spindle Apparatus, Peptide Termination Factors, Saccharomyces cerevisiae, Protein Biosynthesis, Codon, Nonsense

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