The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Cell‐Free Metabolic Engineering: Production of Chemicals by Minimized Reaction Cascades
Abstract
The limited supply of fossil resources demands the development of renewable alternatives to petroleum‐based products. Here, biobased higher alcohols such as isobutanol are versatile platform molecules for the synthesis of chemical commodities and fuels. Currently, their fermentation‐based production is limited by the low tolerance of microbial production systems to the end products and also by the low substrate flux into cell metabolism. We developed an innovative cell‐free approach, utilizing an artificial minimized glycolytic reaction cascade that only requires one single coenzyme. Using this toolbox the cell‐free production of ethanol and isobutanol from glucose was achieved. We also confirmed that these streamlined cascades functioned under conditions at which microbial production would have ceased. Our system can be extended to an array of industrially‐relevant molecules. Application of solvent‐tolerant biocatalysts potentially allows for high product yields, which significantly simplifies downstream product recovery.
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Cites
Non-fermentative pathways for synthesis of branched-chain higher alcohols as biofuels
Atsumi, S., Hanai, T., & Liao, J. C. (2008) · Nature · doi:10.1038/nature06450 · Cited by 1,945
Cited by
Role of Biocatalysis in Sustainable Chemistry
Sheldon, R. A., & Woodley, J. M. (2018) · Chemical Reviews · doi:10.1021/acs.chemrev.7b00203 · Cited by 1,755
Transformation of Biomass into Commodity Chemicals Using Enzymes or Cells
Straathof, A. J. J. (2014) · Chemical Reviews · doi:10.1021/cr400309c · Cited by 459
Synthetic non-oxidative glycolysis enables complete carbon conservation
Bogorad, I. W., Lin, T.-S., & Liao, J. C. (2013) · Nature · doi:10.1038/nature12575 · Cited by 398
Multienzyme Cascade Reactions—Status and Recent Advances
Sperl, J. M., & Sieber, V. (2018) · ACS Catalysis · doi:10.1021/acscatal.7b03440 · Cited by 363
Self-citationThe potential of biofuels from first to fourth generation
Cavelius, P., Engelhart-Straub, S., Mehlmer, N., Lercher, J., Awad, D., & Brück, T. (2023) · PLOS Biology · doi:10.1371/journal.pbio.3002063 · Cited by 224
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Metadata
- Authors
- Guterl, J., Garbe, D., Carsten, J., Steffler, F., Sommer, B., Reiße, S., Philipp, A., Haack, M., Rühmann, B., Koltermann, A., Kettling, U., Brück, T., & Sieber, V.
- Venue
- ChemSusChem
- Publisher
- Wiley
- Published
- 2012-10-19
- Volume
- 5
- Issue
- 11
- Pages
- 2165-2172
- ISSN
- 1864-5631 (ISSN-L)
- 1864-564X
- OpenAlex
- W1974258032
- Cited by
- 251
- References
- 1
- FWCI
- 6.27
- Type
- journal article
- Language
- en
- Open access
- closed
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