OMIP‐015: Human regulatory and activated T‐cells without intracellular staining
Open accessAbstract
The present panel was optimized to investigate the frequency and phenotype of regulatory T-cells (T reg ), as well as the activation status of CD4 1 and CD8 1 T-cells in peripheral blood mononuclear cells (PBMC) from healthy individuals, without the use of intracellular staining (i.e., excluding the use of the canonical T reg marker, FoxP3).The panel has been developed using cryopreserved PBMC and we have observed similar results with fresh specimens.Other tissue types have not been tested.
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There is a general consensus that the 236A/E7 and 259D anti-human FOXP3 Ab clones are optimal.
“Several studies have compared different anti-human FOXP3 Ab clones, and although there is some debate, there is a general consensus that the 236A/E7 and 259D clones are optimal [394-398].”
From Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition) (2021) · cites this paper as [397]
The Background says this panel avoids intracellular FoxP3 staining, and the reagent table lists no FOXP3 antibody. Searches for 236A/E7, 259D, and comparisons of FOXP3 antibody clones found no passage assessing either clone or reporting a consensus that they are optimal. (LLM, conf=0.78)
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Self-citationOMIP‐023: 10‐Color, 13 antibody panel for in‐depth phenotyping of human peripheral blood leukocytes
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- Authors
- Mahnke, Y. D., Beddall, M. H., & Roederer, M.
- Affiliations
- National Institutes of Health (US)
- Corresponding author
- Mahnke, Yolanda D.
- Venue
- Cytometry Part A
- Publisher
- Wiley
- Published
- 2012-11-16
- Volume
- 83A
- Issue
- 2
- Pages
- 179-181
- ISSN
- 1552-4922 (ISSN-L)
- 1552-4930
- OpenAlex
- W1998777981
- Cited by
- 34
- References
- 15
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 2 · 2023: 2 · 2022: 1 · 2021: 2 · 2020: 1 · 2019: 6 · 2018: 4 · 2017: 2 · 2016: 4 · 2015: 3 · 2014: 2 · 2013: 2
- FWCI
- 0.98
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- public-domain
- onlinelibrary.wiley.com/termsAndConditions#vor (vor)
- Locations
- Cytometry Part A (publishedVersion, public-domain)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › T-cell and B-cell Immunology
- Life Sciences › Immunology and Microbiology › Immunology › Psoriasis: Treatment and Pathogenesis
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Diabetes and associated disorders
- Keywords
- regulatory T cells, T cell activation, CD4+ T cells, CD8+ T cells, T cell phenotype, peripheral blood mononuclear cells, flow cytometry panel, healthy individuals
- MeSH terms
- Antigens, Differentiation, Flow Cytometry, Fluorescent Antibody Technique, Humans, Phenotype, CD4-Positive T-Lymphocytes, CD8-Positive T-Lymphocytes, T-Lymphocytes, Regulatory, Forkhead Transcription Factors, Interleukin-2 Receptor alpha Subunit, Interleukin-7 Receptor alpha Subunit
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