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Performance of Fluorescence Correlation Spectroscopy for Measuring Diffusion and Concentration
Abstract
Fluorescence correlation spectroscopy (FCS) has become an important tool for measuring diffusion, concentration, and molecular interactions of cellular components. The interpretation of FCS data critically depends on the measurement set‐up. Here, we present a rigorous theory of FCS based on exact wave‐optical calculations. Six of the most important optical and photophysical factors that influence FCS are studied: fluorescence anisotropy, cover‐slide thickness, refractive index of the sample, laser‐beam geometry, optical saturation, and pinhole adjustment. Our theoretical framework represents a general attempt to link all relevant parameters of the experimental set‐up with the measured correlation function.
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Citations
Cited by
Fluorescence correlation spectroscopy in living cells
Kim, S. A., Heinze, K. G., & Schwille, P. (2007) · Nature Methods · doi:10.1038/nmeth1104 · Cited by 444
Imaging molecular interactions in living cells by FRET microscopy
Jares-Erijman, E. A., & Jovin, T. M. (2006) · Current Opinion in Chemical Biology · doi:10.1016/j.cbpa.2006.08.021 · Cited by 313
Abyzov, A., Blackledge, M., & Zweckstetter, M. (2022) · Chemical Reviews · doi:10.1021/acs.chemrev.1c00774 · Cited by 215
Metadata
- Authors
- Enderlein, J., Gregor, I., Patra, D., Dertinger, T., & Kaupp, U. B.
- Venue
- ChemPhysChem
- Publisher
- Wiley
- Published
- 2005-11-07
- Volume
- 6
- Issue
- 11
- Pages
- 2324-2336
- ISSN
- 1439-4235 (ISSN-L)
- 1439-7641
- OpenAlex
- W2169305358
- Cited by
- 221
- References
- 0
- FWCI
- 9.20
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: Performance of Fluorescence Correlation Spectroscopy for Measuring Diffusion and Concentration. https://app.cochat.ai/papers/10.1002/cphc.200500414
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- Enderlein, J., Gregor, I., Patra, D., Dertinger, T., & Kaupp, U. B. (2005). Performance of Fluorescence Correlation Spectroscopy for Measuring Diffusion and Concentration. ChemPhysChem. https://doi.org/10.1002/cphc.200500414
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