The DOI matches its Crossref record; 58 of the 59 references it cites were found in a registry or an index.
Gene Expression Analysis of Yeast Strains with a Nonsense Mutation in the eRF3-Coding Gene Highlights Possible Mechanisms of Adaptation
Open accessAbstract
In yeast Saccharomyces cerevisiae, there are two translation termination factors, eRF1 (Sup45) and eRF3 (Sup35), which are essential for viability. Previous studies have revealed that presence of nonsense mutations in these genes leads to amplification of mutant alleles (sup35-n and sup45-n), which appears to be necessary for the viability of such cells. However, the mechanism of this phenomenon remained unclear. In this study, we used RNA-Seq and proteome analysis to reveal the complete set of gene expression changes that occur during cellular adaptation to the introduction of the sup35-218 nonsense allele. Our analysis demonstrated significant changes in the transcription of genes that control the cell cycle: decreases in the expression of genes of the anaphase promoting complex APC/C (APC9, CDC23) and their activator CDC20, and increases in the expression of the transcription factor FKH1, the main cell cycle kinase CDC28, and cyclins that induce DNA biosynthesis. We propose a model according to which yeast adaptation to nonsense mutations in the translation termination factor genes occurs as a result of a delayed cell cycle progression beyond the G2-M stage, which leads to an extension of the S and G2 phases and an increase in the number of copies of the mutant sup35-n allele.
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“Beyond its primary function in translation termination, loss of gSPt1 disrupted key cellular pathways including cell cycle regulation, cytoskeletal organization and mtOR signaling (valouev et al. 2002;chauvin et al. 2007;Maksiutenko et al. 2024).”
From From serendipity to strategy: molecular glue degraders in cancer therapeutics (2025) · cites this paper as Maksiutenko et al. 2024
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1 of the 59 references couldn’t be matched to a known paper.
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- Kaiser, C., Michaelis, S., and Mitchell, A. (1994). Methods in Yeast Genetics: A Cold Spring Harbor Laboratory Course Manual, Cold Spring Harbor Laboratory Press.
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From serendipity to strategy: molecular glue degraders in cancer therapeutics
Darling, S., Bajrami, I., & West, S. C. (2025) · Critical Reviews in Biochemistry and Molecular Biology · doi:10.1080/10409238.2025.2564068 · Cited by 9
“Notably, one of the most affected protein groups were the cDKs which are key regulators of the g2/M cell cycle transition (Maksiutenko et al. 2024). gSPt1 was identified from a large-scale phenotypic screen designed to uncover cRBN-targeting compounds with broad anti-proliferative activity across…”
Identification and Validation of Telomere-Related Gene Signature in Intervertebral Disc Degeneration
Xie, S., Xiao, H., Zhang, F., Lan, Y., & Luo, M. (2024) · Cureus · doi:10.7759/cureus.71735
Metadata
- Authors
- Maksiutenko, E. M., Barbitoff, Y. A., Danilov, L. G., Matveenko, A. G., Zemlyanko, O. M., Efremova, E. P., Moskalenko, S. E., & Zhouravleva, G. A.
- Affiliations
- Russian Academy of Sciences (RU)
- St Petersburg University (RU)
- Corresponding author
- Zhouravleva, Galina A.
- ORCID
- Venue
- International Journal of Molecular Sciences
- Publisher
- MDPI AG
- Published
- 2024-06-07
- Volume
- 25
- Issue
- 12
- Pages
- 6308
- ISSN
- 1422-0067 (ISSN-L)
- PMC
- PMC11203930
- OpenAlex
- W4399434292
- Cited by
- 2
- References
- 59
- Citations by year
- 2025: 1 · 2024: 1
- FWCI
- 0.27
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- International Journal of Molecular Sciences (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Funders
- Russian Science Foundation (23-14-00063)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
- Keywords
- eRF3, SUP45, translation termination factor, cellular adaptation, gene expression changes, cell cycle regulation, anaphase-promoting complex, CDC20, Fkh1, Cdc28, cyclins, DNA synthesis, Saccharomyces cerevisiae
- MeSH terms
- Adaptation, Physiological, Cell Cycle, Peptide Termination Factors, Saccharomyces cerevisiae, Gene Expression Regulation, Fungal, Codon, Nonsense, Saccharomyces cerevisiae Proteins
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