A novel Ly6C/Ly6G‐based strategy to analyze the mouse splenic myeloid compartment
Open accessAbstract
Currently, there is no standardized panel for immunophenotyping myeloid cells in mouse spleen using flow cytometry. Markers such as CD11b, CD11c, F4/80, Gr‐1, Ly6C, and Ly6G have long been used to identify various splenic cell myeloid populations. Flow cytometry and fluorescence‐activated cell sorting (FACS) analysis demonstrated that Ly6G/Ly6C markers are superior to Gr‐1 for identifying splenic neutrophils, eosinophils, and subsets of monocytes/macrophages. Moreover, these experiments showed that F4/80 is not required for identifying these myeloid subsets and that many of the commercially available preparations of anti‐F4/80 antibodies stain poorly for this antigen in spleen. Taken together, we have now developed an informative flow cytometry panel that can be combined with other cell markers to further delineate subpopulations of mouse splenic myeloid cells. This panel will be highly useful to investigators in the flow cytometry field, as there is a critical need to standardize the analysis of myeloid cell subsets. © 2011 International Society for Advancement of Cytometry
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Newly generated monocytes produced by RSD are less mature and more inflammatory than homeostatic monocytes, expressing high levels of Ly6C and CCR2.
“An interesting characteristic of RSD is that the newly generated monocytes are less mature and more inflammatory than 'homeostatic' monocytes.”
From Repeated Social Defeat, Neuroinflammation, and Behavior: Monocytes Carry the Signal (2017) · cites this paper as Rose et al, 2012
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References 14 · Cited by 347
- Authors
- Rose, S., Misharin, A., & Perlman, H.
- Affiliations
- Northwestern University (US)
- Corresponding author
- Perlman, Harris
- Venue
- Cytometry Part A
- Publisher
- Wiley
- Published
- 2011-12-29
- Volume
- 81A
- Issue
- 4
- Pages
- 343-350
- ISSN
- 1552-4922 (ISSN-L)
- 1552-4930
- PMC
- PMC3987771
- OpenAlex
- W2081374651
- Cited by
- 347
- References
- 14
- Citations by year
- 2026: 12 · 2025: 15 · 2024: 13 · 2023: 23 · 2022: 42 · 2021: 34 · 2020: 21 · 2019: 26 · 2018: 24 · 2017: 30 · 2016: 37 · 2015: 33 · 2014: 17 · 2013: 12 · 2012: 7
- FWCI
- 5.11
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Cytometry Part A (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institutes of Health (1p01hl108795-01, 5t32ar007611-20, NIAID R21, AI092490, AR054796)
- National Heart, Lung, and Blood Institute (P01-HL-108795)
- National Institute of Allergy and Infectious Diseases (R21AI092490)
- National Institute of Arthritis and Musculoskeletal and Skin Diseases (T32 AR07611, AR050250, R01AR050250, T32‐AR‐007611, R01AR054796, AR-054796)
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › Immune cells in cancer
- Life Sciences › Immunology and Microbiology › Immunology › Inflammation biomarkers and pathways
- Life Sciences › Immunology and Microbiology › Immunology › Immunotoxicology and immune responses
- Keywords
- Ly6C, Ly6G, Gr-1, F4/80, neutrophils, eosinophils, monocytes, macrophages, mouse spleen, myeloid cell subsets
- MeSH terms
- Animals, Antigens, Ly, Flow Cytometry, Mice, Inbred C57BL, Spleen, Biomarkers, Immunophenotyping, Myeloid Cells, Mice
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