CoChat
Sign Up
Cited by 63Sarcoma Diagnosis and TreatmentCardiac tumors and thrombiSoft tissue tumor case studies

Dedifferentiated liposarcoma and pleomorphic liposarcoma: A comparative study of cytomorphology and MDM2/CDK4 expression on fine‐needle aspiration

Mariño‐Enríquez, Adrián, Hornick, Jason L., Dal Cin, Paola, Cibas, Edmund S., Qian, Xiaohua·2014·Cancer Cytopathology
DOI 10.1002/cncy.21362
Open this paper in CoChat — free account

Abstract

BACKGROUND: Dedifferentiated liposarcoma (DDLPS) and pleomorphic liposarcoma (PLPS) are distinct high-grade liposarcomas. DDLPS is a nonlipogenic sarcoma characterized by amplification of MDM2 and CDK4. PLPS is a high-grade sarcoma containing lipoblasts, characterized by a complex karyotype and a more aggressive clinical course. Rarely, DDLPS shows lipogenic differentiation, mimicking PLPS. The cytomorphologic features of DDLPS and PLPS and the utility of ancillary studies have not been systemically analyzed. METHODS: Cytologic preparations of 25 DDLPS and 13 PLPS, all histologically confirmed, were retrospectively reviewed along with clinical and cytogenetic data. Sample cellularity, vascular architecture, background material, predominant cell morphology, quality of the cytoplasm, and nuclear pleomorphism were compared for both tumor types. Immunohistochemistry for MDM2 and CDK4 was performed on cell blocks and/or core needle biopsies. RESULTS: Fine-needle aspirate smears from both DDLPS and PLPS were variably cellular, composed of cellular clusters and noncohesive cells. Abundant myxoid stroma was present in ∼25% of DDLPS and PLPS cases, whereas branching curvilinear vessels were more common in DDLPS than in PLPS (7 of 25 versus 2 of 13). Tumors were composed of predominantly spindled (18 of 25 DDLPS versus 3 of 13 PLPS) or epithelioid cells (7 of 25 DDLPS versus 6 of 13 PLPS). Pleomorphic cells were predominant in 3 PLPS, and were frequent in both (13 of 25 DDLPS versus 10 of 13 PLPS). The cytoplasm was mostly fibrillary and often vacuolated in both entities. Other features included necrosis, mitoses, and a prominent inflammatory infiltrate. The main cytologic differences were the presence of marked pleomorphism, abundant lipoblasts, and cells with microvacuolated cytoplasm in most PLPS. A total of 24 (96%) and 20 (80%) cases of DDLPS expressed MDM2 and CDK4, respectively, whereas none of the PLPS expressed both markers. Six DDLPS tested showed ring or giant marker chromosomes and/or MDM2 amplification by fluorescence in situ hybridization; 2 PLPS had complex karyotypes. CONCLUSIONS: DDLPS and PLPS exhibit variable and occasionally overlapping cytologic features. The presence of lipoblasts, cells with microvacuolated cytoplasm, and marked pleomorphism are more suggestive of PLPS, but these characteristics can be present in DDLPS. Coexpression of MDM2 and CDK4 distinguishes DDLPS from PLPS.

Integrity signals

Registry

Crossref · 10.1002/cncy.21362

Cites

39 of 39 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Related

Authors
Mariño‐Enríquez, A., Hornick, J. L., Dal Cin, P., Cibas, E. S., & Qian, X.
Affiliations
  • Brigham and Women's Hospital (US)
  • Department of Pathology Brigham and Women's Hospital and Harvard Medical School Boston Massachusetts
Corresponding author
Qian, Xiaohua
ORCID
Venue
Cancer Cytopathology
Publisher
Wiley
Published
2013-11-12
Volume
122
Issue
2
Pages
128-137
ISSN
  • 1934-662X (ISSN-L)
  • 1934-6638
DOI
10.1002/cncy.21362
OpenAlex
W1586740324
Cited by
63
References
39
Citations by year
2026: 2 · 2025: 5 · 2024: 10 · 2023: 6 · 2022: 5 · 2021: 3 · 2020: 4 · 2019: 8 · 2018: 7 · 2016: 3 · 2015: 6 · 2014: 4
FWCI
3.52
Type
journal article
Language
en
Open access
closed
Licence
onlinelibrary.wiley.com/termsAndConditions#vor (vor)
Locations
  • Cancer Cytopathology (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Sarcoma Diagnosis and Treatment
  • Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Cardiac tumors and thrombi
  • Health Sciences › Medicine › Rheumatology › Soft tissue tumor case studies
Keywords
dedifferentiated liposarcoma, pleomorphic liposarcoma, fine needle aspiration cytology, MDM2 amplification, CDK4 amplification, lipoblasts, ring chromosomes, giant chromosomes, myxoid stroma, epithelioid cells, cytomorphologic features, ancillary studies, differential diagnosis, soft tissue sarcoma, fluorescence in situ hybridization, immunohistochemistry, karyology, core needle biopsy
MeSH terms
Adult, Aged, Aged, 80 and over, Chromosome Aberrations, Female, Humans, Immunohistochemistry, Liposarcoma, Male, Middle Aged, Retrospective Studies, In Situ Hybridization, Fluorescence, Biopsy, Fine-Needle, Cyclin-Dependent Kinase 4, Proto-Oncogene Proteins c-mdm2

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review