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Possible alternative strategies to implement basophil activation testing in multicentric studies
Abstract
The Basophil Activation Test (BAT) enables flow cytometry characterization of basophil reactivity against specific allergenic molecules. The focus now revolves around democratizing this tool, but, as blood sample stability could be challenging, after having developed a simplified approach, herein, we aimed to characterize two strategies for implementing BAT in multicentric studies: store and ship blood before or after sample processing. Fresh heparin‐ and EDTA‐anticoagulated whole blood samples followed both BAT workflows: “collect, store, process & analyze” or “collect, process, store & analyze”. Storage temperatures of 18–25 °C or 2–8 °C and preservation times from 0 to 7 days were considered. Interleukin‐3 was also evaluated. With the “collect, store, process & analyze” workflow, heparin‐anticoagulated blood and 18–25 °C storage were better than other conditions. While remaining possible, basophil activation exhibited a possible reactivity decay after 24 h. Under the conditions tested, interleukin‐3 had no role in enhancing basophil reactivity after storage. Conversely, the “collect, process, store & analyze” workflow demonstrated that either heparin‐ or EDTA‐anticoagulated blood can be processed and kept up to 7 days at 18–25 °C or 2–8 °C before being analyzed. Various strategies can be implemented to integrate BAT in multicentric studies. The “collect, store, process & analyze” workflow remains a simplified logistical approach, but depending on time required to ship from the clinical centers to the reference laboratories, it might not be applicable, or should be used with caution. The “collect, process, store & analyze” workflow may constitute a workflow improvement to provide significant flexibility without impact on basophil reactivity.
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“Furthermore, BAT depends on fresh blood samples, as basophils are short-lived, presenting logistical challenges in sample collection and processing [210].”
From Pollen–Food Allergy Syndrome: Allergens, Clinical Insights, Diagnostic and Therapeutic Challenges (2024) · cites this paper as [210]
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Monitoring human basophil activation via CD63 monoclonal antibody 435
Knol, E. F., Mul, F. P. J., Jansen, H., Calafat, J., & Roos, D. (1991) · Journal of Allergy and Clinical Immunology · doi:10.1016/0091-6749(91)90094-5 · Cited by 421 · cited 1×
“The basophil activation test (BAT) has emerged as a valuable test for food allergy (Hemmings et al., 2018; Knol et al., 1991).”
Ocmant, A., Peignois, Y., Mulier, S., Hanssens, L., Michils, A., & Schandené, L. (2007) · Journal of Immunological Methods · doi:10.1016/j.jim.2006.12.002 · Cited by 107 · cited 3×
“Considering that Interleukin-3 was described as able to enhance basophil reactivity (Ebo et al., 2006; Ocmant et al., 2007; Paranjape et al., 2020; Schroeder et al., 2009), its use could help restore basophil reactivity level after storage.”
Pros and Cons of Clinical Basophil Testing (BAT)
Hoffmann, H. J., Knol, E. F., Ferrer, M., Mayorga, L., Sabato, V., Santos, A. F., Eberlein, B., Nopp, A., & MacGlashan, D. (2016) · Current Allergy and Asthma Reports · doi:10.1007/s11882-016-0633-6 · Cited by 50 · cited 1×
“Recommendations indicate that BAT should ideally be performed within 4 h of blood collection, as basophil reactivity decreases considerably over time (Hoffmann et al., 2016; Sturm et al., 2009).”
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Cited by
Sorted by citations
Pollen–Food Allergy Syndrome: Allergens, Clinical Insights, Diagnostic and Therapeutic Challenges
Haidar, L., Bănărescu, C. F., Uța, C., Moldovan, S. I., Zimbru, E.-L., Zimbru, R.-I., Ciurariu, E., Georgescu, M., & Panaitescu, C. (2024) · Applied Sciences · doi:10.3390/app15010066 · Cited by 21
Metadata
- Authors
- Bourgoin, P., Dupont, T., Agabriel, C., Carsin, A., Verles, A., Cabanski, M., Vitaliti, A., & Busnel, J.
- Affiliations
- Global Research Organization Beckman Coulter Life Sciences Marseille France
- Department of Pediatric Emergency Medicine Timone University Hospital, APHM Marseille France
- Department of Pediatric Emergency Medicine Saint Joseph Hospital Marseille France
- BioMarker Development Novartis Institutes for Biomedical Research Basel Switzerland
- ORCID
- Bourgoin, Pénélope
- Venue
- Cytometry Part B: Clinical Cytometry
- Publisher
- Wiley
- Published
- 2024-04-04
- Volume
- 106
- Issue
- 5
- Pages
- 392-404
- ISSN
- 1552-4949
- 1552-4957
- Cited by
- 6
- References
- 3
- Language
- en
- Funders
- Beckman Coulter Foundation
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- Bourgoin, P., Dupont, T., Agabriel, C., Carsin, A., Verles, A., Cabanski, M., Vitaliti, A., & Busnel, J. (2024). Possible alternative strategies to implement basophil activation testing in multicentric studies. Cytometry Part B: Clinical Cytometry. https://doi.org/10.1002/cyto.b.22172
