Viral Vectors for Gene Transfer

Chen, Yong Hong; Keiser, Megan S.; Davidson, Beverly L.

doi:10.1002/cpmo.58

Cited by 146Virus-based gene therapy researchRNA Interference and Gene DeliveryViral Infectious Diseases and Gene Expression in Insects

Viral Vectors for Gene Transfer

Chen, Yong Hong, Keiser, Megan S., Davidson, Beverly L.·2018·Current Protocols in Mouse Biology

DOI 10.1002/cpmo.58

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Abstract

Viral vectors are a promising tool for effective delivery of genetic material into cells. They take advantage of the natural ability of a virus to deliver a genetic payload into cells while being genetically modified such that their ability to replicate is crippled or removed. Here, an updated overview of routinely used viral vectors, including adeno‐associated viruses (AAV), retroviruses/lentiviruses, and adenoviruses (Ads), is provided, as well as perspectives on their advantages and disadvantages in research and gene therapy. © 2018 by John Wiley & Sons, Inc.

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AAV is one of the most common vectors used in gene therapy.
“AAV is one of the most common vectors used in gene therapy.”
From Viral Vector Systems for Gene Therapy: A Comprehensive Literature Review of Progress and Biosafety Challenges (2020) · cites this paper as [19]
AAV vectors have a small packaging capacity that is generally considered to be less than 5 kb.
“Another substantial limitation of AAV vectors is their small packaging capacity that is generally considered to be <5 kb.”
From Viral Vector Systems for Gene Therapy: A Comprehensive Literature Review of Progress and Biosafety Challenges (2020) · cites this paper as [19]
The AV vector now represents 1 of the 3 major viral vector categories in the gene therapy toolbox, along with AAV and lenti/retrovirus vector systems.
“The AV vector now represents 1 of the 3 major viral vector categories in the gene therapy toolbox, along with AAV and lenti/retrovirus vector systems.”
From Viral Vector Systems for Gene Therapy: A Comprehensive Literature Review of Progress and Biosafety Challenges (2020) · cites this paper as [19]
BSL-2 is appropriate for many constructs of HSV.
“BSL-2 is appropriate for many constructs of HSV.”
From Viral Vector Systems for Gene Therapy: A Comprehensive Literature Review of Progress and Biosafety Challenges (2020) · cites this paper as [19]

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Authors: Chen, Y. H., Keiser, M. S., & Davidson, B. L.
Affiliations: Children's Hospital of Philadelphia (US)
Corresponding author: Davidson, Beverly L.
ORCID: Chen, Yong Hong
Keiser, Megan S.
Davidson, Beverly L.
Venue: Current Protocols in Mouse Biology
Publisher: Wiley
Published: 2018-11-28
Volume: 8
Issue: 4
Pages: e58-e58
ISSN: 2161-2617 (ISSN-L)
DOI: 10.1002/cpmo.58
OpenAlex: W2903362002
Cited by: 146
References: 45
Citations by year: 2026: 12 · 2025: 15 · 2024: 26 · 2023: 20 · 2022: 31 · 2021: 23 · 2020: 17 · 2019: 2
FWCI: 1.47
Type: journal article
Language: en
Open access: closed
Licence: onlinelibrary.wiley.com/termsAndConditions#vor (vor)
Locations: Current Protocols in Mouse Biology (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Virus-based gene therapy research
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Viral Infectious Diseases and Gene Expression in Insects
Keywords: viral vectors, gene transfer, gene therapy, adeno-associated virus, retroviruses, lentivirus, adenovirus, viral vector design, gene delivery
MeSH terms: Dependovirus, Animals, Disease Models, Animal, Genetic Vectors, Genetic Therapy, Mice

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Viral Vectors for Gene Transfer

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