The dorsal longitudinal flight muscle (DLM) of Drosophila is composed of six muscle fibers (DLM1‐6 from ventral to dorsal) and innervated by five motor neurons (MNs), DLM1‐4 being innervated singly by MN1‐4, and DLM5 and 6 being jointly innervated by MN5. This study identifies and describes the f…