The DOI matches its Crossref record; 14 of the 14 references it cites were found in a registry or an index.
Identification of the designer steroid Androsta‐3,5‐diene‐7,17‐dione in a dietary supplement
Abstract
A liquid chromatography‐mass spectrometry (LC–MS) screen for known anabolic‐androgenic steroids in a dietary supplement product marketed for “performance enhancement” detected an unknown compound having steroid‐like spectral characteristics. The compound was isolated using high performance liquid chromatography with ultraviolet detection (HPLC–UV) coupled with an analytical scale fraction collector. After the compound was isolated, it was then characterized using gas chromatography with simultaneous Fourier Transform infrared detection and mass spectrometry (GC–FT–IR–MS), liquid chromatography–high resolution accurate mass–mass spectrometry (LC–HRAM–MS) and nuclear magnetic resonance (NMR). The steroid had an accurate mass of m/z 285.1847 (error−0.57 ppm) for the protonated species [M + H]+, corresponding to a molecular formula of C 19 H 24 O 2. Based on the GC–FT–IR–MS data, NMR data, and accurate mass, the compound was identified as androsta‐3,5‐diene‐7,17‐dione. Although this is not the first reported identification of this designer steroid in a dietary supplement, the data provided adds information for identification of this compound not previously reported. This compound was subsequently detected in another dietary supplement product, which contained three additional active ingredients.
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“with one report finding that synthetic oral anabolic-androgenic steroids have been detected in up to 20% of performance-enhancing drugs sold legally over the counter (35,40,41).”
From Non-testosterone management of male hypogonadism: an examination of the existing literature (2020) · cites this paper as [40]
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Cites
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Nutritional supplements: prevalence of use and contamination with doping agents
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Synthetic Androgens as Designer Supplements
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Non-testosterone management of male hypogonadism: an examination of the existing literature
Krzastek, S. C., & Smith, R. P. (2020) · Translational Andrology and Urology · doi:10.21037/tau.2019.11.16 · Cited by 33
“Numerous other compounds with steroid-like structures are not regulated and have been identified in dietary supplements marketed to “enhance performance,” with one report finding that synthetic oral anabolic-androgenic steroids have been detected in up to 20% of performanceenhancing drugs sold legally over the counter…”
BackgroundBrito, D. M., de la Torre, X., Colamonici, C., Curcio, D., & Botrè, F. (2019) · Drug Testing and Analysis · doi:10.1002/dta.2734 · Cited by 11
Designer anabolic steroids: A challenge for toxicologists
Gheddar, L., Ameline, A., Raul, J., & Kintz, P. (2019) · Annales de Toxicologie Analytique · doi:10.1016/j.toxac.2019.07.001 · Cited by 7
Interest of HRMS systems in analytical toxicology: Focus on doping products
Arbouche, N., Bottinelli, C., Gerace, E., & Gheddar, L. (2021) · Annales de Toxicologie Analytique · doi:10.1016/j.toxac.2021.10.001 · Cited by 6
Brito, D. M., de la Torre, X., Parr, M. K., & Botrè, F. (2020) · Rapid Communications in Mass Spectrometry · doi:10.1002/rcm.8834 · Cited by 6
Metadata
- Authors
- Lorenz, L. M., Toomey, V. M., Lanzarotta, A. C., Flurer, R. A., & Falconer, T. M.
- Affiliations
- United States Food and Drug Administration (US)
- Corresponding author
- Lorenz, Lisa M.
- Venue
- Drug Testing and Analysis
- Publisher
- Wiley
- Published
- 2019-05-03
- Volume
- 11
- Issue
- 7
- Pages
- 1109-1115
- ISSN
- 1942-7603 (ISSN-L)
- 1942-7611
- OpenAlex
- W2924990831
- Cited by
- 9
- References
- 14
- Citations by year
- 2025: 1 · 2024: 1 · 2023: 1 · 2021: 1 · 2020: 3 · 2019: 2
- FWCI
- 3.02
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Drug Testing and Analysis (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Social Sciences › Sociology and Political Science › Doping in Sports
- Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Toxicology › Forensic Toxicology and Drug Analysis
- Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Pharmacological Effects and Assays
- Keywords
- designer steroids, anabolic steroids, dietary supplements, steroid identification, molecular formula determination, NMR characterization
- MeSH terms
- Androstadienes, Chromatography, High Pressure Liquid, Gas Chromatography-Mass Spectrometry, Magnetic Resonance Spectroscopy, Designer Drugs, Substance Abuse Detection, Spectroscopy, Fourier Transform Infrared, Dietary Supplements
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