Tg(Afp‐GFP) expression marks primitive and definitive endoderm lineages during mouse development
Open accessAbstract
Alpha‐fetoprotein (Afp) is the most abundant serum protein in the developing embryo. It is secreted by the visceral endoderm, its derivative yolk sac endoderm, fetal liver hepatocytes, and the developing gut epithelium. The abundance of this protein suggested that Afp gene regulatory elements might serve to effectively drive reporter gene expression in developing endodermal tissues. To this end, we generated transgenic mouse lines Tg(Afp‐GFP) using an Afp promoter/enhancer to drive expression of green fluorescent protein (GFP). Bright GFP fluorescence allowed the visualization, in real time, of visceral endoderm, yolk sac endoderm, fetal liver hepatocytes, and the epithelium of the gut and pancreas. Comparison of the localization of green fluorescence with that of endogenous Afp transcripts and protein indicated that the regulatory elements used to generate these mouse lines directed transgene expression in what appeared to be all Afp‐expressing cells of the embryo, but only in a subset of fetal liver cells. The bright GFP signal permitted flow cytometric analysis of fetal liver hepatocytes. These mice represent a valuable resource for live imaging as well as identification, quantitation, and isolation of cells from the primitive and definitive endoderm lineages of the developing mouse embryo. Developmental Dynamics 235:2549–2558, 2006. © 2006 Wiley‐Liss, Inc.
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13ss (E8.75) is the latest stage at which the visceral endoderm versus definitive endoderm origin of gut tube cells can be unambiguously assigned using Afp-GFP or Ttr-Cre mouse lines.
“13ss (E8.75), approximately corresponding to midgestation, the latest stage analyzed in this study, is the latest stage for unambiguous assignment of visceral endoderm (VE)-vs.-definitive endoderm (DE) origin of gut tube cells using Afp-GFP7,12 or Ttr-Cre7,35 mouse lines.”
From The emergent landscape of the mouse gut endoderm at single-cell resolution (2019) · cites this paper as [12]
The Introduction (page not provided) identifies Afp-GFP mouse lines as tools for tagging and tracking primitive and definitive endodermal cells. It does not establish that 13ss (E8.75) is the latest stage for unambiguous assignment of gut-tube cell origin, and the cited passages do not address Ttr-Cre. (LLM, conf=0.86)
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References 43 · Cited by 80
Research works printed but not linked: 6
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- Authors
- Kwon, G. S., Fraser, S. T., Eakin, G. S., Mangano, M., Isern, J., Sahr, K. E., Hadjantonakis, A., & Baron, M. H.
- Affiliations
- Cornell University (US)
- Icahn School of Medicine at Mount Sinai (US)
- Memorial Sloan Kettering Cancer Center (US)
- Corresponding author
- Hadjantonakis, Anna‐Katerina
- Baron, Margaret H.
- Venue
- Developmental Dynamics
- Publisher
- Wiley
- Published
- 2006-05-17
- Volume
- 235
- Issue
- 9
- Pages
- 2549-2558
- ISSN
- 1058-8388 (ISSN-L)
- 1097-0177
- PMC
- PMC1850385
- OpenAlex
- W2016985320
- Cited by
- 80
- References
- 43
- Citations by year
- 2026: 3 · 2025: 2 · 2023: 5 · 2022: 4 · 2021: 2 · 2020: 3 · 2019: 4 · 2018: 5 · 2017: 3 · 2016: 4 · 2015: 5 · 2014: 4 · 2013: 6 · 2012: 8
- FWCI
- 0.68
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Developmental Dynamics (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- Memorial Sloan-Kettering Cancer Center
- National Institutes of Health
- National Heart, Lung, and Blood Institute (R01HL062248, HL-62248)
- National Cancer Institute (R24 CA88302, P30-CA08748-41, P30-CA-08748, R24CA088302, P30CA008748)
- National Institute of Diabetes and Digestive and Kidney Diseases (R01-DK52191, R01DK052191)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Renal and related cancers
- Keywords
- alpha-fetoprotein, GFP reporter expression, visceral endoderm, yolk sac endoderm, intestinal epithelium, pancreas, primitive endoderm, definitive endoderm
- MeSH terms
- alpha-Fetoproteins, Animals, Blastocyst, Endoderm, Female, Mice, Transgenic, Pregnancy, Recombinant Proteins, Yolk Sac, Genes, Reporter, Gene Expression Regulation, Developmental, Hepatocytes, Embryo Culture Techniques, Green Fluorescent Proteins, Mice
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