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Cited by 287

The DOI matches its Crossref record; 29 of the 29 references it cites were found in a registry or an index.

Properties of four human embryonic stem cell lines maintained in a feeder‐free culture system

Open access
Carpenter, Melissa K., Rosler, Elen S., Fisk, Gregory J., Brandenberger, Ralph, Ares, Ximena, Miura, Takumi, Lucero, Mary, Rao, Mahendra S.·2004·Developmental Dynamics

Abstract

Several laboratories have begun evaluating human ES (hES) cell lines; however, direct comparisons between different hES cell lines have not been performed. We have characterized the properties of four human cell lines maintained in feeder‐free culture conditions. Quantitative assessment of surface markers, microarray analysis of gene expression patterns, expression of SOX‐2, UTF‐1, Rex‐1, OCT3/4, CRIPTO, and telomerase activity demonstrated similar patterns in all hES cell lines examined. Undifferentiated hES cells do not respond to neurotransmitters such as acetylcholine, glutamate, and gamma‐aminobutyric acid. In addition, the undifferentiated hES cells possess gap junctions. Although similarities in marker expression were observed, allotyping showed that all four lines have a distinct HLA profile, predicting differences in transplantation responses. These data provide the first detailed comparison of different hES cell lines and demonstrate remarkable similarities among lines maintained in identical culture conditions. Developmental Dynamics 229:243–258, 2004. © 2003 Wiley‐Liss, Inc.

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Crossref · 10.1002/dvdy.10431

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Metadata

Authors
Carpenter, M. K., Rosler, E. S., Fisk, G. J., Brandenberger, R., Ares, X., Miura, T., Lucero, M., & Rao, M. S.
Venue
Developmental Dynamics
Publisher
Wiley
Published
2003-12-18
Volume
229
Issue
2
Pages
243-258
ISSN
  • 1058-8388 (ISSN-L)
  • 1097-0177
OpenAlex
W2070672203
Cited by
287
References
29
FWCI
10.83
Type
journal article
Language
en
Open access
bronze

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CoChat. (2026). Citation check: Properties of four human embryonic stem cell lines maintained in a feeder‐free culture system. https://app.cochat.ai/papers/10.1002/dvdy.10431
The paper
Carpenter, M. K., Rosler, E. S., Fisk, G. J., Brandenberger, R., Ares, X., Miura, T., Lucero, M., & Rao, M. S. (2004). Properties of four human embryonic stem cell lines maintained in a feeder‐free culture system. Developmental Dynamics. https://doi.org/10.1002/dvdy.10431

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